Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin-1 signaling pathway

Liu, Yang; Wu, Hui; Qiu, Weiyu; Guo, Long-Yi; Du, Xin; Yu, Qing-Ying; Gao, Jie; Luo, Songping

doi:10.1016/j.jep.2018.05.018

Cited by 26 publications

(17 citation statements)

References 29 publications

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“…Both Chinese herbs and the gut microbiota had the regulation of innate immunity (Wójcik et al, 2009;Wang et al, 2019). Therefore, Chinese herbs rarely has a direct antibacterial ability, but it can rely on inhibiting bacterial growth proteins to inhibit infection (Si et al, 2016) or enhance the regulatory factors that promote innate immunity to achieve antibacterial effect (Ma et al, 2013(Ma et al, , 2018Si et al, 2016;Wang et al, 2016;Yang et al, 2018;Pascale et al, 2019). Consisting with the long-term research of our labs that the integrity of barrier cells such as epithelial cells and endothelial cells contributed to the bactericidal function of immune cells such as neutrophils (Liu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection

Liu

et al. 2020

Front. Cell. Infect. Microbiol.

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Gut microbiota serves as a critical indicator for gut health during treatment of pathogenic bacterial infection. Both Pulsatilla Decoction (abbreviated to PD, a traditional Chinese medicine compound) and Levofloxacin Hydrochloride (LVX) were known to have therapeutic effects to intestinal infectious disease. However, the changes of gut microbiota after PD or LVX treatment remain unclear. Herein, this work aimed to investigate the changes of intestinal flora after PD or LVX therapy of Escherichia coli infection in rats. Results revealed that PD exhibited a valid therapeutic approach for E. coli infection via the intestinal protection, as well as the inhibited release of IL-8 and ICAM-1. Besides, PD was beneficial to rebuild the gut microbiota via restoring Bacteroidetes spp in the composition of the gut microbiota. Comparatively, LVX treatment promoted the infection and ravaged gut microbiota by significantly decreasing Bacteroidetes and increasing Firmicutes. These findings not only highlight the mechanism of Chinese herbal formula, but extend the application of PD as veterinary medicine, feed additive and pre-mixing agent for improving the production of animal derived foods.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection

Liu

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Degradation of glycerophospholipids by PLA2 can cause AA release (Frisardi et al, 2011). Non-enzymatic and enzymatic oxidation of AA produce several lipid mediators (Yang L. et al, 2018), all of which are closely associated with the glycerophospholipid pathways involved in DHD; this suggests that an interplay among lipids occurs in the colon tissue (Kasuga et al, 2015). The results demonstrated that the level of AA was significantly increased in the model group, whereas a significant decrease in AA was observed when compared to the model group, with restoration to normal level observed in the PD group.…”

Section: Change Of Aa Metabolismmentioning

confidence: 99%

“…Coptis chinensis Franch is a common component of traditional Chinese herbal formulae used to relieve abdominal pain and diarrhea (Tjong et al, 2011); Fraxinus rhynchophylla Hance is commonly used to treat gout, arthritis, diarrhea, and bacillary dysentery in clinics and can remove wetness and heat (Fu et al, 2014); and Huanglian, Huangbai, and Qinpi synergistically enhance the Baitouweng effect of PD. Based on modern pharmacology research, PD can exert anti-fungal effects in vitro (Yang et al, 2017), inhibit inflammatory cytokines (Hu et al, 2009;Yang L. et al, 2018) and prevent damages to pathogenic factors by influencing endothelial glycolysis (Zhang et al, 2014). Based on acute and sub-chronic toxicity, Pulsatilla granules might be considered safe for veterinary use (Jia et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Targeted analysis is mainly used to elucidate specific lipid molecules with great selectivity and specificity achieved in the quantitative analysis (Xu et al, 2018). Therefore, LC-MS is an important tool for the characterization of specific physiological and pathological traits (Giles et al, 2018;Kohno et al, 2018), and lipidomics based on UHPLC-MS has been widely applied to elucidate many biological events (Kohno et al, 2018;Righetti et al, 2018;Xu et al, 2018;Yang L. et al, 2018). As lipid metabolism plays an important role in the process of DHD, some lipid metabolites including LysoPC (P-16:0), LysoPC (P-18:0), LPA (16:0/0:0), LysoPC (22:0) etc.…”

Section: Introductionmentioning

confidence: 99%

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Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Hua

Ma²,

Zhang

et al. 2020

Front. Pharmacol.

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Ethnopharmacological Relevance: Diarrhea is a major medical problem in clinical practice. According to the theory of traditional Chinese medicine (TCM), different types of diarrhea should be treated with different TCM formulations based on the targeted medical condition. Dampness-heat diarrhea (DHD) is a serious diarrheal disease and Pulsatilla decoction (PD), a TCM, has been found effective against DHD. Objective: The aim of this study was to clarify the mechanism of action of PD in DHD using an untargeted lipidomics strategy. Materials and Methods: Wistar rats were randomized to four groups, including the control group, model group, PD groups and self-healing group. The PD groups were given a daily intragastric gavage of PD at doses of 3.76 g/kg. The rat model of DHD established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment, drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of DHD. Then the clinical symptoms and signs, blood routine, serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed to evaluate DHD model and therapeutic effect of PD. Lipid biomarkers of DHD were selected by comparing the control and model groups with the colon lipidomics technology and an ultra-high performance liquid chromatography (UHPLC) coupled with Q Exactive plus mass analyzer. Multivariate statistical analysis and pattern recognition were employed to examine different lipids within the colon of PD-treated rats. Results: The clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of DHD. After treatment with PD, the clinical symptoms and signs of the rats with DHD were improved; the indexes of blood routine and inflammatory cytokines levels tended to be normal. The lipidomics profile of the model group were

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“…Pulsatilla decoction (PD) was first prescribed by ancient Chinese physicians Zhang Zhongjing in his medical book "Shang Han Lun", approximately 1800 years ago. Studies have indicated that PD has multiple therapeutic functions including the anti-C. albicans, anti-diarrhea and anti-inflammatory activity [12][13][14]. However, our previously experiment showed that therapeutic effect of PD on UC was unsatisfactory.…”

Section: Introductionmentioning

confidence: 91%

Modified Pulsatillae decoction inhibits DSS-induced ulcerative colitis in vitro and in vivo via IL-6/STAT3 pathway

Huangfu

Dou

Zhong

et al. 2020

BMC Complement Med Ther

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Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon and rectum, which is positively correlated with the occurrence of IBD-related colorectal cancer (IBD-CRC). Conventional therapies based on drugs such as corticosteroids, mesalamine, and immunosuppression have serious side effects. Pulsatillae decoction (PD) served as a classical prescription for the treatment of colitis in China, has been shown to exert prominent curative effects and good safety. Based on clinical experience and our amelioration, we added an extra herb into this classical prescription, but its therapeutic effect on UC and the underlying mechanism are still unclear. Results: We first found the curative effect of modified PD on dextran sodium sulfate (DSS)-incubated NCM460 cells. Then C57BL/6 mice were administered DSS to induce UC to evaluate the therapeutic of modified PD. The results showed that modified PD alleviated the inflammatory injury, manifested in body weight, colon length, and disease activity index, with histological analysis of colon injury. Transcriptomic sequencing indicated that modified PD treatment downregulated the IL-6/STAT3 signaling pathway, and reduced the levels of p-NF-κB, IL-1β and NLRP3, which were confirmed by western blot. Conclusions: Collectively, our results indict that modified PD could efficiently relieve clinical signs and inflammatory mediators of UC, providing evidence of the anti-colitis effect of modified PD, which might provide novel strategies for therapeutic intervention in UC, which may be applied to the prevention of IBD-CRC.

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Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin-1 signaling pathway

Cited by 26 publications

References 29 publications

Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection

Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Modified Pulsatillae decoction inhibits DSS-induced ulcerative colitis in vitro and in vivo via IL-6/STAT3 pathway

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