Pulmonary Inflammatory, Chemokine, and Mutagenic Responses in Rats after Subchronic Inhalation of Carbon Black

Driscoll, Kevin E.; Carter, Janet M.; Howard, Brian W.; Hassenbein, Diana G.; Pepelko, William E.; Baggs, Raymond B.; Oberdörster, Günter

doi:10.1006/taap.1996.0045

Cited by 212 publications

(145 citation statements)

References 0 publications

Supporting

Mentioning

128

Contrasting

Unclassified

Order By: Relevance

“…Corundum, a noncarcinogenic particle, was not harmful to the rat lung. These observations support the suggestion that inflammation, in conjunction with the persistence of mutagenic DNA oxidation products (e.g., 8-oxoGua) in target cells and an increased proliferation of target cells, plays an important role in the particle-induced pathogenesis of rat lung tumors. It is not known how long 8-oxoGua levels in target cells must be elevated until the multistep process of carcinogenesis is initiated and completed.…”

Section: Discussionsupporting

confidence: 86%

“…Recent in vitro and in vivo studies demonstrated that the mutagenic and tumorigenic effects of particles and fibers in the lung are closely linked to the formation of ROS (4)(5)(6)(7)(8). ROS generate different types of DNA modifications, including single strand breaks in the DNA double helix, 8-oxoguanine (8-oxoGua), the most frequently occurring mutagenic base modification, and various other mutagenic DNA oxidation products (9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Formation and persistence of 8-oxoguanine in rat lung cells as an important determinant for tumor formation following particle exposure.

Nehls

Seiler

Rehn

et al. 1997

Environ Health Perspect

View full text Add to dashboard Cite

Exposure of rats to quartz (or various other particles) can lead to the development of lung tumors. At the moment, the mechanisms involved in particle-induced tumor formation are not clarified. However, it is suggested that inflammation, in conjunction with the production of reactive oxygen species (ROS) and an enhancement of epithelial cell proliferation, may play a key role in the development of lung tumors. ROS induces 8-oxoguanine (8-oxoGua) and other mutagenic DNA oxidation products, which can be converted to mutations in proliferating cells. Mutation formation in cancer-related genes is a critical event with respect to tumor formation. In this study we investigated the effects of quartz (DQ1 2) and of the nontumorigenic dust corundum on the induction of 8-oxoGua in the DNA of rat lung cells, as well as on cell proliferation and pulmonary inflammation. Wistar rats were exposed by intratracheal instillation to quartz (2.5 mg/rat) or corundum (2.5 mg/rat) suspended in physiological saline; control animals exposed to physiological saline or left untreated. Measurements were carried out 7, 21, and 90 days after the exposures. 8-oxoGua levels were determined in lung tissue sections at the single cell level by immunocytological assay using a rabbit anti-8-oxoGua antibody. After exposure to quartz, 8-oxoGua levels were significantly increased at all time points of investigation. Additionally, we observed inflammation and an enhanced cell proliferation. Exposure to corundum had no adverse effects on the lung; neither increased 8-oxoGua levels nor enhanced cell proliferation or inflammation were detected. These observations support the suggestion that inflammation associated with increased 8-oxoGua levels in lung cells and increased cell proliferation is an important determinant for particle-induced development of lung tumors in the rat.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Formation and persistence of 8-oxoguanine in rat lung cells as an important determinant for tumor formation following particle exposure.

Nehls

Seiler

Rehn

et al. 1997

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…Atmospheric black carbon (BC) is an important indicator of combustion emissions in ambient or indoor environments, may have direct impacts on health (e.g., Driscoll et al, 1996;Stoeger et al, 2006), and has been highlighted as a significant forcing agent for climate change (e.g., Ramanathan and Carmichael, 2008). Field studies around the world quantify atmospheric black carbon through two general approaches -off-line measurements where a sample is collected onto a filter and then measured in a laboratory setting; and online measurements where BC is continuously measured and reported on a time base of seconds to minutes.…”

Section: Introductionmentioning

confidence: 99%

Post-processing Method to Reduce Noise while Preserving High Time Resolution in Aethalometer Real-time Black Carbon Data

Hagler

Vedantham

Turner

2011

Aerosol Air Qual. Res.

207

123

View full text Add to dashboard Cite

Real-time aerosol black carbon (BC) data, presented at time resolutions on the order of seconds to minutes, is desirable in field and source characterization studies measuring rapidly varying concentrations of BC. The Optimized Noisereduction Averaging (ONA) algorithm has been developed to post-process data from the Aethalometer, one of the widely used real-time BC instruments. The ONA program conducts adaptive time-averaging of the BC data, with the incremental light attenuation (∆ATN) through the instrument's internal filter determining the time window of averaging. Analysis of instrument noise and the algorithm performance was conducted using Aethalometer 1-second data from a soot generation experiment, where input BC concentrations were maintained constant and an optimal ∆ATN min value was defined. The ONA procedure was applied to four additional data sets (1 s to 5 min data), including cookstove emissions tests, mobile monitoring, continuous near-road measurements, and indoor air sampling. For these data, the algorithm reduces the occurrence of negative values to virtually zero while preserving the significant dynamic trends in the time series.

show abstract

“…Several cell types have been used to better elucidate the specific actions of PS. Studies with cancer cells have demonstrated that PS may reduce (22) or inhibit (23) cell proliferation, its effect being dependent on dose and cell type. In vitro PS inhibits the proliferation of smooth muscle cells and the formation of arterial plaque, thus highlighting their antiproliferative action, with no evidence of cytotoxicity (24).…”

Section: Studies On Cellsmentioning

confidence: 99%

Do clinical and experimental investigations support an antiatherogenic role for dietary phytosterols/stanols?

et al. 2012

View full text Add to dashboard Cite

SummaryThe plasma cholesterol-reducing effect of phytosterols (PS) has been recognized in several studies, but the usefulness of PS in preventing coronary heart disease remains controversial, as some investigations claim that the high PS concentrations found in plasma and specific tissues are related to an increased risk of cardiovascular events. It has also been demonstrated that PS may induce inflammation and reduce cholesterol efflux from macrophages, conditions that are directly implicated in the development of atherosclerosis. As to arterial dysfunction and atherosclerosis, some studies have concluded that plasma PS concentrations are unrelated or only weakly related or that PS intake or plasma PS concentrations are harmful. Thus, in light of the National Cholesterol Education Program-ATPIII report, it is necessary to evaluate the relevance of their findings. To this end, we have evaluated the studies conducted on cells, animal models, and humans regarding the influence of PS on the development of atherosclerosis.2012 IUBMB IUBMB Life, 64(4): [296][297][298][299][300][301][302][303][304][305][306] 2012

show abstract

Pulmonary Inflammatory, Chemokine, and Mutagenic Responses in Rats after Subchronic Inhalation of Carbon Black

Cited by 212 publications

References 0 publications

Formation and persistence of 8-oxoguanine in rat lung cells as an important determinant for tumor formation following particle exposure.

Formation and persistence of 8-oxoguanine in rat lung cells as an important determinant for tumor formation following particle exposure.

Post-processing Method to Reduce Noise while Preserving High Time Resolution in Aethalometer Real-time Black Carbon Data

Do clinical and experimental investigations support an antiatherogenic role for dietary phytosterols/stanols?

Contact Info

Product

Resources

About