Pulmonary vascular morphology in a fetal rabbit model for congenital diaphragmatic hernia

“…The role of vascular smooth muscle cells appears to be crucial [77]. The physiologic reduction in medial thickness when approaching full term was not observed in CDH lungs [76][77][78]. Pulmonary vascular abnormalities in CDH consist of decreased number of pulmonary arteries per unit lung volume and an increase in the medial, adventitial and consequently total wall thickness of all arteries [4,45,[76][77][78][79][80][81].…”

Section: Pulmonary Vasculaturementioning

confidence: 99%

“…Pulmonary vascular abnormalities in CDH consist of decreased number of pulmonary arteries per unit lung volume and an increase in the medial, adventitial and consequently total wall thickness of all arteries [4,45,[76][77][78][79][80][81]. Due to failure of vascular remodeling, peripheral muscularization of pulmonary arterioles resulted in particularly medial and adventitial thickening [15,78,79,81]. The arterioles are predominantly responsible for pulmonary vascular resistance [15,79].…”

Section: Pulmonary Vasculaturementioning

confidence: 99%

The long-term follow-up of patients with a congenital diaphragmatic hernia: a broad spectrum of morbidity

et al. 2008

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Congenital diaphragmatic hernia (CDH) is a life-threatening anomaly with a mortality rate of approximately 40-50%, depending on case selection. It has been suggested that new therapeutic modalities such as nitric oxide (NO), high frequency oxygenation (HFO) and extracorporal membrane oxygenation (ECMO) might decrease mortality associated with pulmonary hypertension and the sequelae of artificial ventilation. When these new therapies indeed prove to be beneficial, a larger number of children with severe forms of CDH might survive, resulting in an increase of CDH-associated complications and/or consequences. In follow-up studies of infants born with CDH, many complications including pulmonary damage, cardiovascular disease, gastro-intestinal disease, failure to thrive, neurocognitive defects and musculoskeletal abnormalities have been described. Long-term pulmonary morbidity in CDH consists of obstructive and restrictive lung function impairments due to altered lung structure and prolonged ventilatory support. CDH has also been associated with persistent pulmonary vascular abnormalities, resulting in pulmonary hypertension in the neonatal period.

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“…Akciğer hipoplazisini geri çevirmeyi hedefleyen trakeal oklüzyon fikri buradan ortaya çıkmıştır. Literatürde hayvan modellerinde intrauterin dönemde gerçekleştirilen trakeal oklüzyonun akciğer hipoplazisini morfolojik olarak geri çevirdiğini ve postnatal dönemde akciğer fonksiyonlarını ve akciğer kompliansını arttırdığını gösteren çalışmalar mevcuttur (5)(6)(7)(8)(9)(10). Çalışmamızda trakeal oklüzyona ek olarak büyüme faktörleri, steroid ve surfaktan gibi akciğer gelişiminde rol oynayan etkenleri bir araya getirmeyi amaçladık.…”

Section: Introductionunclassified

To Analyse Effect of Tracheal Occlusion in the Development of Lung in Nitrofen Induced Congential Diaphragmatic Hernia Model

Aydın¹,

Yener²,

Üstündağ³

2016

Haseki

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ÖzAmaç: Doğumsal diyafragma hernisi yüksek morbidite ve mortaliteye sahip doğumsal anomalidir. Çalışmamızda nitrofen modeli ile oluşturulmuş doğumsal diyafragma hernili olgularda trakeal oklüzyonun akciğerlerin gelişimindeki etkisini incelemek amaçlanmaktadır. Yöntemler: Aynı yaş grubunda Wistar albino türü sıçanlar çiftleştirildi. Vajinal smearda sperm gösterilen sıçanlar çalışmaya dahil edildi. Smearda spermin gösterildiği gün sıfırıncı gebelik günü kabul edildi. Gebeliğin dokuzuncu gününde anne sıçana 100 mg nitrofen gavaj verildi ve 18. günde laparotomi ve histerotomi yapıldı. Her iki fetüsten birine servikal yaklaşımla trakeal oklüzyon uygulanırken diğerine trakeal oklüzyon yapılmadı. Gebe sıçanlara 22. gebelik gününde yeniden laparotomi ve histerotomi yapılarak fetüslerin akciğerleri çıkarıldı. Akciğerlerinin gelişimi histopatolojik olarak değerlendirildi. Bulgular: Nitrofen modeli ile 17 fetüsün 10'unda (%59) doğumsal diyafragma hernisi gelişti. Deneklerin beşi kontrol grubunda, beşi trakeal oklüzyon grubunda idi. Beş denekte sağ diyafragma hernisi, üç denekte sol diyafragma hernisi gerçekleşirken iki denekte herni bilateraldi. Akciğer gelişimi kontrol grubunda iki denekte psödoglandüler evrede, iki denekte psödoglandüler evre ve erken kanaliküler evrede, bir denekte ise erken kanaliküler evrede duraklamışken; trakeal oklüzyon grubunda iki denekte geç kanaliküler-erken sakküler evrede, bir denekte sakküler ve iki denekte erken kanaliküler evrede akciğer gelişiminin durakladığı görüldü. Sonuç: Nitrofen modeli doğumsal diyafragma hernisi modeli oluşturmada etkin bir yöntemdir ancak Bochdalek hernisini simüle etmede yetersiz kalmaktadır. Trakeal oklüzyon deneklerin akciğerlerinin gelişimlerini arttırmaktadır. Anahtar Sözcükler: Doğumsal diyafragmatik herni, 2,4 diklorofenil-p -nitrofenil eter, trakeal oklüzyon Aim:Congenital daphragmatic hernia is a congenital abnormality with high morbidity and mortality. In our study, we aimed to analyse the effect of tracheal occlusion in lung development in nitrofen-induced congenital daphragmatic hernia. Method:Wistar albino rats of the same age were mated. The rats, in whom the presence of sperm in vaginal smear was observed, were included in the study. It was zero day of pregnancy. At the ninth day of pregnancy, 100 mg nitrofen was introduced to pregnant rats by gavage and at the 18 th day of pregnancy, laparotomy and hysterotomy were performed. Tracheal occlusion was performed in one of two fetuses. At the 22 nd day of pregnancy, the lungs of the fetuses were excised. Lung development was analysed histopathologically.Results:Ten (59%) of 17 fetuses had congenital daphragmatic hernia. Five of them were in control group. The defect was right-sided in five of the subjects, left-sided in three, and bilateral in two. Lung development was observed to be stopped in pseudoglandular subjects in two of the fetuses, in pseudoglandular-early canalicular stage in two, and early canalicular in one of the fetuses in control group while late canalicularearly saccular in two, saccula...

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Pulmonary vascular morphology in a fetal rabbit model for congenital diaphragmatic hernia

Cited by 64 publications

References 27 publications

Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of congenital Diaphragmatic Hernia

Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of congenital Diaphragmatic Hernia

The long-term follow-up of patients with a congenital diaphragmatic hernia: a broad spectrum of morbidity

To Analyse Effect of Tracheal Occlusion in the Development of Lung in Nitrofen Induced Congential Diaphragmatic Hernia Model

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