Pulp tissue reaction to direct pulp capping using two different materials (A histopathological study)

Abstract

Background: Direct pulp capping (DPC) is a mandatory procedure to preserve pulp vitality. Resin modified pulp capping materials represent a category of materials that seal the pulp tissue and maintain its vitality in addition to the resin ability to copolymerize with resin composite restorations. Aim: The aim of this study was to evaluate the pulpal tissue reaction to resin containing calcium silicate base material (TheraCal LC Bisco Inc., Schaumburg, IL, USA) and resin containing light cured calcium hydroxide (Urbical LC Promedica Dental Material GmbH, Germany) when used as direct pulp capping materials. This was achieved through histopathological examination and statistical analysis of the histomorphometric collected data.Materials and methods: 40 (six-week-old male albino rats) were used in this study. All rats undergone direct pulp capping procedure in their maxillary right first molars (20 rats per each capping material). The rats were further divided into two groups according to follow up periods of 7 and 14 days (n=10). For the left maxillary 1 st molars, half of them (n=5) acted as positive control group received pulp exposure procedure that was covered with sterile polytetrafluoroethylene (PTEF;Teflon tape) material. The other half on the left side acted as a negative control. At the end of each experimental period rats were sacrificed. The maxillae were dissected and processed to study the pulpal response to different DPC procedures -microscopically and statistically by the means of inflammatory cells count, calcific barrier area fraction and the mean area fraction of the remaining normal pulp tissue.Results: At 7 days, UrbiCal treated teeth showed active formation of calcific barrier with varying levels of organization in each tooth. The formed barrier is almost resembling a fibrodentin. However, TheraCal treated teeth showed less active formation of calcific barrier. At day 14, Urbical capped pulps showed a more organized and well defined calcific barrier, almost resembling the surrounding normal dentin tissue. TheraCal treated group presented with a well-defined heterogeneous calcific barrier which was closely resemble a fibrodentin. According to our statistical results, the percentage of remaining normal pulp tissue and degree of inflammatory cell infiltration showed superior desirable measurements in Urbical treated groups. Conclusion:Direct pulp capping prolonged the life span of pulpal tissue than if it was left untreated. Urbical pulp capping material provided the exposure site with a more organized calcific barriers, lesser inflammatory reaction with more preserved remaining pulpal tissue than TheraCal did.

Perawatan kaping pulpa indirek disertai restorasi resin komposit pada premolar kedua kanan maksila

“…Beberapa materi kaping pulpa telah diperkenalkan seperti kalsium hidroksida,,MTA, biodentine, calcium silicate cement, light cured calcium silicate based , resin modified glass ionomer. 4,5 TheraCal merupakan bahan berbasis calcium silicate , materi ini memiliki kelebihan setting dengan cepat oleh karena memiliki komponen resin yang dapat dipolimerisasi dengan light cure. 3,6…”