Pulmonary and serum antibody responses elicited in zebu cattle experimentally infected with<i>Mycoplasma mycoides</i>subsp.<i>mycoides</i>SC by contact exposure

Niang, Mamadou; Diallo, Mahamadou; Cissé, Ousmane; Koné, Mamadou; Doucouré, M.; Roth, James A.; Balcer-Rodrigues, V.; Dedieu, Laurence

doi:10.1051/vetres:2006032

Cited by 24 publications

(23 citation statements)

References 18 publications

(20 reference statements)

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“…Putative protective immune mechanisms include the following: (i) production of IFN-γ, which potentiates phagocytic and bactericidal activity of macrophages and stimulates opsonization by bovine neutrophils through its IgG2 promoting effect [12], and (ii) CD4-dependent proliferation of B cells (this study). Interestingly, IgA levels in bronchial lavage fluids have recently been correlated with reduced severity of CBPP [18]. Additionally, we have described for the first time two phenotypes of MmmSC-specific memory CD4…”

Section: Secretion Of Ifn-γ Upon Stimulation Withmentioning

confidence: 76%

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Totté

Rodrigues

YaYa³

et al. 2007

Vet. Res.

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-A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4 + T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.contagious bovine pleuropneumonia / Mycoplasma mycoides subsp. mycoides SC / vaccine / memory / CD4

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Section: Secretion Of Ifn-γ Upon Stimulation Withmentioning

confidence: 76%

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Totté

Rodrigues

YaYa³

et al. 2007

Vet. Res.

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“…Nonetheless, the c-ELISA seems to be more sensitive than the CFT in detecting chronic and subacute cases during experiments and in endemic areas (Niang et al 2006;Sidibé et al 2012). It is important to note that like any serological test, c-ELISA tests have limitations since they do not differentiate postinfection antibodies from postvaccination antibodies.…”

Section: Discussionmentioning

confidence: 98%

Seroprevalence of contagious bovine pleuropneumonia (CBPP) in Mali

Séry

Sidibé

Cissé

et al. 2014

Trop Anim Health Prod

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A serological survey to determine the prevalence of contagious bovine pleuropneumonia (CBPP) in Mali was carried out by using the competitive enzyme linked-immunosorbent test (c-ELISA) on 8007 serum samples systematically collected from 199 cattle herds collected throughout the whole country. Results showed a national prevalence of 18.11 % at the individual level and 85.93 % at the herd level. Significant variations in the individual prevalence were observed between regions of the country and ranged from 4.63 % in Tombouctou to 54.88 % in Kidal. At the herd level, although there were variations between regions, a high prevalence was constantly observed ranging from 60 to 100 %, hence confirming the endemic nature of the disease across the country. The CBPP risk varied also between regions and was very low in Tombouctou (odds ratio (OR) = 0.4) but very high in Kidal (OR = 9.8). Similarly, the risk seemed higher in the animals of the over 3-year age group (OR = 1.6) compared to the other age groups. It was also observed that there was a slightly higher risk (OR = 1.3) in the females than in the males. This study confirms the presence of CBPP across the country and should help to elaborate strategies for the effective control of the disease.

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“…The SC route of inoculation was used on the grounds of expediency (Davies et al 1968) but was discouraged due to doubts as to whether the results gave an accurate assessment of immunity. In-contact transmission method was later used extensively (Huebschle et al 2006;Niang et al 2006;Hamsten et al 2010;Totté et al 2010) because it approximates more closely to a natural infection (Provost et al 1987). However, the method employs an extra group of animals whose only role is to serve as donors of infective material, making it more expensive.…”

Section: Introductionmentioning

confidence: 99%

Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

Nkando

Wesonga

Kuria

et al. 2010

Trop Anim Health Prod

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A study was carried out to assess the effectiveness of a bronchoscope in administering a pathogenic field strain of Mycoplasma mycoides subsp. mycoides (MmmSC) in cattle challenge experiments. Out of 16 animals inoculated using the bronchoscope, 10 (62.2%) showed clinical disease as evidenced by fever and 15 (93.8%) displayed typical lesions of CBPP from which MmmSC was isolated. Serum samples collected weekly were tested by Complement Fixation Test (CFT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Antibodies to MmmSC were detected in 10 out of the 16 animals by the CFT and 11 out of the 16 animals by c-ELISA. The onset of clinical disease was as early as 2 days post-inoculation, and most of the animals developed clinical disease 2 to 3 weeks post-infection. These results clearly demonstrate that nasotracheal inoculation of pathogenic strain of MmmSC with the aid of a bronchoscope can lead to early onset of clinical disease; similar to previous studies but with higher numbers of animals showing clinical disease. This is in contrast with previous studies where early clinical disease was observed in as little as 15% of inoculated animals. This nasotracheal inoculation method using a bronchoscope can, therefore, be adopted for use in experimental challenge infections of cattle. This method is found to be a better replacement to the contact transmission method whose drawback includes extra cost of donor animals and unpredictable rate and timing of transmission from intubated to challenge animals.

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Pulmonary and serum antibody responses elicited in zebu cattle experimentally infected withMycoplasma mycoidessubsp.mycoidesSC by contact exposure

Cited by 24 publications

References 18 publications

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Seroprevalence of contagious bovine pleuropneumonia (CBPP) in Mali

Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

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