Pulse NMR study of the interaction of calcium ion with dipalmitoylphosphatidylcholine lamellae

Shibata, Toshiyuki

doi:10.1016/0009-3084(90)90132-b

Cited by 9 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher order translates in more extended hydrocarbon chains and induces changes in the surface area per molecule [49] as well as in the lipid bilayer thickness. Shibata [18] indicated an increase in the order parameters of the segments both in the polar head group and in the hydrocarbon chain region of DPPC bilayer at CaCl 2 concentrations 1 mM. Huster et al [50] reported an effect of Ca 2+ on the organization of DMPC hydrocarbon chains at c Ca2+ > 15 mM (10 mM NaCl, 10 mM Hepes, pH 7.4, 37 • C), they observed a reduction in the surface area per DMPC molecule (ΔA L = 2.7 Å 2 ) in the liquid-crystalline phase when Ca 2+ ions (15 mM, 37 • C) mediated the binding of anionic polyelectrolyte (sulfate dextrane) to the DMPC bilayers.…”

Section: Resultsmentioning

confidence: 99%

“…The ions bind naturally to negatively charged phospholipids [5,6] but rather weakly to zwitterionic lipids as phosphatidylcholine (PC) and phosphatidylethanolamine. The binding mechanism and the effect of Ca 2+ on PC bilayer has been studied using different physicochemical techniques: diffraction methods [7][8][9][10][11][12], calorimetry [13,14], NMR [15][16][17][18], force measuring method [19], infrared spectroscopy [20], and particle electrophoresis [21,22]. As a result of these studies, it is generally agreed that the preference for Ca 2+ binding weakens with increasing degree of hydrocarbon chain unsaturation and it is also dependent on the phase state of phospholipid (gel > liquid-crystal), with a variety of binding constants ∼1-400 M −1 depending on the lipid and on the experimental method.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca²⁺ ions: A small‐angle neutron scattering study

Uhrı́ková

Teixeira

Lengyel

et al. 2007

Journal of Spectroscopy

View full text Add to dashboard Cite

Dipalmitoylphosphatidylcholine (DPPC) was hydrated in 0.2–60 mM solution of CaCl2in heavy water and thoroughly homogenized by freezing-thawing process. Small-angle neutron scattering (SANS) shows formation of unilamellar vesicles in the range 1–60 mM of CaCl2. From the Kratky–Porod plot ln [I(Q)Q2] vs.Q2of SANS intensityI(Q)in the range of scattering vectorsQcorresponding to the interval 0.001 Å−2≤Q2≤0.006 Å−2, the vesicle bilayer radius of gyrationRgand the bilayer thickness parameterdgwere obtained. The structure of the bilayer displays different behavior for the gel phase and the liquid-crystalline phase: In the gel phase (at 20°C), the values ofdgindicate nonlinear changes in the lipid bilayer thickness, with a maximum at ~5 mM CaCl2. In the liquid-crystalline phase (at 60°C), the parameter of the lipid bilayer thicknessdg=43.2±0.3 Å is constant within the concentration range 1≤cCa≤40 mM. Vesicles prepared at 60 mM CaCl2show within experimental error, the same values ofdgas pure DPPC unilamellar vesicles prepared by extrusion using polycarbonate filter with pores of diameter 500 Å.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca²⁺ ions: A small‐angle neutron scattering study

Uhrı́ková

Teixeira

Lengyel

et al. 2007

Journal of Spectroscopy

View full text Add to dashboard Cite

show abstract

“…Among the wide variety of ions, sodium, calcium, and magnesium appear to be of a particular interest due to their large number of peculiarities. A quantification of their binding to the lipid bilayers of various compositions, places of the ions binding, effects on the membrane structural organization, and processes of the lateral diffusion of molecules, to name but a few, have been studied extensively by the neutron and X-ray scattering and/or diffraction, NMR and infrared spectroscopy, calorimetry, atomic force microscopy, molecular dynamics simulations, and other research methods. − …”

Section: Introductionmentioning

confidence: 99%

Cation–Zwitterionic Lipid Interactions Are Affected by the Lateral Area per Lipid

Kučerka

Ermakova²,

Dushanov

et al. 2020

Langmuir

View full text Add to dashboard Cite

Interactions of the divalent cations Ca2+ and Mg2+ with the zwitterionic lipid bilayers prepared of a fully saturated dipalmitoylphosphatidylcholine (DPPC) or a di-monounsaturated dioleoylphosphatidylcholine (DOPC) were studied by using the neutron scattering methods and molecular dynamics simulations. The effect on the bilayer structural properties confirms the direct interactions in all cases studied. The changes are observed in the bilayer thickness and lateral area. The extent of these structural changes, moreover, suggests various mechanisms of the cation–lipid interactions. First, we have observed a small difference when studying DPPC bilayers in the gel and fluid phases, with somewhat larger effects in the former case. Second, the hydration proved to be a factor in the case of DOPC bilayers, with the larger effects in the case of less hydrated systems. Most importantly, however, there was a qualitative difference between the results of the fully hydrated DOPC bilayers and the others examined. These observations then prompt us to suggest an interaction model that is plausibly governed by the lateral area of lipid, though affected indirectly also by the hydration level. Namely, when the interlipid distance is small enough to allow for the multiple lipid–ion interactions, the lipid–ion–lipid bridges are formed. The bridges impose strong attractions that increase the order of lipid hydrocarbon chains, resulting in the bilayer thickening. In the other case, when the interlipid distance extends beyond a limiting length corresponding to the area per lipid of ∼65 Å2, Mg2+ and Ca2+ continue to interact with the lipid groups by forming the separate ion–lipid pairs. As the interactions proposed affect the lipid membrane structure in the lateral direction, they may prove to play their role in other mechanisms lying within the membrane multicomponent systems and regulating for example the lipid–peptide–ion interactions.

show abstract

“…Saturated-tail membranes such as 1,2-dimyristoyl- sn -glycero-3-phosphocholine (DMPC) and 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) bind Ca 2+ ions more strongly , than do unsaturated-tail membranes such as 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), and gel-phase membranes (frozen acyl chains) bind more divalent cations than do liquid-disordered membranes (melted acyl chains). − ,,,,− The interaction of ions with the membrane surface is limited to the polar headgroup of the phospholipid, , and the binding site of metal cations is limited to the phosphate moiety. − To accommodate cation binding, the headgroups realign ,,− and the resulting lateral pressure compresses the lipid tails , and creates a more ordered tail region. ,,,− Therefore, Ca 2+ binding favors the more ordered state of the membranes, namely, the gel phase, over the liquid-disordered phase and increases the gel-to-liquid phase transition temperature, T m . ,,,,,− Other structural effects of Ca 2+ binding include a decrease in the area per lipid headgroup ,,, and a slight increase in membrane thickness. , …”

Section: Introductionmentioning

confidence: 99%

The Structure of Ions and Zwitterionic Lipids Regulates the Charge of Dipolar Membranes

et al. 2011

View full text Add to dashboard Cite

In pure water, zwitterionic lipids form lamellar phases with an equilibrium water gap on the order of 2 to 3 nm as a result of the dominating van der Waals attraction between dipolar bilayers. Monovalent ions can swell those neutral lamellae by a small amount. Divalent ions can adsorb onto dipolar membranes and charge them. Using solution X-ray scattering, we studied how the structure of ions and zwitterionic lipids regulates the charge of dipolar membranes. We found that unlike monovalent ions that weakly interact with all of the examined dipolar membranes, divalent and trivalent ions adsorb onto membranes containing lipids with saturated tails, with an association constant on the order of ∼10 M(-1). One double bond in the lipid tail is sufficient to prevent divalent ion adsorption. We suggest that this behavior is due to the relatively loose packing of lipids with unsaturated tails that increases the area per lipid headgroup, enabling their free rotation. Divalent ion adsorption links two lipids and limits their free rotation. The ion-dipole interaction gained by the adsorption of the ions onto unsaturated membranes is insufficient to compensate for the loss of headgroup free-rotational entropy. The ion-dipole interaction is stronger for cations with a higher valence. Nevertheless, polyamines behave as monovalent ions near dipolar interfaces in the sense that they interact weakly with the membrane surface, whereas in the bulk their behavior is similar to that of multivalent cations. Advanced data analysis and comparison with theory provide insight into the structure and interactions between ion-induced regulated charged interfaces. This study models biologically relevant interactions between cell membranes and various ions and the manner in which the lipid structure governs those interactions. The ability to monitor these interactions creates a tool for probing systems that are more complex and forms the basis for controlling the interactions between dipolar membranes and charged proteins or biopolymers for encapsulation and delivery applications.

show abstract

Pulse NMR study of the interaction of calcium ion with dipalmitoylphosphatidylcholine lamellae

Cited by 9 publications

References 46 publications

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca²⁺ ions: A small‐angle neutron scattering study

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca²⁺ ions: A small‐angle neutron scattering study

Cation–Zwitterionic Lipid Interactions Are Affected by the Lateral Area per Lipid

The Structure of Ions and Zwitterionic Lipids Regulates the Charge of Dipolar Membranes

Contact Info

Product

Resources

About

Pulse NMR study of the interaction of calcium ion with dipalmitoylphosphatidylcholine lamellae

Cited by 9 publications

References 46 publications

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca2+ ions: A small‐angle neutron scattering study

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca2+ ions: A small‐angle neutron scattering study

Cation–Zwitterionic Lipid Interactions Are Affected by the Lateral Area per Lipid

The Structure of Ions and Zwitterionic Lipids Regulates the Charge of Dipolar Membranes

Contact Info

Product

Resources

About

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca²⁺ ions: A small‐angle neutron scattering study

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca²⁺ ions: A small‐angle neutron scattering study