Pulmonary toxicity induced by three forms of titanium dioxide nanoparticles via intra-tracheal instillation in rats

Liu, Ran; Yin, Lihong; Pu, Yuepu; Liang, Geyu; Zhang, Juan; Su, Yaoyao; Zhang, Xiao; Yan, Bing

doi:10.1016/j.pnsc.2008.06.020

Cited by 78 publications

(37 citation statements)

References 26 publications

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“…Our results are consistent with those of others, who have demonstrated the cytotoxic effect of TiO 2 NPs in different cell lines and in vivo. 4,[22][23][24] Based on all these data, 48 hours was chosen as the incubation time for further biochemical study.…”

Section: 16mentioning

confidence: 99%

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

Niska¹,

Pyszka²,

Tukaj³

et al. 2015

IJN

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Titanium dioxide (TiO 2 ) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO 2 NPs are frequently used as a material for orthopedic implants. However, to the best of our knowledge, the biocompatibility of TiO 2 NPs and their effects on osteoblast cells, which are responsible for the growth and remodeling of the human skeleton, have not been thoroughly investigated. In the research reported here, we studied the effects of exposing hFOB 1.19 human osteoblast cells to TiO 2 NPs (5–15 nm) for 24 and 48 hours. Cell viability, alkaline phosphatase (ALP) activity, cellular uptake of NPs, cell morphology, superoxide anion (O 2 •−2 ) generation, superoxide dismutase (SOD) activity and protein level, sirtuin 3 (SIR3) protein level, correlation between manganese (Mn) SOD and SIR, total antioxidant capacity, and malondialdehyde were measured following exposure of hFOB 1.19 cells to TiO 2 NPs. Exposure of hFOB 1.19 cells to TiO 2 NPs resulted in: (1) cellular uptake of NPs; (2) increased cytotoxicity and cell death in a time- and concentration-dependent manner; (3) ultrastructure changes; (4) decreased SOD and ALP activity; (5) decreased protein levels of SOD1, SOD2, and SIR3; (6) decreased total antioxidant capacity; (7) increased O 2 •− generation; and (8) enhanced lipid peroxidation (malondialdehyde level). The linear relationship between the protein level of MnSOD and SIR3 and between O 2 •− content and SIR3 protein level was observed. Importantly, the cytotoxic effects of TiO 2 NPs were attenuated by the pretreatment of hFOB 1.19 cells with SOD, indicating the significant role of O 2 •− in the cell damage and death observed. Thus, decreased expression of SOD leading to increased oxidizing stress may underlie the nanotoxic effects of TiO 2 NPs on human osteoblasts.

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Section: 16mentioning

confidence: 99%

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

Niska¹,

Pyszka²,

Tukaj³

et al. 2015

IJN

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“…Dose-dependent alveolar proteinosis, collagenized fibrosis, thickened alveolar walls impairing oxygen diffusion, cholesterol granuloma formation, and focal pleurisy have been observed in rats exposed to TNPs [40,105].…”

Section: Evaluation Model and Experimental Conditionsmentioning

confidence: 99%

The potential health risk of titania nanoparticles

Zhang

Bai

Zhang

et al. 2012

Journal of Hazardous Materials

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“…Studies had shown that by intra-tracheal instillation of TiO 2 nanoparticles in rats, pulmonary inflammation and cytotoxicity is observed [20][21][22]. TiO 2 nanoparticles with oral gavages increased the activity of lactate dehydrogenase causing hepatocyte necrosis in mice [23]. Intraperitoneal injection of various doses of TiO 2 nanoparticles can lead to its accumulation in the mouse spleen [9].…”

Section: Introductionmentioning

confidence: 99%

Aqueous Synthesis and Concentration-Dependent Dermal Toxicity of TiO2 Nanoparticles in Wistar Rats

Unnithan

Rehman

Ahmad

et al. 2011

Biol Trace Elem Res

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A number of dermal toxicological studies using TiO(2) nanoparticles exist which are based on the study of various animal models like mice, rabbits etc. However, a well-defined study is lacking on the dermal toxic effects of TiO(2) nanoparticles on rats, which are the appropriate model for systemic absorption study of nanoparticles. Furthermore, toxicity of TiO(2) nanoparticles varies widely depending upon the size, concentration, crystallinity, synthesis method etc. This study was conducted to synthesize TiO(2) nanoparticles of different sizes (∼15 to ∼30 nm) by aqueous method, thereby evaluating the concentration-dependent toxicological effects of the ∼20-nm sized nanoparticles on Wistar rats. Characterization of the particles was done by transmission electron microscope, dynamic light scattering instrument, X-ray diffractrometer, and ultraviolet spectrophotometer. The toxicity study was conducted for 14 days (acute), and it is observed that TiO(2) nanoparticles (∼20 nm) at a concentration of 42 mg/kg, when applied topically showed toxicity on rat skin at the biochemical level. However, the histopathological studies did not show any observable effects at tissue level. Our data suggest that well-crystallized spherical-shaped ∼20 nm anatase TiO(2) nanoparticles synthesized in aqueous medium can induce concentration-dependent biochemical alteration in rat skin during short-term exposure.

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Pulmonary toxicity induced by three forms of titanium dioxide nanoparticles via intra-tracheal instillation in rats

Cited by 78 publications

References 26 publications

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

The potential health risk of titania nanoparticles

Aqueous Synthesis and Concentration-Dependent Dermal Toxicity of TiO2 Nanoparticles in Wistar Rats

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