Pulmonary toxicity of indium arsenide and arsenic selenide following repeated intratracheal instillations to the lungs of hamsters

Abstract

Chronic toxicity of indium arsenide (InAs) and arsenic selenide (As,Se,) was studied in male Syrian golden hamsters which received InAs or As,Se, particles, each containing a total dose of 7.5 mg of arsenic, by intratracheal instillations once a week for 15 weeks. As a control, hamsters were treated with the vehicle, phosphate buffer solution. During their total lifespan, the cumulative body weight gain of the hamsters in the InAs group was suppressed significantly compared with that in the control group, but not in the A%Se, group when compared with that in the control group. However, the survival rate for the InAs group was significantly higher compared with the control group, but not for the As,Se3 group when compared with the control group. During the animals' total lifespan, one lung adenoma was seen in the 27 hamsters in the InAs group and one lung adenoma in the 23 hamsters in the control group.No tumors of the lung were observed in the As,Se, group. Malignant tumors outside the lung appeared in four hamsters in the InAs group and in two in the As,Se, group. No non-lung malignant tumours were seen in the control group. Total tumor incidence rates were 25.9% (7/27) in the InAs group, 10.3% (3/29) in the A%Se, group and 8.7% (2/23) in the control group. There were therefore no significant differences in tumor incidence between the InAs or the Asae, group, and the control group.Regarding histopathological findings in the lung, incidence rates of proteinosis-like lesions, pneumonia, metaplastic ossification and emphysema were seen only in the InAs group, and alveolar or bronchiolar cell hyperplasia observed in both the InAs and the Asae, groups were at significantly higher rates than those in the control group.From these results, it was concluded that InAs and A%Se, particles could induce pulmonary toxicity when instilled intratracheally into hamsters. A great deal of attention should be paid to the toxicity of both InAs and Asfie,, even though in this study the adverse health effects of As,Se, appeared to be less than those of InAs.

Pulmonary Squamous Cyst Induced by Exposure to Indium Arsenide in Hamsters

“…In our previous study, we confirmed the development of proteinosis-like lesions or localized hyperplastic lesions in the lungs of hamsters which had been induced by exposure to InAs particles [3][4][5] . In particular, alveolar or bronchiolar cell hyperplasia with squamous cell metaplasia, squamous cell hyperplasia or squamous cell metaplasia with keratinization were observed when 7.7 mg/kg of InAs was instilled intratracheally, twice a week for a total of 14 times 4) .…”

“…In particular, alveolar or bronchiolar cell hyperplasia with squamous cell metaplasia, squamous cell hyperplasia or squamous cell metaplasia with keratinization were observed when 7.7 mg/kg of InAs was instilled intratracheally, twice a week for a total of 14 times 4) . Accordingly, a further study was undertaken with only half the dose (4 mg/kg).…”

Chronic Pulmonary Toxicity Study of Indium‐Tin Oxide and Indium Oxide Following Intratracheal Instillations into the Lungs of Hamsters

“…Due to the increasing production of ITO, the potential health hazards of occupational or environmental exposure to this material have began to cause great concern. Since the mid 1990's, data have become available indicating that indium compounds can be toxic to animals [2][3][4][5][6][7][8][9][10][11][12] , although there is little information regarding the adverse health effects for workers exposed to indium compounds. In 2003, the first case of interstitial pneumonia caused by occupational exposure to ITO was reported 13) .…”

Biological Monitoring of Indium by Means of Graphite Furnace Atomic Absorption Spectrophotometry in Workers Exposed to Particles of Indium Compounds

“…From the mid-1990s, animal experiments have shown that the inhalation or intratracheal instillation of In compounds causes severe lung inflammation [1][2][3][4][5][6][7] and mild adverse reproductive effects [8][9][10] . Although the health effects of In on workers' respiratory and reproductive systems have been unclear to date, assessing the exposure-effect relationships in Inexposed workers is a serious concern, but no information is available on the exposure-effect relationships in workers.…”