Pulsed Electric Field-Based Extraction of Total Polyphenols from Sideritis raiseri Using Hydroethanolic Mixtures

Abstract

Polyphenols are an important class of compounds, due to their excellent antioxidant properties. Lately, much effort has been placed into developing new extraction techniques and optimizing them, so that polyphenols can be retrieved more efficiently from the plant materials. One of the most recent advances in extraction techniques is pulsed electric field extraction (PEF). This new technique is environmentally friendly and has the potential to maximize the recovery of compounds from plant tissues. Although the efficiency of PEF depends, among others, on the nature of the solvent used, up to date, there are no reports on the optimization of the PEF extraction of polyphenols, using hydroethanolic solutions of varying content in ethanol. In this study, three hydroethanolic solutions, water, and ethanol were used for the PEF-based extraction of total polyphenols from Sideritis raiseri. Results were conclusive that the 1:1 mixture of ethanol and water can increase by up to 146% the yield of polyphenols in the extract, highlighting the need to study more extensively, in the future, mixtures of solvents and not just plain water.

Unveiling therapeutic efficacy of extract and multi-targeting phytocompounds from Christella dentata (Forssk.) Brownsey & Jermy against multidrug-resistant Pseudomonas aeruginosa

“…46 Ethyl acetate is known for its effectiveness in extracting terpenoids, 47 our research goal extended beyond terpenoids isolation alone. Ethanol, a more polar solvent than ethyl acetate, allows for the extraction of a wider array of bioactive compounds, including polyphenols, 48 flavonoids 49 and other polar constituents in addition to terpenoids which are of significant interest in our investigation. In accordance with our research, bicyclo[4.3.0]nonane, 2,2,6,7-tetramethyl-7-hydroxy have also been identified in the methanol extract of Dicliptera roxburghiana , demonstrating appreciable anticancer characteristics, 50 additionally, this compound has been found in Nicotiana tabacum 51 and in the leaf and stem of Marsilea quadrifolia (L.).…”

Enhancing Antioxidant Properties of Prunus spinosa Fruit Extracts via Extraction Optimization

“…All information regarding the analyses of the extracts for their TPC, total flavonoid content (TFC), total anthocyanins (TA), ascorbic acid content, and their antioxidant activity (assessed using the DPPH free radical scavenging assay and the FRAP assay) and color analysis are given in detail in Supplementary Materials [21][22][23][24].…”

Combination of Pulsed Electric Field and Ultrasound in the Extraction of Polyphenols and Volatile Compounds from Grape Stems

“…The equipment used for the PEF treatment is provided in detail in our previous studies [19,20,23]. In brief, the was comprised of a high voltage power generator (Leybold, LD Didactic GmbH, Huerth, Germany), a digital oscilloscope, a function/arbitrary waveform generator, and a treatment chamber made of two stainless steel plates (10 cm length and 10 cm height) and 1 cm Teflon between them for isolation purposes.…”

“…PEF is an extraction technique, based on electro-permeabilization, aiming at the creation of pores in the cellular membranes, and thus, assist in the extraction of compounds. Owing to its inherent advantages, PEF has been successfully employed for the improved extraction of polyphenols from citrus fruits [21], potato peels [22], Sideritis raiseri [23], Merlot grapes [24], etc. Moreover, it has been shown that the use of PEF in combination with ultrasound can increase the extraction of phenolics from plant by-products [25].…”