Pulmonary mechanic and lung histology injury induced by Crotalus durissus terrificus snake venom

Nonaka, Paula Naomi; Amorim, César Ferreira; Peres, Ana Claudia Paneque; Silva, César Augusto Melo e; Zamunér, Stella Regina; Ribeiro, Wellington; Cogo, José Carlos; Dolhnikoff, Marisa; Oliveira, Cláudia Santos

doi:10.1016/j.toxicon.2008.02.006

Cited by 14 publications

(21 citation statements)

References 22 publications

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“…However, the doses of 300 and 700 μg·kg −1 did not produce significant changes in any of the evaluated metabolic parameters. This range of doses, between 300 and 1,500 μg·kg −1 , is compatible with those used in studies performed in mice [33] and dogs [34] but is lower than the dose previously used in rats (2.0 mg·kg −1 ) [35]. The absence of significant differences in the liver metabolism with both lowest venom doses indicates that (a) the aminotransferase release, lipoperoxidation, and enzymes related to oxidative stress are more sensitive to the venom than the metabolic pathways; and (b) an overdose of venom is necessary to cause significant metabolic modifications.…”

Section: Discussionsupporting

confidence: 70%

Effects of the crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress

Silva

Soley

Gris

et al. 2010

J Biochem & Molecular Tox

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Snake venoms present different action mechanisms because of their complex composition, represented mainly by toxins and enzymes. This work aimed to investigate the effects of the Crotalus durissus terrificus(Cdt) venom in the liver. Wistar rats were inoculated intraperitoneally with saline (control) or Cdt venom. After 3, 4, or 6 h, the following parameters were analyzed: (a) hepatic function, (b) oxidative stress parameters, and (c) the metabolism of alanine in the isolated perfused liver. Plasma activities of alanine aminotransferase and aspartate aminotransferase and hepatic glutathione S-transferase and catalase presented significant elevation in rats inoculated with 300 μg ⋅ kg(-1) Cdt venom. Liver lipoperoxidation was enormously increased by venom doses of 100, 200, and 300 μg ⋅kg(-1) , whereas glutathione S-transferase was not changed. Perfused livers from rats inoculated with 1500 μg ⋅kg(-1) venom showed increased production of lactate, pyruvate, and ammonia when alanine was the metabolic substrate. These results demonstrate that the Cdt venom can produce several changes in hepatocytes. The causes of the changes are possibly related to the disequilibrium in the redox homeostasis but also to specific needs of the poisoned organism, for example, an increased supply of lactate and pyruvate in response to an increased activity of the Cori cycle.

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Section: Discussionsupporting

confidence: 70%

Effects of the crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress

Silva

Soley

Gris

et al. 2010

J Biochem & Molecular Tox

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“…In a subsequent step, effective lung resistance (R L ) and elastance (E L ) were computed by linear regression fitting of the recorded signals P tr , V′, and V to the conventional respiratory mechanics model P tr = P o + E L × V + R L × V′, where P o is a parameter to account for the external positive end-expiratory pressure applied by the ventilator. For each animal, R L and E L were computed from data obtained during 5 breathing cycles [21, 22]. …”

Section: Methodsmentioning

confidence: 99%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 × 10⁴ colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1β (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-α (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-κB expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Pre-incubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-α (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/NF-κB, and redox signaling.

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“…These dysfunctions and local wounds are specific clinical symptoms of B. neuwiedi envenomation (17). Several myotoxins have been isolated from Bothrops snake venoms and their effects appear to be similar to other toxins found in crotalic venoms, such as Crotalus durissus terrificus (23). Intramuscular injection of Bothrops myotoxins leads to a rapid series of drastic degenerative events, probably initiated at the plasma membrane level, that culminate in selective skeletal muscle necrosis.…”

Section: Introductionmentioning

confidence: 98%

The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom

Dourado¹,

Matias²,

Almeida³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:The present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n = 24 each): Group S (0.9% saline solution); Group V (venom) and Group VLLL (venom plus low-level laser). These categories were subdivided into four additional groups (n = 6) based on the euthanasia timing (3 hours, 24 hours, 3 days and 7 days). The groups V and VLLL were inoculated with 100 µL of concentrated venom (40 µg/mL) in the gastrocnemius muscle. The muscle was irradiated using a gallium-aluminumarsenide laser (GaAlAs) at 35 mW power and 4 J/cm² energy density for 3 hours, 24 hours, 3 days or 7 days after venom inoculation. To evaluate the myotoxic activity of the venom, CK activity was measured and the muscle was histologically analyzed. The low-level laser reduced venom-induced CK activity in the groups euthanized at 3 hours, 24 hours and 3 days (p < 0.0001). Histological analysis revealed that low-level laser reduced neutrophilic inflammation as well as myofibrillar edema, hemorrhage and myonecrosis following B. neuwiedi envenomation. These results suggest that low-level laser can be useful as an adjunct therapy following B. neuwiedi envenomation.

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Pulmonary mechanic and lung histology injury induced by Crotalus durissus terrificus snake venom

Cited by 14 publications

References 22 publications

Effects of the crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress

Effects of the crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom

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