Pulmonary infection due to Pseudozyma aphidis in a patient with burkitt lymphoma: first case report

Parahym, Ana Maria Rabelo de Carvalho; Silva, Carolina Maria da; Domingos, Igor de Farias; Gonçalves, Sarah Santos; Rodrigues, Márcia de Melo; Morais, Vera Lúcia Lins de; Neves, Rejane Pereira

doi:10.1016/j.diagmicrobio.2012.09.010

Cited by 24 publications

(22 citation statements)

References 13 publications

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“…However, on CHROMagar Candida, our isolates developed creamcolored colonies, in contrast with the findings of Prakash et al (14), and could not be identified using an API 20 AUX system. Due to this, sequencing of ITS and/or D1/D2 domains was necessary for proper identification, in agreement with the literature (8,(11)(12)(13)(14)(15). Also, isolates could not be identified by MALDI-TOF.…”

supporting

confidence: 70%

“…Unfortunately, we have no record of the patient dietary history. It is also worth noting that, as in the present work, three of the four cases in which P. aphidis were isolated involved pediatric patients (11,(13)(14)(15).…”

supporting

confidence: 69%

“…It is worthy of note, as mentioned before (14), that almost all cases of Pseudozyma infections are invasive, and it has been proposed that, in those cases, hand-related transmission (11), inoculation by needle biopsy (12), vaginal or nosocomial transmission during birth (14), inhalation of conidia from the environment (15), and translocation of fungus across mucosal gastrointestinal barriers in patients with compromised integrity and anatomy of the gastrointestinal tract (11) represent potential portals of entry for CVC contamination. Since Pseudozyma spp., as well as Ustilago spp., are associated with corn, dietary history is important in evaluating patients with invasive disease caused by these yeasts (11).…”

mentioning

confidence: 99%

“…Since then, other cases of human infection due to Pseudozyma species have been reported: a CVCassociated infection due to P. aphidis in a child with short gut syndrome (11), a brain abscess associated with a Pseudozyma sp. in a patient with astrocytoma (12), a case of mycetoma associated with Nocardia otitidiscaviarum and P. aphidis (13), a fungemia due to P. aphidis in a neonate (14), a pulmonary infection due to P. aphidis in a patient with Burkitt lymphoma (15), and a new report that shows the isolation of three new additional Pseudozyma species (Pseudozyma alboarmeniaca sp. nov., Pseudozyma crassa sp.…”

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confidence: 99%

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First Case of Fungemia Due to Pseudozyma aphidis in a Pediatric Patient with Osteosarcoma in Latin America

et al. 2015

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We report the first case of blood infection due to Pseudozyma aphidis in Latin America. We contribute evidence showing this organism to be a potential human pathogen, and we provide new data about its identification, drug susceptibility, and treatment outcome. CASE REPORTA 6-year-old female from Córdoba, Argentina, was diagnosed with osteosarcoma of the left tibia and lung metastasis and started chemotherapy administered through a long-term indwelling central line (a central venous catheter [CVC]) on 2 September 2013 at the Hospital de niños de la Santísima Trinidad. On 2 October, she presented with febrile neutropenia and was empirically treated with ceftazidime, vancomycin, and amikacin; blood and urine samples were submitted for laboratory testing, and both cultures were negative. On 4 October, she received clindamycin due to facial edema. From 6 October to 13 November, she was transfused with platelets and red blood cells several times due to thrombocytopenia and anemia. On 5 November, she developed severe mucositis and erythroderma and was treated with ceftazidime, nystatin, amikacin, vancomycin, and fluconazole. On 13 November, she received a 1-week treatment with meropenem and liposomal amphotericin B. On 18 November, she developed conjunctival hyperemia and was treated with tobramycin. On 28 November, she underwent supracondylar amputation of the left lower limb. On 20 December, the patient presented with febrile neutropenia (white blood cells [WBCs], 1,030/mm 3 ) and was admitted to the intensive care unit (ICU) in the same hospital. Table 1 summarizes the clinical data and evolution of the case since admission. A blood sample (BS1) obtained by venipuncture and a hub blood sample (drawn through the catheter hub) (HBS1) were taken and sent to the microbiology laboratory. Yeast cells (Pseudozyma aphidis; isolate Y1) were detected in the hub blood sample after 2.6 days of incubation in a Bact/ALERT PF (bioMérieux Inc., Durham, NC). However, the patient did not show clinical signs of illness on day 3 after admission; therefore, the isolate was considered not significant and she was discharged. Four days later, the patient presented with vomiting, dehydration, and febrile neutropenia (WBCs, 200/mm 3 ) and was admitted once again at the ICU in the same institution. At that point, an empirical antibiotic treatment with ceftazidime and amikacin was initiated and a new blood sample and hub blood sample (BS2 and HBS2) were taken and processed. After 18 h of incubation, both cultures were positive for Pseudomonas aeruginosa. Four days after readmission, the patient presented with fever and severe neutropenia (WBCs, 80/ mm 3 ) and showed high (233 mg/liter) C-reactive protein levels. A blood sample and a hub blood sample (BS3 and HBS3) were taken and processed. After 12 h of incubation, the blood sample culture was positive for Escherichia coli, and 2 h later, the hub blood sample culture was positive for yeast (Y2). This isolate (Y2) had the same phenotypic features as the first yeast isolate (Y1). Six days after...

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supporting

confidence: 70%

supporting

confidence: 69%

mentioning

confidence: 99%

mentioning

confidence: 99%

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First Case of Fungemia Due to Pseudozyma aphidis in a Pediatric Patient with Osteosarcoma in Latin America

et al. 2015

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“…infection most often presents with fungaemia. Moreover, deep-seated focal infections including brain abscess [125] and pleural cavity have been reported [124]. Finally, a single case of a mycetoma due to co-infection with Nocardia after traumatic inoculation in an Asian farmer has been described [126].…”

Section: Risk Factors/clinical Presentationmentioning

confidence: 99%

ESCMID and ECMM joint clinical guidelines for the diagnosis and management of rare invasive yeast infections

Arendrup

Boekhout

Akova

et al. 2014

Clinical Microbiology and Infection

422

396

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The mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than Candida being no exception. This is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging intrinsic susceptibility pattern of the pathogens that potentially leads to delayed appropriate antifungal treatment. A panel of experts of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study Group (EFISG) and the European Confederation of Medical Mycology (ECMM) undertook a data review and compiled guidelines for the diagnostic tests and procedures for detection and management of rare invasive yeast infections. The rare yeast pathogens were defined and limited to the following genera/species: Cryptococcus adeliensis, Cryptococcus albidus, Cryptococcus curvatus, Cryptococcus flavescens, Cryptococcus laurentii and Cryptococcus uniguttulatus (often published under the name Filobasidium uniguttulatum), Malassezia furfur, Malassezia globosa, Malassezia pachydermatis and Malassezia restricta, Pseudozyma spp., Rhodotorula glutinis, Rhodotorula minuta and Rhodotorula mucilaginosa, Sporobolomyces spp., Trichosporon asahii, Trichosporon asteroides, Trichosporon dermatis, Trichosporon inkin, Trichosporon jirovecii, Trichosporon loubieri, Trichosporon mucoides and Trichosporon mycotoxinivorans and ascomycetous ones: Geotrichum candidum, Kodamaea ohmeri, Saccharomyces cerevisiae (incl. S. boulardii) and Saprochaete capitatae (Magnusiomyces (Blastoschizomyces) capitatus formerly named Trichosporon capitatum or Geotrichum (Dipodascus) capitatum) and Saprochaete clavata. Recommendations about the microbiological investigation and detection of invasive infection were made and current knowledge on the most appropriate antifungal and supportive treatment was reviewed. In addition, remarks about antifungal susceptibility testing were made.

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Characterization of Hyaluronan-Degrading Enzymes from Yeasts

Smirnou

Krčmář

Kulhánek

et al. 2015

Appl Biochem Biotechnol

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Hyaluronidases (HAases) from yeasts were characterized for the first time. The study elucidated that hyaluronate 4-glycanohydrolase and hyaluronan (HA) lyase can be produced by yeasts. Six yeasts producing HAases were found through express screening of activities. The extracellular HAases from two of the yeast isolates, Pseudozyma aphidis and Cryptococcus laurentii, were characterized among them. P. aphidis HAase hydrolyzed β-1,4 glycosidic bonds of HA, yielding even-numbered oligosaccharides with N-acetyl-D-glucosamine at the reducing end. C. laurentii produced hyaluronan lyase, which cleaved β-1,4 glycosidic bonds of HA in β-elimination reaction, and the products of HA degradation were different-sized even-numbered oligosaccharides. The shortest detected HA oligomer was dimer. The enzymes' pH and temperature optima were pH 3.0 and 37-45 °C (P. aphidis) and pH 6.0 and 37 °C (C. laurentii), respectively. Both HAases showed good thermostability.

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Pulmonary infection due to Pseudozyma aphidis in a patient with burkitt lymphoma: first case report

Cited by 24 publications

References 13 publications

First Case of Fungemia Due to Pseudozyma aphidis in a Pediatric Patient with Osteosarcoma in Latin America

First Case of Fungemia Due to Pseudozyma aphidis in a Pediatric Patient with Osteosarcoma in Latin America

ESCMID and ECMM joint clinical guidelines for the diagnosis and management of rare invasive yeast infections

Characterization of Hyaluronan-Degrading Enzymes from Yeasts

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