In the late 1800s, cattle began arriving to the Rupununi as an extension of Brazilian ranching activities serving the rubber-boom market of Manaus. The initial impacts of this ranching economy upon the Indigenous peoples of the Rupununi greatly changed their traditional way of life, and in particular affected their access to and control of territory. This was exacerbated in 1920, when the 'cattle trail' was opened to bring beef to the Guyanese market, thereby connecting the region to the country's capital and exposing the Rupununi to coastal peoples, politics, and pressures. Recently, the Brazilian government has been advocating strongly for the development of a continuous surface road linking Manaus through the Rupununi to Georgetown, thereby connecting the Brazilian interior with the Caribbean export economy, with further links to global trade. This road threatens to increase pressures on Indigenous territory within Guyana, resulting in the further annexation of traditional ancestral lands and losses of subsistence and livelihood practices. Based on recent fieldwork, this paper explores the social and environmental impacts of this road upon the peoples of the Rupununi, as well as local responses, and suggests that Brazil may have an increasing presence in the Rupununi, enabled through geopolitical road development.