Pulmonary alveolar proteinosis. Treatment by massive bronchopulmonary lavage

Ramirez, José

doi:10.1001/archinte.119.2.147

Cited by 66 publications

(30 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…151,152 Over the years this original procedure has been refined and modified to achieve good results with less procedural difficulty and fewer complications. 95,126,[153][154][155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172] The modifications include routine use of general anesthesia, 153,154 larger lavage volume, 154,155 lobar lavage, 162 positional clearance, 161 and chest percussion. 159,161 Additionally, successful completion of bilateral sequential 126 and simultaneous 163 wholelung lavage in the same session has been achieved.…”

Section: Whole-lung Lavagementioning

confidence: 99%

Pulmonary Alveolar Proteinosis

Khan

Agarwal

2011

Respir Care

View full text Add to dashboard Cite

Section: Whole-lung Lavagementioning

confidence: 99%

Pulmonary Alveolar Proteinosis

Khan

Agarwal

2011

Respir Care

View full text Add to dashboard Cite

“…PAP has been attribut ed to the failure of the pulmonary clearance of the surfactant, due to quantitative or qualitative deficiency of alveolar phagocyto sis [9,10]. The patient had a long-standing leukopenia which could account for a de creased phagocytic ability [3], In addition, during the year prior to his death, a pre-leukemic state developed, as evidenced by the abnormal lymphocyte clone [1], the bone marrow picture at autopsy, and the peri pheral blast forms.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary Alveolar Proteinosis Associated with Fanconi’s Anemia

Eldar¹,

Shoenfeld²,

Zaizov³

et al. 1979

Respiration

View full text Add to dashboard Cite

show abstract

“…Four patients with alveolar proteinosis, diagnosed by a typical clinical course, radiographic and physiologic findings, and lung biopsy, underwent therapeutic pulmonary lavage with 15-20 liters of isotonic saline under the direction of either Dr. J. Ramirez-R. (1 patient) or Dr. J. Kylstra (3 patients) (2,3). The lavage material was centrifuged at 5000 X g for 20 min to obtain a thick, yellow precipitate and a clear supernatant.…”

Section: Methodsmentioning

confidence: 99%

“…This material, which is insoluble in isotonic saline, can be removed in particulate form by pulmonary lavage (2,3). The extractable lipid from the lavage material contains large amounts of phosphatidyl choline and cholesterol (4).…”

mentioning

confidence: 99%

Isolation and Characterization of Two Glycoproteins from Patients with Alveolar Proteinosis

Passero

Tye

Kilburn

et al. 1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The saline-insoluble particulate material obtained by pulmonary lavage from four patients with alveolar proteinosis was analyzed for total lipid (52%), protein (44%), and carbohydrate (4%). Sodium dodecyl sulfate-gel electrophoresis revealed only three major peptides in these patients. Molecular weights (from the gels) were 69,000, 62,000, and 36,000, and the latter two peptides were periodic acid-Schiff stain-positive. Amino-acid and sugar analyses were performed on the peptides cut from the gels and on peptides purified by Sephadex G-200 chromatography. The 69,000-molecular-weight peptide contained nor carbohydrate. Antibody studies and aminoacid analysis indicated that it was albumin. The 62,000-molecular-weight peptide contained 1% hydroxyproline, 1% hydroxylysine, 10% glycine, and 9% carbohydrate.The 36,000-molecular-weight peptide contained 1.2% hydroxyproline, 1% hydroxylysine, 13% glycine, 1.4% sialic acid, 2.6% glucose, 2.4% galactose, 2% mannose, 0.8% fueose, 0.4% glycosamine, and 0.6% galactosamine. Proteins extracted from kidney glomeruli with similar amino-acid and carbohydrate composition have been observed previously. Alveolar proteinosis, first described by Rosen, Castleman, and Liebow in 1958 (1), is a chronic pulmonary disease of unknown pathogenesis in which the alveoli and terminal bronchioles of the lung are filled with a periodic acid-Schiff-positive amorphous material. This material, which is insoluble in isotonic saline, can be removed in particulate form by pulmonary lavage (2, 3). The extractable lipid from the lavage material contains large amounts of phosphatidyl choline and cholesterol (4). In the present study, the major peptides from the saline-insoluble lavage material have been isolated and characterized. METHODSFour patients with alveolar proteinosis, diagnosed by a typical clinical course, radiographic and physiologic findings, and lung biopsy, underwent therapeutic pulmonary lavage with 15-20 liters of isotonic saline under the direction of either Dr. J. Ramirez-R. (1 patient) or Dr. J. Kylstra (3 patients) (2, 3). The lavage material was centrifuged at 5000 X g for 20 min to obtain a thick, yellow precipitate and a clear supernatant. Both fractions were stored at -20°.Solubilization and Sodium Dodecyl Sulfate (SDS) Gel Electrophoresis of the Lavage Precipitate. The lavage precipitate was homogenized four times in cold isotonic saline and collected by centrifugation (15,000 X g for 15 min). Total protein was determined by the Lowry et al. method (5), and total carbohydrate was determined by the phenol-sulfuricacid method (6), and the thiobarbituric-acid assay for sialic acid (7). Total lipid was estimated gravimetrically after extraction with chloroform-methanol (2: 1 by volume).The lavage precipitate was dissolved in 0.2% SDS (Fisher)-0.1% 2-mercaptoethanol (Eastman)-0.04 M Tris-HCl (Schwarz-Mann) (pH 7.5). Solubilization of this material could be achieved only in the presence of thiol reagents. A black substance, representing less than 5% of the material, was precip...

show abstract

Pulmonary alveolar proteinosis. Treatment by massive bronchopulmonary lavage

Cited by 66 publications

References 13 publications

Pulmonary Alveolar Proteinosis

Pulmonary Alveolar Proteinosis

Pulmonary Alveolar Proteinosis Associated with Fanconi’s Anemia

Isolation and Characterization of Two Glycoproteins from Patients with Alveolar Proteinosis

Contact Info

Product

Resources

About