Pulp ECM-derived macroporous scaffolds for stimulation of dental-pulp regeneration process

Bakhtiar, Hengameh; Pezeshki-Modaress, Mohammad; Kiaipour, Zahra; Shafiee, Mahdieh; Ellini, Mohammad Reza; Mazidi, Amir; Rajabi, Sarah; Benisi, Soheila Zamanlui; Ostad, Seyed Naser; Galler, Kerstin M.; Pakshir, Pardis; Azarpazhooh, Amir; Kishen, Anil

doi:10.1016/j.dental.2019.10.011

Cited by 42 publications

(49 citation statements)

References 23 publications

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“…The findings of this study suggest that DDP seeded with hDPSCs have the potential to provide a promising vascularization promoting strategy for dental pulp regeneration. Although recent studies reported the development of hydrogels consisting of DDP of bovine and human origin (Bakhtiar et al 2020, Li et al 2020, the present study demonstrates the benefit of using DDP in its intact form for dental pulp regeneration via stem cell transplantation where the retained vasculature could facilitate vascularization. The use of stem cell transplantation approach for dental pulp regeneration has shown immense potential for rebuilding the complex histological structure of the native pulp with highly organized pattern (Xuan et al 2018, Sui et al 2019.…”

Section: Discussionmentioning

confidence: 60%

“…Although recent studies reported the development of hydrogels consisting of DDP of bovine and human origin (Bakhtiar et al . 2020, Li et al . 2020), the present study demonstrates the benefit of using DDP in its intact form for dental pulp regeneration via stem cell transplantation where the retained vasculature could facilitate vascularization.…”

Section: Discussionmentioning

confidence: 99%

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Investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells: in vitro and preliminary in vivo evaluations

et al. 2021

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells: in vitro and preliminary in vivo evaluations

et al. 2021

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“…(1) naturally derived polymeric scaffolds like collagen, fibrin, decellularized dental pulp tissue (Alqahtani et al, 2018;Bakhtiar et al, 2020), and treated dentin matrix (TDM) (Yang et al, 2012;Meng et al, 2020); (2) synthetically engineered polymeric scaffolds such as polylactic acid, polyglycolic acid, and ceramic scaffolds; and (3) composite scaffolds that balance the advantages and disadvantages of individual material, improving the overall material performance (Moussa and Aparicio, 2019).…”

Section: Scaffold Materials Applicationmentioning

confidence: 99%

Microenvironment Influences Odontogenic Mesenchymal Stem Cells Mediated Dental Pulp Regeneration

Huang

Liu

et al. 2021

Front. Physiol.

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Dental pulp as a source of nutrition for the whole tooth is vulnerable to trauma and bacterial invasion, which causes irreversible pulpitis and pulp necrosis. Dental pulp regeneration is a valuable method of restoring the viability of the dental pulp and even the whole tooth. Odontogenic mesenchymal stem cells (MSCs) residing in the dental pulp environment have been widely used in dental pulp regeneration because of their immense potential to regenerate pulp-like tissue. Furthermore, the regenerative abilities of odontogenic MSCs are easily affected by the microenvironment in which they reside. The natural environment of the dental pulp has been proven to be capable of regulating odontogenic MSC homeostasis, proliferation, and differentiation. Therefore, various approaches have been applied to mimic the natural dental pulp environment to optimize the efficacy of pulp regeneration. In addition, odontogenic MSC aggregates/spheroids similar to the natural dental pulp environment have been shown to regenerate well-organized dental pulp both in preclinical and clinical trials. In this review, we summarize recent progress in odontogenic MSC-mediated pulp regeneration and focus on the effect of the microenvironment surrounding odontogenic MSCs in the achievement of dental pulp regeneration.

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“…traditional platelet-rich plasma 43 . In addition, pulp-derived ECM used in a 3D injectable scaffold has shown potential in regenerative therapy 44 . In fact, decellularized human dental pulp itself has been shown to support the proliferation and differentiation of SCAPs 12 .…”

Section: Host-derived Scaffoldsmentioning

confidence: 99%

Scaffold-based and Scaffold-free Strategies in Dental Pulp Regeneration

Dissanayaka

Zhang

2020

Journal of Endodontics

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Regenerative dentistry has come a long way from pulp capping to pulp regeneration research, which aims to regenerate the pulp-dentin complex and restore its functions compromised by pulp injury and/or inflammation. Because of unique anatomic limitations of the tooth structure, engineering a suitable microenvironment that facilitates angio/vasculogenesis and innervation is a challenging task. Cell-based tissue engineering approaches have shown great potential in achieving this goal. Biomedical approaches in creating a regenerative microenvironment are mainly represented by either scaffold-based or scaffold-free strategies. The scaffold-based strategy mainly relies on the use of biomaterials to create a structural base that supports cells throughout the process of tissue formation. The scaffold could be a classic 3-dimensional construct with interconnected pores, a hydrogel with cells embedded in it, or a combination of these 2. The scaffold-free approach has been considered a bottom-up strategy that uses cell sheets, spheroids, or tissue strands as building blocks. The outcome of this strategy relies on the capacity of these building blocks to secrete a favorable extracellular matrix and to fuse into larger tissue constructs. Both the scaffold-free and scaffold-based systems are required as complementary, rather than competing, approaches for pulp regeneration. A combined synergetic strategy, through which multicellular building blocks could be integrated with robust 3-dimensional scaffolds, might represent an optimal solution to circumvent some of the major drawbacks of the current methods in pulp regeneration while concurrently fostering their advantages.

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Pulp ECM-derived macroporous scaffolds for stimulation of dental-pulp regeneration process

Cited by 42 publications

References 23 publications

Investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells: in vitro and preliminary in vivo evaluations

Investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells: in vitro and preliminary in vivo evaluations

Microenvironment Influences Odontogenic Mesenchymal Stem Cells Mediated Dental Pulp Regeneration

Scaffold-based and Scaffold-free Strategies in Dental Pulp Regeneration

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