Puerarin, isolated from Pueraria lobata (Willd.), protects against diabetic nephropathy by attenuating oxidative stress

Xu, Xiaogang; Zheng, Na; Chen, Zhaoni; Huang, Wansu; Liang, Tao; Kuang, Hai

doi:10.1016/j.gene.2016.06.032

Cited by 74 publications

(61 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with these findings, recent studies reported that SIRT1 expression is increased in the diabetic kidney from mice treated with puerarin 20 . However, it remained unclear how puerarin regulates SIRT1 in the kidney cells.…”

Section: Discussionsupporting

confidence: 70%

“…Anti-oxidative activity of puerarin has been demonstrated in cardiovascular 32 and neurological diseases 33 . Several studies also suggest that puerarin has anti-oxidative effects in kidney cells 19 , 20 , 22 , 23 . However, the mechanism by which puerarin exerts these effects remained unclear.…”

Section: Discussionmentioning

confidence: 99%

“…We and others have also shown that puerarin improve albuminuria in rodent models of streptozotocin (STZ)-induced diabetes 19 – 21 , although the extent of kidney injury was relatively mild in these models to clearly delineate the renoprotective effects of puerarin. Several studies have also shown both in vitro and in vivo that puerarin exhibited an anti-oxidative activity 19 , 20 , 22 , 23 , and that it attenuated apoptosis of proximal tubular cells through the restoration of mitochondrial function 24 , 25 . However the detailed mechanisms by which puerarin exerts renoprotection in DN had not been explored.…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Puerarin attenuates diabetic kidney injury through the suppression of NOX4 expression in podocytes

Cai

Lee

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Radix puerariae, a traditional Chinese herbal medication, has been used to treat patients with diabetic nephropathy (DN). Several studies demonstrated that puerarin, the active compound of radix puerariae, reduces diabetic injury in streptozotocin (STZ)-induced diabetic rodent models. However, as STZ injection alone results in mild kidney injury, the therapeutic benefit afforded by puerarin in DN remained inconclusive. Thus we sought to clarify the role of puerarin by employing an accelerated DN model, STZ-induced diabetes in the endothelial nitric oxide synthase-null (eNOS−/−) mice. Puerarin treatment of diabetic eNOS−/− mice significantly attenuated albuminuria and diabetic kidney injury, which were associated with reduced oxidative stress and reduced NAPDH oxidase 4 (NOX4) in glomeruli of diabetic eNOS−/− mice. Puerarin treatment of murine podocytes culture in high glucose conditions led to reduced superoxide production and NOX4 expression. We further determined that that puerarin treatment increased both mRNA and protein levels of SIRT1 in podocytes and that puerarin led to SIRT1-mediated deacetylation of NF-κB and suppression of NOX4 expression. Our findings confirm the renoprotective effects of puerarin in an experimental model of advanced DN and provide a molecular mechanism by which puerarin exerts the anti-oxidative effects in podocytes in the diabetic milieu.

show abstract

Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Puerarin attenuates diabetic kidney injury through the suppression of NOX4 expression in podocytes

Cai

Lee

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This improvement effect in kidney function may be attributed to antioxidant properties and phytoconstituents of beet root pomace. These results confirmed the results of Xu et al (2016) showed that flavonoids protected against diabetic nephropathy in STZ˗induced diabetic mice improving blood urea nitrogen, serum creatinine and urine protein levels as well as kidney tissue damage, with a reduction in mitochondrial damage.…”

Section: Resultssupporting

confidence: 88%

Potential Therapeutic Effects of Beet Root) Beta Vulgaris, L.) Pomace on Nephropathy Diabetic Rats

El-Sheikh

Nasef

Othman

2019

Alexandria Science Exchange Journal

View full text Add to dashboard Cite

Beet root possess widespread use in many traditional dishes, whereas its consumption has many highly health and therapeutic benefits to a human body attributed to its high content of phytochemical compounds and high antioxidant properties. The objectives of this study was to assess the potential therapeutic effects of beet root pomace powder (BRPP) on nephropathy induced by gentamicin in diabetic rats and study its application in cakes to utilization of it. Thirty adult male albino rats were randomly divided into two main groups and fed on standard diet. Group I: negative control (6 rats) and group II: nephropathy diabetic rats (24 rats) were given a single dose via intraperitoneal injection of 65 mg / kg body weight of streptozotocin (STZ). Then, Nephropathy was induced in diabetic rats via intraperitoneally injection daily of gentamicin (GM) 85 mg / kg body weight for 8 days. Nephropathy diabetic rats group were divided into four subgroups (6 rats each) as follow: First (positive control group), second, third and fourth group received standard diet + 2.5, 5 and 7.5 % of dried BRPP, respectively for 60 days. The results showed that BRPP contained (416.2 mg gallic acid/100 g) total phenolics, (31.3 mg catchin/100 g) flavonoids, (29.2 mg/100 g) β. carotene, (2.91 mg/100 g) ascorbic acid, (166.5 mg/100 g) betalain and had (42.87%) of antioxidant activity. Injection the rats with streptozotocin and gentamicin resulted in elevation in blood and urine glucose levels, lipid profile, kidney functions in the serum and MDA levels with a reduction in the activity of GSH.px, SOD and CAT in kidney tissues and serum insulin levels. Moreover, feeding nephropathy diabetic rats on a daily diet containing BRPP at 5 and 7.5% of standard diet for 60 days produced a marked reduction in the serum levels of blood and urine glucose, lipid profile, kidney function and MDA levels as well as elevation in the activity of GSH.px, SOD and CAT and serum insulin levels compared to the positive control group. Supplemented rats diet with 7.5% of BRPP was more effective in improving the previous parameters. Sensory evaluation of cakes prepared with 7.5% of BRPP showed the better properties in appearance, taste, texture, compressibility, colour and over all acceptability compared with the control and cakes prepared with 2.5 and 5% of BRPP. The study concluded that BRPP has hypoglycemic potent effects and improve renal damage induced by gentamicin in diabetic rats. So, it is advice to add BRPP to bakery products and consume daily for kidney patients.

show abstract

“…Therefore, the regulatory effect of puerarin on autophagy could be tissue or cell-specific. In DN, in addition to autophagy, several other mechanisms have been proposed for its renoprotective effects such as anti-oxidant and antiapoptotic effects (Yifei et al, 2014;Zhang et al, 2015;Song et al, 2016;Xu et al, 2016). Additional studies are necessary to clarify the contribution of each mechanism in mediating the renal protective effect of puerarin and how they interact each other.…”

Section: Discussionmentioning

confidence: 99%

Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes

Zhu

Zheng

et al. 2020

Front. Physiol.

View full text Add to dashboard Cite

Puerarin, an active compound of radix puerariae, is a major compound used in Chinese herbal medicines to treat patients with diabetic nephropathy (DN). In the previous studies, we showed that puerarin exerts renoprotective effects in Streptozocin (STZ)induced diabetic mice through activation of Sirt1 and anti-oxidative effects. Here, we further investigated the underlying mechanism mediating the renal protective effects of puerarin in DN. We studied the effects and mechanism of puerarin in STZ-induced diabetic mice and in cultured immortalized mouse podocytes treated with high glucose. We confirmed that puerarin ameliorated urinary albumin creatinine ratio and kidney injury in STZ-induced DN mice. We found that expression of heme oxygenase 1 (HMOX-1) and Sirt1 was suppressed in diabetic glomeruli but restored by puerarin treatment at both mRNA and protein levels. Additionally, we found that puerarin induced autophagy in the kidney of DN mice. In conditionally immortalized mouse podocytes, puerarin inhibited HG-induced apoptosis and restored the mRNA and protein levels of HMOX-1 and Sirt1. Interestingly, we showed that puerarin decreased liver kinase B1 (LKB1) acetylation, thereby promoting adenosine 5-monophosphate-activated protein kinase-dependent autophagy. Knockdown of HMOX-1 and Sirt1 expression or treatment with the autophagy inhibitor 3-methyladenine abolished the protective effects of puerarin in HG-treated podocytes. Taken together, these results suggest that puerarin protects podocytes from diabetes-induced injury through HMOX1 and Sirt1-mediated upregulation of autophagy, a novel mechanism explaining its renal protective effects in DN.

show abstract

Puerarin, isolated from Pueraria lobata (Willd.), protects against diabetic nephropathy by attenuating oxidative stress

Cited by 74 publications

References 20 publications

Puerarin attenuates diabetic kidney injury through the suppression of NOX4 expression in podocytes

Puerarin attenuates diabetic kidney injury through the suppression of NOX4 expression in podocytes

Potential Therapeutic Effects of Beet Root) Beta Vulgaris, L.) Pomace on Nephropathy Diabetic Rats

Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes

Contact Info

Product

Resources

About