Pullulan in biomedical research and development - A review

Singh, Ram Sarup; Kaur, Navpreet; Hassan, Muhammad; Kennedy, John F.

doi:10.1016/j.ijbiomac.2020.10.227

Cited by 91 publications

(34 citation statements)

References 166 publications

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“…In order to investigate novel ATPS systems and w/w emulsions including new polysaccharides, pullulan (Pull) is a viable choice. 32,33 Pullulan consists of maltotriose units coupled via 1,6-α-bonds 34 and is well-known for its application in biomedicine, 35,36 e.g. as a carrier for gene delivery 36,37 and to form polysaccharide nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

pH sensitive water-in-water emulsions based on the pullulan and poly(N,N-dimethylacrylamide) aqueous two-phase system

Plucinski

Schmidt

2022

Polym. Chem.

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Section: Introductionmentioning

confidence: 99%

pH sensitive water-in-water emulsions based on the pullulan and poly(N,N-dimethylacrylamide) aqueous two-phase system

Plucinski

Schmidt

2022

Polym. Chem.

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“…This result could also explain why the exopolysaccharides produced by strain 06 showed remarkably higher viscosity at similar EPS concentration compared with other strains (Figures 1A, B). Because pullulan as a straight chain polymer, the property of viscosity was mainly affected by its Mw and concentration (Singh et al, 2021). Thus, Frontiers in Bioengineering and Biotechnology frontiersin.org the ability to produce high-Mw pullulan endowed A. pullulans BL06 with important value in scientific research and industrial applications.…”

Section: Production Properties Of the Yeast-like Fungus Aureobasidium...mentioning

confidence: 99%

“…Pullulan, composed of repeating maltotriose units connected by α-(1, 6) glycosidic bonds, is a linear extracellular polysaccharide (EPS) that is usually produced by the yeast-like fungus Aureobasidium pullulans ( A. pullulans ) ( Singh et al, 2021 ; Wei et al, 2021 ). It exhibits a wide range of applications in biomedicine, environmental engineering, and food engineering because it is a renewable, toxin-free, non-immunogenic and non-carcinogenic natural polymer ( Singh et al, 2019 ; Nishimura et al, 2020 ; An et al, 2021 ; Feng et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06

Chen

Zheng

Gao

et al. 2023

Front. Bioeng. Biotechnol.

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Pullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, the yield and molecular properties of pullulan produced by various strains still need to be promoted to fit the application needs. A novel yeast-like strain Aureobasidium pullulans BL06 producing high molecular weight (Mw) pullulan (3.3 × 106 Da) was isolated and identified in this study. The remarkable Mw of pullulan produced by A. pullulans BL06 was the highest level ever reported thus far. To further regulate the biosynthesis of pullulan in A. pullulans BL06, three gene knockout strains A. pullulans BL06 ΔPMAs, A. pullulans BL06 Δmel, and A. pullulans BL06 ΔPMAsΔmel, were constructed. The results showed that A. pullulans BL06 ΔPMAs could produce 140.2 g/L of moderate Mw (1.3 × 105 Da) pullulan after 120 h of fermentation. The highest yield level of pullulan to date could vastly reduce its production cost and expand its application scope and potential. The application experiments in food preservation showed that the moderate-Mw pullulan obtained in this work could reduce the weight loss of celery cabbages and mangos by 12.5% and 22%, respectively. Thus, the novel strains A. pullulans BL06 and A. pullulans BL06 ΔPMAs possessed unlimited development prospects in pullulan production at various Mw ranges and pullulan applications in multiple fields.

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“…Aureobasidium is a ubiquitous, saprophytic, dematiaceous fungus found in fresh water, oil-treated wood, and even in houses [ 1 – 3 ]. It produces various useful materials, such as pullulan [ 4 ], which is used as a biocontrol product against post-harvest diseases of fruits and vegetables [ 5 ], and cosmetic and biomedical applications, such as drug delivery, gene targeting, tissue engineering, and vaccination [ 6 ]. Therefore, research on its bioproducts is a hot topic in several fields, including biotechnology [ 7 ] and biomedical research [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Aureobasidium melanigenum catheter-related bloodstream infection: a case report

et al. 2022

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Background Aureobasidium melanigenum is a ubiquitous dematiaceous fungus that rarely causes invasive human infections. Here, we present a case of Aureobasidium melanigenum bloodstream infection in a 20-year-old man with long-term catheter use. Case presentation A 20-year-old man receiving home care with severe disabilities due to cerebral palsy and short bowel syndrome, resulting in long-term central venous catheter use, was referred to our hospital with a fever. After the detection of yeast-like cells in blood cultures on day 3, antifungal therapy was initiated. Two identification tests performed at a clinical microbiological laboratory showed different identification results: Aureobasidium pullulans from matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and Cryptococcus albidus from a VITEK2 system. Therefore, we changed the antifungal drug to liposomal amphotericin B. The fungus was identified as A. melanigenum by DNA sequence-based analysis. The patient recovered with antifungal therapy and long-term catheter removal. Conclusion It is difficult to correctly identify A. melanigenum by routine microbiological testing. Clinicians must pay attention to the process of identification of yeast-like cells and retain A. melanigenum in cases of refractory fungal infection.

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Pullulan in biomedical research and development - A review

Cited by 91 publications

References 166 publications

pH sensitive water-in-water emulsions based on the pullulan and poly(N,N-dimethylacrylamide) aqueous two-phase system

pH sensitive water-in-water emulsions based on the pullulan and poly(N,N-dimethylacrylamide) aqueous two-phase system

High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06

Aureobasidium melanigenum catheter-related bloodstream infection: a case report

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