Pulmonary Blast Injury Increases Nitric Oxide Production, Disturbs Arginine Metabolism, and Alters the Plasma Free Amino Acid Pool in Rabbits during the Early Posttraumatic Period

Z̆unić, Gordana; Pavlović, Radmila; Malicevic, Zivorad; Savic, V.; Černak, Ibolja

doi:10.1006/niox.2000.0276

Cited by 29 publications

(12 citation statements)

References 25 publications

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“…In the present study we used ultrafiltration for protein removal, preventing sample acidification and concomitantly reducing artificial increase of GSSG. In addition the levels of examined aromatic amino acids in whole blood hydrolysates obtained in the present study are significantly higher from reported plasma values [12][13][14] confirming the fact that their intracellular levels are above extracellular ones. Recently, the others [23] have also reported CE as a suitable tool for the quality control of amino acids and glutathione, although they analyzed samples after derivatization and testing the method for analysis in the pharmaceutical field.…”

Section: Testing the Methods On Blood Sample Analysissupporting

confidence: 83%

“…Namely, free aromatic amino acids such as: histidine (His), tryptophane (Trp), phenylalanine (Phe) and tyrosine (Tyr) particularly reflect disturbance in protein anabolism/catabolism ratio in an organism [12,13]. Considering these findings it would be very useful to have method permitting simultaneous measurement of these amino acids and both forms of glutathione in the single run.…”

Section: Introductionmentioning

confidence: 99%

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Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood

Z̆unić

Spasić

2008

Journal of Chromatography B

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Section: Testing the Methods On Blood Sample Analysissupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood

Z̆unić

Spasić

2008

Journal of Chromatography B

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“…However, the effect of surgery and wound healing process after surgical trauma on amino acid metabolism and PFAA profile is still unclear. An animal study has shown that pulmonary blast injury induces prompt arginine elevation through NO synthase [ 43 ]. Moreover, a persistent drop of arginine, which contributes to T cell dysfunction and decrease of nitric oxide (NO) production, was found to significantly increase susceptibility to infections and organ failure after trauma or surgery.…”

Section: Discussionmentioning

confidence: 99%

Perioperative dynamics and significance of amino acid profiles in patients with cancer

Chen

et al. 2015

J Transl Med

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BackgroundMetabolome analysis including amino acid profile is under investigation as an approach in cancer screening. The present study aims to analyze plasma free amino acid (PFAA) profiles in cancer patients and investigate their potential as biomarkers of malignancy.MethodsPlasma samples from 56 gastric cancer patients, 28 breast cancer patients, 33 thyroid cancer patients, and 137 age-matched healthy controls were collected in the study. PFAA levels were measured and their perioperative alterations were analyzed. Biological effects of ten cancer-related amino acids were further validated in gastric and breast cancer cells.ResultsWe found that PFAA profiles of cancer patients differed significantly from those of healthy controls. Decreased concentrations of PFAAs were associated with lymph node metastases in gastric cancer. Levels of PFAAs such as aspartate and alanine increased after tumor resection. PFAA levels correlated with clinical tumor markers in gastric cancer patients and pathological immunohistochemistry markers in breast cancer patients. Specifically, alanine, arginine, aspartate and cysteine had proliferative effects on breast cancer cells. Proliferation of gastric cancer cells was promoted by cysteine, but inhibited by alanine and glutamic acid. Furthermore, alanine treatment decreased total and stable fraction of gastric cancer cells, and alanine and glutamic acid induced apoptosis of gastric cancer cells.ConclusionsPFAA patterns in cancer patients are altered perioperatively. Tumor-related amino acids identified by dynamic study of PFAA patterns may have the potential to be developed as novel biomarkers for diagnosis and prognosis of cancer patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s12967-015-0408-1) contains supplementary material, which is available to authorized users.

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“…Firstly, the hemodynamic effects of explosive injury which are independent of blood loss, include bradycardia, hypotension, and myocardial depression without a compensatory increase in peripheral vascular resistance (17). This response is likely driven by a vagal reflex (18) and overproduction of nitric oxide (19). These effects, which would not be accounted for by an anatomically based scoring system like the ISS, might result in prolonged ischemia to the kidneys with resultant increase in UB concentrations.…”

Section: Discussionmentioning

confidence: 99%

Urinary Biomarkers are Associated with Severity and Mechanism of Injury

Janak

Stewart

Sosnov

et al. 2017

Shock

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Combat-related blast trauma results in massive tissue injury and tends to involve multiple systems. Further, an acute measure of injury severity based on underlying biological mechanisms may be important for the triage and treatment of these types of patients. We hypothesized that urinary biomarkers (UBs) would reflect severity of injury and that they would be elevated for blast injuries compared to gunshot wounds (GSW) in a cohort of combat casualties. We also postulated that UBs would be higher in patients with burns compared to patients with non-burn trauma in a civilian cohort. Among 80 service members who sustained combat-related injuries, we performed generalized estimating equations to compare differences in log-transformed concentrations of the UBs by both (1) injury severity and (2) injury mechanism. Among 22 civilian patients, we performed Kruskal-Wallis tests to compare differences for the UBs stratified by burn and non-burn trauma. In the military cohort, with the exception of IL-18, all UBs were significantly (p<0.05) higher for patients with a severe combat-related injury (Injury Severity Score≥25). In addition, all crude UBs concentrations were significantly higher for blast vs. GSW patients (p<0.05). After adjusting for injury severity score and time of UB draw, KIM-1 (2.80 vs. 2.31; p=0.03) and LFABP (−1.11 vs. −1.92; p=0.02) were significantly higher for patients with a blast mechanism of injury. There were no significant differences in UBs between burn and non-burn civilian trauma patients. Future studies are needed to understand the physiologic response to trauma and the extent that UBs reflect these underlying processes.

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Pulmonary Blast Injury Increases Nitric Oxide Production, Disturbs Arginine Metabolism, and Alters the Plasma Free Amino Acid Pool in Rabbits during the Early Posttraumatic Period

Cited by 29 publications

References 25 publications

Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood

Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood

Perioperative dynamics and significance of amino acid profiles in patients with cancer

Urinary Biomarkers are Associated with Severity and Mechanism of Injury

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