Pulsatile to-fro flow induces greater and sustained expression of tissue factor RNA in HUVEC than unidirectional laminar flow

Abe, Ryuzo; Yamashita, Norio; Rochier, Adrienne; Abe, Rei; Nixon, Alexander; Madri, Joseph A.; Sumpio, Bauer E.

doi:10.1152/ajpheart.01197.2010

Cited by 10 publications

(14 citation statements)

References 31 publications

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“…We have previously reported the time course of TF expression of ECs exposed to laminar flow and this started by 2 hr, peaked at 4 hrs and then started to decline by 8 hrs. [7,11,12]. All mechanical stimuli conditions induced higher TF RNA expression compared with static conditions (ANOVA and posthoc analysis of Bonferroni, P<0.05).…”

Section: Discussionmentioning

confidence: 88%

“…HUVECs were exposed to shear stress, utilizing a parallel-plate flow chamber system (Cytodyne, San Diego, CA) as previously described [7,10]. Flow of the perfusion medium was regulated by a computercontrolled syringe pump (PHD 2000 and PHD Ultra Programmable; Harvard Apparatus, Holliston, MA).…”

Section: Mechanical Stress Exposurementioning

confidence: 99%

“…This dose of thrombin results in reproducible stimulation of TF expression [7,[11][12][13][14]. These experiments applying mechanical and chemical stress together were performed simultaneously with the experiments applying only mechanical stress, as paired experiments.…”

Section: Mechanical Stress Exposurementioning

confidence: 99%

“…Our laboratory has investigated the role of mechanical forces on ECs [7][8][9][10], and we have previously reported a significant increase in TF expression of ECs exposed to disturbed flow [7,11,12]. The purpose of this study is to compare the effect of frequency of disturbed and uniform flow on EC expression of TF.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells

Moriguchi¹,

Davis²

2013

J Vasc Med Surg

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We analyzed the effect of change in frequency of uniform or disturbed pulsatile flow on human umbilical vein endothelial cells (HUVECs) in the presence or absence of thrombin (Th). We found that tissue factor (TF) RNA expression in HUVECs exposed to 60 cycles per minute (cpm) of disturbed and uniform flow was significantly higher than that induced by 30 cpm. HUVECs exposed to disturbed flow have significantly higher TF RNA expression than HUVECs exposed to uniform flow at either frequency. We conclude that frequency of pulsatile flow is a critical independent factor in TF RNA expression in HUVECs.

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Section: Discussionmentioning

confidence: 88%

Section: Mechanical Stress Exposurementioning

confidence: 99%

Section: Mechanical Stress Exposurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells

Moriguchi¹,

Davis²

2013

J Vasc Med Surg

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“…11,12 In the present experiment, we exposed HUVECs to laminar pulsatile forward flow (PFF) and continuous forward flow (CFF). To generate CFF (►Fig.…”

Section: Exposure To Flowmentioning

confidence: 99%

Effect of Pulsatile and Continuous Flow on Yes-Associated Protein

et al. 2014

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).Although the risk factors for atherosclerosis are quite variable, localization of atherosclerotic lesions in the arterial tree is surprisingly constant. Atherosclerotic plaques show an increased predilection to areas with complex geometry such as branch points, arches, and bifurcations. Several studies have shown that the pattern of flow in straight portion of the arteries is different from areas of complex geometry. 1,2 While the flow in straight portions of arteries is steady, laminar, and unidirectional, flow in areas of complex geometry is low in magnitude and often changes direction. 3 Although there is evidence that these differences in flow patterns could be responsible for the increased predilection of atherosclerosis to these areas, how these changes are being sensed and transduced to the nucleus is poorly understood. 4Yes-associated protein (YAP) is a recently discovered homolog of Yorkie protein in Drosophila melanogaster which functions as a transcriptional co-activator for transcription enhancer activator domain family member 2 (TEAD II) group of genes that regulate cellular growth. 5 Several studies show that YAP could be involved in the pathogenesis of several diseases including cancers of the breast, pancreas, and liver. 6-8 Some investigators postulate that YAP is a mechanosignaling molecule that transmits mechanical information to the nucleus by detecting changes in actin cytoskeleton induced by changes in shape of the cell. 9 Previous studies have shown that cell shape changes differently with different types of flow. While endothelial cells (ECs) exposed to static conditions were uniformly Keywords ► atherosclerosis ► yes-associated protein ► endothelial cells ► pulsatile flow ► continuous flow ► mechanosignaling AbstractYes-associated protein (YAP) is a mechanosignaling protein that relays mechanical information to the nucleus by changing its level of phosphorylation. We hypothesize that different flow patterns show differential effect on phosphorylated YAP (pYAP) (S127) and total YAP and could be responsible for flow dependent localization of atherosclerosis. Confluent human umbilical vein endothelial cells (HUVECs) seeded on fibronectin-coated glass slides were exposed to continuous forward flow (CFF) and pulsatile forward flow (PFF) using a parallel plate flow chamber system for 30 minutes. Cell lysates were prepared and immunoblotted to detect the levels of phosphorylated YAP and total YAP. HUVECs exposed to both PFF and CFF showed a mild decrease in the levels of both pYAP (S127) and total YAP. While the levels of pYAP (S127) decreased to 87.85 and 85.21% of static control with PFF and CFF, respectively, the levels of total YAP significantly decreased to 91.31 and 92.27% of static control. No significant difference was seen between CFF and PFF on their effect on pYAP (S127), but both conditions resulted in a significant decrease in total YAP at 30 minutes. The results of this experiment show that the possible effect of different types of flow on YAP is not induced before 30 minutes. E...

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Investigating the Effect of Blood Sample Volume in the Chandler Loop Model: Theoretical and Experimental Analysis

Gaamangwe

Peterson

Gorbet

2014

Cardiovasc Eng Tech

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Pulsatile to-fro flow induces greater and sustained expression of tissue factor RNA in HUVEC than unidirectional laminar flow

Cited by 10 publications

References 31 publications

Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells

Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells

Effect of Pulsatile and Continuous Flow on Yes-Associated Protein

Investigating the Effect of Blood Sample Volume in the Chandler Loop Model: Theoretical and Experimental Analysis

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