Pullulan: a new cytoadhesive for cell-mediated cartilage repair

Abstract

IntroductionLocal delivery of mesenchymal stem cells (MSCs) to the acutely injured or osteoarthritic joint retards cartilage destruction. However, in the absence of assistive materials the efficiency of engraftment of MSCs to either intact or fibrillated cartilage is low and localization is further reduced by natural movement of the joint surfaces. It is hypothesised that enhanced engraftment of the delivered MSCs at the cartilage surface will increase their reparative effect and that the application of a bioadhesive to the degraded cartilage surface will provide improved cell retention. Pullulan is a structurally flexible, non-immunogenic exopolysaccharide with wet-stick adhesive properties and has previously been used for drug delivery via the wet surfaces of the buccal cavity. In this study, the adhesive character of pullulan was exploited to enhance MSC retention on the damaged cartilage surface.MethodsMSCs labeled with PKH26 were applied to pullulan-coated osteoarthritic cartilage explants to measure cell retention. Cytocompatability was assessed by measuring the effects of prolonged exposure to the bioadhesive on MSC viability and proliferation. The surface phenotype of the cells was assessed by flow cytometry and their multipotent nature by measuring osteogenic, adipogenic and chrondrogenic differentiation. Experiments were also carried out to determine expression of the C-type lectin Dectin-2 receptor.ResultsMSCs maintained a stable phenotype following exposure to pullulan in terms of metabolic activity, proliferation, differentiation and surface antigen expression. An increase in osteogenic activity and Dectin-2 receptor expression was seen in MSCs treated with pullulan. Markedly enhanced retention of MSCs was observed in explant culture of osteoarthritic cartilage.ConclusionsPullulan is a biocompatible and effective cytoadhesive material for tissue engraftment of MSCs. Prolonged exposure to pullulan has no negative impact on the phenotype, viability and differentiation potential of the cells. Pullulan dramatically improves the retention of MSCs at the fibrillated surface of osteoarthritic articular cartilage. Pullulan causes an upregulation in expression of the Dectin-2 C-type lectin transmembrane complex.

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

“…This strategy yielded enhanced wound healing of diabetic wounds which have a notoriously poor repair capability similar to OA lesions in articular cartilage. Prolonged exposure to pullulan had no negative impact on the phenotype, viability, and differentiation potential of the cells [428] with MSCs maintaining a stable phenotype in terms of metabolic activity, proliferation, and differentiation. Thus, ADSCs maintained their regenerative ability when delivered into wound sites using this hydrogel.…”

Influence of scaffold design on 3D printed cell constructs

“…34,35 Pullulan has been investigated in a recent study as a carrier platform for mesenchymal stem cells (MSCs) on an osteoarthritic explant. 36 Results illustrated the biocompatibility of pullulan as MSCs exhibited a stable phenotype following exposure to 0%, 2%, and 5% pullulan-containing medium and also an increase in osteogenic potential. Live/dead assays have also highlighted the nontoxic behavior of pullulan with the presence of elongated SMCs on pullulan hydrogel faces after 7 days.…”

“…It has been investigated as a bioadhesive and hydrogel for cell encapsulation and proliferation in previous studies . Pullulan has been investigated in a recent study as a carrier platform for mesenchymal stem cells (MSCs) on an osteoarthritic explant . Results illustrated the biocompatibility of pullulan as MSCs exhibited a stable phenotype following exposure to 0%, 2%, and 5% pullulan‐containing medium and also an increase in osteogenic potential.…”

Electrospinning Live Cells Using Gelatin and Pullulan

“…Trials with collagen and PEO were unsuccessful as cell viability was not acceptable. Highly hydrated polymers, such as PEO, suppress cellular and molecular adhesions by providing a physical steric barrier [35,36].…”