Pulse laser-induced generation of cluster codes from metal nanoparticles for           immunoassay applications

Chang, Chia-Yin; Chu, Han‐Wei; Unnikrishnan, Binesh; Peng, Lung-Hsiang; Cang, Jinshun; Hsu, Pang-Hung; Huang, Chih‐Ching

doi:10.1063/1.4976020

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…Our previous study demonstrates that the microporous cellulose membrane provides an excellent environment for homogeneous deposition of nucleic acid-or protein-modified Au NPs. 46,47 not increase significantly after 10 shots per spot, probably because most of the Au NPs in the three-dimensional CAM fibers are fragmented after the 10 shots. Therefore, we chose 10 pulsed laser shots per spot as the optimal laser condition for the following experiments.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

“…Our previous study demonstrates that the microporous cellulose membrane provides an excellent environment for homogeneous deposition of nucleic acid- or protein-modified Au NPs. , Here, the [Au n ] + signals are accumulated from Ab EV71 –Au NPs bound to the highly porous CAM by increasing the number of pulsed laser shots for further amplification of the signal for EV71 detection. [Au 1 ] + signals highly increased with an increase in laser shots per spot from 1 to 10 pulsed laser shots at a power density of 1.83 × 10 4 W cm –2 for the detection of Ab EV71 (0–1.0 × 10 5 PFU mL –1 ) (Figure S8, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

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Nanoparticle-Based LDI-MS Immunoassay for the Multiple Diagnosis of Viral Infections

Chu

Lai

et al. 2019

ACS Sens.

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Many serious public health emergencies around the globe are caused by viral epidemics. Thus, developing a reliable method for viral screening is in high demand. Multiplex assays for simultaneous detection and fast screening of high-risk pathogens are especially needed. This study employs metal nanoparticles to generate specific mass spectral signals for different RNA viruses, which enables simultaneous detection of whole viruses by laser desorption/ionization mass spectrometry (LDI-MS). We developed a nanoparticle-based sandwich immunosorbent assay as a sensing platform for the detection of viruses and viral nonstructural protein by LDI-MS. Cellulose acetate membrane (CAM) serves as the substrate for the fabrication of the sandwich immunosorbent assay with the advantages of clean mass spectra and high enrichment of analytes. Antibody-modified metal nanoparticles (Ab− MNPs; M = Au or Ag) act as metallic biocodes for the LDI-MS detection. The signal amplification readout for the virus is through the pulsed laser-induced formation of metal cluster ions ([M n ] + ; n = 1−3) from the Ab−MNPs which specifically bind on the CAM. Our sensing system is effective for the detection of intact viruses [Enterovirus 71 (EV71) and Japanese encephalitis virus (JEV)], nonstructural protein 1 (NS1) of Zika virus (ZIKV), EV71-spiked human serum samples, and the simultaneous detection of EV71 and ZIKV. Our probe efficiently detects EV71 in real clinical serum samples with >95% agreement with RT-qPCR results. This high-throughput LDI-MS viral detection system is simple, reliable, and high-throughput. We believe this platform has the potential to be employed for the routine screening of patients with viral infections.

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Section: ■ Results and Discussionmentioning

confidence: 94%

“…Our previous study demonstrates that the microporous cellulose membrane provides an excellent environment for homogeneous deposition of nucleic acid- or protein-modified Au NPs. , Here, the [Au n ] + signals are accumulated from Ab EV71 –Au NPs bound to the highly porous CAM by increasing the number of pulsed laser shots for further amplification of the signal for EV71 detection. [Au 1 ] + signals highly increased with an increase in laser shots per spot from 1 to 10 pulsed laser shots at a power density of 1.83 × 10 4 W cm –2 for the detection of Ab EV71 (0–1.0 × 10 5 PFU mL –1 ) (Figure S8, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle-Based LDI-MS Immunoassay for the Multiple Diagnosis of Viral Infections

Chu

Lai

et al. 2019

ACS Sens.

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“…33,34 Modified AuNPs were also used as a probe for the detection of the activity of thrombin. 35 In this case, the intensity of the produced Au cluster ions was correlated to the thrombin concentration. As a result, the probe could specifically detect thrombin with a limit of detection of 2.5 pM in human plasma samples.…”

mentioning

confidence: 84%

“…AuNPs have been proved to be an excellent substrate for the detection of several types of biomolecules, as neutral steroids (testosterone, progesterone and cortisol), carbohydrates (ribose, glucose and maltose), indoleamines (5‐hydroxyindole acetic acid and tryptophan), endogenous (palmitic acid, oleic acid and stearic acid), and exogenous (verampil) chemicals 33,34 . Modified AuNPs were also used as a probe for the detection of the activity of thrombin 35 . In this case, the intensity of the produced Au cluster ions was correlated to the thrombin concentration.…”

Section: Introductionmentioning

confidence: 99%

Untargeted analysis of pure snail slime and snail slime‐induced Au nanoparticles metabolome with MALDI FT‐ICR MS

Onzo

Pascale²,

Acquavia

et al. 2021

J Mass Spectrom

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Chronic wounds result from the failure of the normal wound healing process. Any delay during the tissue repair process could be defined as chronic wound healing, potentially having a highly detrimental impact on human health. To face this problem, in the last years, the use of different technologies alternative to therapeutic agents is gaining more attention. The Helix aspersa snail slime‐based products are increasingly being used for skin injury, thanks to their ability to make tissue repair processes faster. To date, a comprehensive overview of pure snail slime metabolome is not available. Besides, Au nanoparticles (AuNPs) technology is spreading rapidly in the medical environment, and the search for AuNPs “green” synthetic routes that involve natural products as precursor agents is demanded, alongside with a deep comprehension of the kind of species that actively take part in synthesis and product stabilization. The aim of this work is to characterize the metabolic profile of a pure snail slime sample, by an untargeted high‐resolution mass spectrometry‐based analysis. In addition, insights on AuNPs synthesis and stabilization by the main components of pure snail slime used to induce the synthesis were obtained. The untargeted analysis provided a large list of important classes of metabolites, that is, fatty acid derivatives, amino acids and peptides, carbohydrates and polyphenolic compounds that could be appreciated in both samples of slime, with and without AuNPs. Moreover, a direct comparison of the obtained results suggests that mostly nitrogen and sulfur‐bearing metabolites take part in the synthesis and stabilization of AuNPs.

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“…Due to the fact that monometallic nanoparticles can only produce certain cluster ions of certain m/z after fragmentation, like ligand amplification mass tags, metallic barcodes also have the potential for multiplex detection. Our research group demonstrated the use of different metal nanoparticles (Au, Ag and Pt NPs) for the detection of different proteins (thrombin, VEGF, PDGF) [ 48 ]. By using appropriate recognition ligands, multiple metal cluster ion bar codes have huge potential for the analysis of multiple metabolites and drugs simultaneously.…”

Section: Nanomaterials As a Matrix For Saldi-msmentioning

confidence: 99%

Nanoparticle-based laser desorption/ionization mass spectrometric analysis of drugs and metabolites

Chu

Unnikrishnan

Anand

et al. 2018

Journal of Food and Drug Analysis

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Nanoparticle-assisted laser desorption/ionization mass spectrometry (LDI-MS) is a powerful tool for the analysis of a wide range of molecules. Many of the drawbacks in the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) can be avoided with the application of nanomaterials as matrices as well as substrates for the LDI-MS to achieve a low background noise in low m/z region and high reproducibility. Surface-assisted LDI (SALDI)-MS, especially the nanoparticle-based LDI-MS, has emerged as a promising technique for the analysis of trace amounts of substances in various biological samples due to their high surface area for analyte enrichment, efficient desorption/ionization, and homogeneous crystallization of sample. Therefore, it is highly useful in clinical, forensic, medical, food and drug analyses, disease diagnosis, and various other fields. In this review, we briefly discuss the application of various nanomaterials, which include metal-based, carbon-based, silicon-based nanomaterials and nanocomposites, as matrices and substrates for LDI-MS based drug and metabolite analyses and possible detection strategies. Also, we discuss the idea of using "mass tag" for signal amplification for drug and metabolite detection using nanoparticle assisted LDI-MS.

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Pulse laser-induced generation of cluster codes from metal nanoparticles for immunoassay applications

Cited by 5 publications

References 32 publications

Nanoparticle-Based LDI-MS Immunoassay for the Multiple Diagnosis of Viral Infections

Nanoparticle-Based LDI-MS Immunoassay for the Multiple Diagnosis of Viral Infections

Untargeted analysis of pure snail slime and snail slime‐induced Au nanoparticles metabolome with MALDI FT‐ICR MS

Nanoparticle-based laser desorption/ionization mass spectrometric analysis of drugs and metabolites

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