Pulmonary NAD+-linked 15-hydroxyprostaglandin dehydrogenase activity is decreased by cigarette smoking

Chang, Wen Chang; Fukuda, Shoshi; Tai, Hsin Hsiung

doi:10.1016/0024-3205(84)90549-6

Cited by 10 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In view of the rapid turnover of 15-PGDH, enzyme activity will depend on continued enzyme synthesis, and this could be very susceptible to hormonal and drug control mechanisms. Several reports have demonstrated alterations in 1 5-PGDH activity caused by hormonal changes [8], steroid administration [11], endotoxin shock [35] or cigarette smoking [36] and in tissues from spontaneously hypertensive rats [37]. Although alteration of these enzyme activities may be the consequence of post-translational modifications of 15-PGDH, such as the phosphorylation suggested above, changes in enzyme synthesis and content are also very likely possibilities.…”

Section: Molecular Mass (Kda)mentioning

confidence: 99%

Stimulation of synthesis de novo of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase in human promyelocytic leukaemia (HL-60) cells by phorbol ester

Xun

Tian

Tai

1991

Biochemical Journal

View full text Add to dashboard Cite

Human promyelocytic leukaemia (HL-60) cells were employed to study the induction of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH), the key enzyme in controlling prostaglandin inactivation. Phorbol 12-myristate 13-acetate (PMA) stimulated 15-PGDH activity in a time- and concentration-dependent manner. Dimethyl sulphoxide (DMSO) also stimulated the enzyme activity, although a much delayed stimulation was observed. Western blot studies indicated that PMA increased significantly a 28 kDa immunoreactive protein characteristic of 15-PGDH. L-[35S]Methionine labelling of the PMA-treated cells showed a similar enhancement over the control cells. These studies indicate that PMA induced synthesis of 15-PGDH. Stimulation of 15-PGDH activity by PMA or DMSO appears to be mediated by protein kinase C activation, since an inactive analogue of PMA failed to induce the effect, and both staurosporine and H-7 blocked the stimulation. Stimulation by PMA was optimal at 10 nM and less effective at higher concentrations. Western blot studies indicated that a similar, if not greater, amount of enzyme protein was induced at high concentrations of PMA, suggesting that enzyme inactivation might be occurring. Possible enzyme inactivation by protein kinase C activation was further examined by incubating DMSO-treated cells with a high concentration of PMA (50 nM). Time-dependent inactivation of 15-PGDH within the first 1 h was observed and this inactivation was partially blocked by staurosporine and H-7. Pulse-chase experiments indicated that 15-PGDH had a rapid turnover rate (t 1/2 = 47 min), and PMA shortened the half-life of the enzyme (t 1/2 = 33 min), suggesting that PMA might have an additional effect on 15-PGDH degradation. The rapid turnover of 15-PGDH indicates that the enzyme activity depends on continued enzyme synthesis, and this could be susceptible to hormone and drug control mechanisms.

show abstract