Pulmonary Disease Diagnostic and Rehabilitation Treatment for Patients With Overlap Asthma and Chronic Obstructive Pulmonary Diseases

Sukhan, Victoria S.

doi:10.36740/merkur202304103

Cited by 1 publication

(1 citation statement)

References 27 publications

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“…Another group showed that HLA-A was significantly increased in the airway of patients with COPD, and its expression was decreased after intervention with azithromycin, suggesting that HLA-A may be involved in the 1632 antibiotic effects of azithromycin in the treatment of COPD. 21 Although there is currently a lack of research investigating the specific functions of HLA-A in COPD, it appears to warrant future research. Eosinophil cationic protein (ECP) is a secreted protein with potent cytotoxic, antibacterial, and antiviral properties.…”

Section: Discussionmentioning

confidence: 99%

Serum Proteomic Profiling in Patients with Chronic Obstructive Pulmonary Disease

Wu¹,

Huang²,

Chang³

et al. 2023

COPD

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Purpose Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease with high morbidity and mortality rates. This study used proteomic profiling of serum to identify the differentially expressed proteins in COPD patients compared with healthy controls, to expand the knowledge of COPD pathogenesis and to ascertain potential new targets for diagnosis and treatment of COPD. Methods Serum samples were collected from 56 participants (COPD group n = 28; Healthy Control group n = 28). A data-independent acquisition quantitative proteomics approach was used to identify differentially expressed proteins (DEPs) between the two groups. Gene Ontology (GO) functional annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway functional enrichment, and protein–protein interaction analyses of DEPs were conducted to identify their relevant biological processes, cellular components, and related pathways. We used a parallel reaction monitoring (PRM)-based targeted quantitative proteomics approach to validate those findings. Results Of 8484 peptides identified by searching the UniProtKB/Swiss-Prot knowledgebase, 867 proteins were quantifiable, of which 20 were upregulated and 35 were downregulated in the COPD group. GO functional annotation indicated that the subcellular localization of most DEPs was extracellular. The top three molecular functions of the DEPs were signaling receptor binding, antigen binding, and immunoglobulin receptor binding. The most relevant biological process was immune response. The transforming growth factor-β signaling pathway, Staphylococcus aureus infection, and hematopoietic cell lineage were the top three pathways identified in the KEGG pathway functional enrichment. Our PRM analyses confirmed the identification of 11 DEPs identified in our data-independent acquisition analyses, 8 DEPs were upregulated and 3 DEPs were downregulated. Conclusion This study using data-independent acquisition analyses with PRM confirmation of findings identified 11 DEPs in the serum of patients with COPD. These DEPs are potential diagnostic or prognostic biomarkers or may be future targets for the treatment of COPD.

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Section: Discussionmentioning

confidence: 99%