Pulling Nanotubes from Supported Bilayers

Armond, Jonathan W.; Macpherson, Julie V.; Turner, Matthew S.

doi:10.1021/la200639b

Cited by 9 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AFM force spectroscopy measurements rely both on approach and retraction curves. Usually, in the case of lipid bilayers, the approach curve is used for extracting nanomechanical information but, in some cases, valuables data can also be contained in retraction curve (Dufrêne et al, 1998;Armond et al, 2011). For example, the retraction curve can provide an indication of the adhesion force between the AFM tip and the different regions of the lipid bilayer (Dufrêne et al, 1998).…”

Section: Atomic Force Spectroscopy On Supported Lipid Bilayersmentioning

confidence: 99%

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Alessandrini

Facci

2012

Micron

View full text Add to dashboard Cite

The mechanical properties of biological systems are emerging as fundamental in determining their functional activity. For example, cells continuously probe their environment by applying forces and, at the same time, are exposed to forces produced by the same environment. Also in biological membranes, the activity of membrane related proteins are affected by the overall mechanical properties of the hosting environment. Traditionally, the mesoscopic mechanical properties of lipid bilayers have been studied by micropipette aspiration techniques. In recent years, the possibility of probing mechanical properties of lipid bilayers at the nanoscale has been promoted by the force spectroscopy potentiality of Atomic Force Microscopes (AFM). By acquiring force-curves on supported lipid bilayers (SLBs) it is possible to probe the mechanical properties on a scale relevant to the interaction between membrane proteins and lipid bilayers and to monitor changes of these properties as a result of a changing environment. Here, we review a series of force spectroscopy experiments performed on SLBs with an emphasis on the functional consequences the measured mechanical properties can have on membrane proteins. We also discuss the force spectroscopy experiments on SLBs in the context of theories developed for dynamic force spectroscopy experiments with the aim to extract the kinetic and energetic description of the process of membrane rupture.

show abstract

Section: Atomic Force Spectroscopy On Supported Lipid Bilayersmentioning

confidence: 99%

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Alessandrini

Facci

2012

Micron

View full text Add to dashboard Cite

show abstract

“…This tube pulling process occurs at constant force, the tube growing force (Ftube) (Armond et al, 2011;Berta Gumí-Audenis et al, 2018;Maeda et al, 2002;Ralf P Richter & Brisson, 2003), and it is observed as a force plateau in the retract part of the force-separation curve ( Figure 5), at several tens of pN from which Ftube and the tube growing distance (d) can be recorded. Pulling lipid tubes with an AFM tip out of SLBs is a simplified but analogous situation to some biological processes involving mechanical and conformational adjustments -bending, vesiculation, tubulation-like formation of membrane tethers, endocytic vesicles, etc.…”

Section: Atomic Force Microscopy (Afm)-based Methodologymentioning

confidence: 99%

“…However, the cytoskeleton adhesion and σ are difficult terms to separate, and therefore the concept of apparent membrane tension, σapp, has been proposed to include the adhesion energy parameter γ (Sheetz, 2001), σapp= σ + γ. When the lipid tube grows under thermodynamic equilibrium, at the limit of zero velocity (static thermodynamic analysis), the following mathematical expression relates the membrane parameters mentioned above with the Ftube (Armond et al, 2011;Canham, 1970;Daillant et al, 2005;E Evans & Yeung, 1994;Berta Gumí-Audenis et al, 2018;Hochmuth et al, 1996;Marcus & Hochmuth, 2002;Roux, 2013) The plus of this approach, AFM-based pulling lipid tubes from SLBs, is that it combines the advantage of the AFM local probing with nanoscale lateral and force resolution, with the simplicity of the SLB model preparation. Besides, the local nanomechanical properties of SLBs can be explored through the combination of the tubing force spectroscopy approach and the Fb analysis exposed before, within the same experimental procedure (Berta Gumí-Audenis et al, 2018).…”

Section: Atomic Force Microscopy (Afm)-based Methodologymentioning

confidence: 99%

Lipid bilayers: Phase behavior and nanomechanics

Redondo-Morata

Losada-Pérez

Giannotti

2020

Current Topics in Membranes

View full text Add to dashboard Cite

Lipid membranes are involved in many physiological processes like recognition, signaling, fusion or remodeling of the cell membrane or some of its internal compartments. Within the cell, they are the ultimate barrier, while maintaining the fluidity or flexibility required for a myriad of processes, including membrane protein assembly. The physical properties of in vitro model membranes as model cell membranes have been extensively studied with a variety of techniques, from classical thermodynamics to advanced modern microscopies. Here we review the nanomechanics of solid-supported lipid membranes with a focus in their phase behavior. Relevant information obtained by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM) as complementary techniques in the nano/mesoscale interface is presented. Membrane morphological and mechanical characterization will be discussed in the framework of its phase behavior, phase transitions and coexistence, in simple and complex models, and upon the presence of cholesterol.

show abstract

“…Typical marks for lipid bilayers adhering to the tip are bilayer penetration events ( Figure 3B ), a non-sharp transition when touching the surface ( Figure 3C ), or pulling of a lipid bilayer tether during retraction ( Figures 3B,C ). The latter is marked by a force plateau of 0.005–0.15 nN (Bo and Waugh, 1989 ; Armond et al, 2011 ).…”

Section: Materials and Equipmentmentioning

confidence: 99%

Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol

Vorselen

Piontek

Roos

et al. 2020

Front. Mol. Biosci.

View full text Add to dashboard Cite

Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (<200 nm) perform essential functions in cell biology and artificial vesicles (liposomes) are used as drug delivery vehicles. Atomic Force Microscopy (AFM) is a powerful technique to study the structural properties of these vesicles. AFM is a well-established technique for imaging at nanometer resolution and for mechanical measurements under physiological conditions. Here, we describe the procedure of AFM imaging and force spectroscopy on small vesicles. We discuss how to image vesicles with minimal structural disturbance, and how to analyze the data for accurate size and shape measurements. In addition, we describe the procedure for performing nanoindentations on vesicles and the subsequent data analysis including mechanical models used for data interpretation.

show abstract

Pulling Nanotubes from Supported Bilayers

Cited by 9 publications

References 34 publications

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Nanoscale mechanical properties of lipid bilayers and their relevance in biomembrane organization and function

Lipid bilayers: Phase behavior and nanomechanics

Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol

Contact Info

Product

Resources

About