Puerarin inhibits growth and induces apoptosis in SMMC-7721 hepatocellular carcinoma cells

Zhang, Weiguo; Liu, Xiaofang; Meng, Kewei; Hu, Sanyuan

doi:10.3892/mmr.2014.2512

Cited by 24 publications

(28 citation statements)

References 29 publications

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“…In addition, puerarin inhibited proliferation and induced apoptosis in U251 and U87 human glioblastoma cell lines, and cell cycle arrest and DNA damage may participate in the anticancer effects of puerarin (7). In SMMC-7721 hepatocellular carcinoma cells, puerarin suppressed cell viability and induced cell apoptosis (21). In human breast cancer cell lines HS578T, MDA-MB-231 and MCF-7, Puerariae radix isoflavones and their metabolites reduced cell growth and induced cell apoptosis, suggesting that puerariae may act as a chemotherapeutic agent against breast cancer (9).…”

Section: Discussionmentioning

confidence: 95%

Puerarin induces cell apoptosis in human chondrosarcoma cell line SW1353 via inhibition of the PI3K/Akt signaling pathway

Huang

Cao

et al. 2017

Oncol Lett

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Abstract. Chondrosarcoma is a malignant soft tissue sarcoma with poor prognosis. Puerarin has been demonstrated to possess anticancer properties; however, the effects of puerarin in human chondrosarcoma cells remain unknown. The present study aimed to investigate the anticancer effects of puerarin in SW1353 human chondrosarcoma cells. SW1353 cells were treated with increasing concentrations of puerarin for different durations. Cell viability was evaluated using MTT assays. Cell apoptosis rates were determined by flow cytometry. The activities of caspase-3 and caspase-9 were measured by enzymatic assay. The expression of RAC-alpha serine/threonine-protein kinase (Akt), phosphorylated-Akt, caspase-3 and apoptosis-associated proteins, including B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) were detected by western blotting. Puerarin significantly decreased cell viability and significantly induced apoptosis of SW1353 cells. In addition, puerarin significantly increased the enzymatic activities of caspase-3 and caspase-9. Puerarin treatment suppressed the expression of p-Akt and Bcl-2 but promoted the expression of Bax and cleaved caspase-3 in SW1353 cells. Notably, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 abrogated the decreased phosphorylation of Akt, suggesting that the PI3K/Akt signaling pathway is involved in mediating the anticancer effects of puerarin. The data from the present study indicated that puerarin exhibits anticancer effects in SW1353 cells and may be a potential therapeutic drug for patients with chondrosarcoma.

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Section: Discussionmentioning

confidence: 95%

Puerarin induces cell apoptosis in human chondrosarcoma cell line SW1353 via inhibition of the PI3K/Akt signaling pathway

Huang

Cao

et al. 2017

Oncol Lett

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“…There are several studies which have indicated that anticancer activity of puerarin is exerted by inhibition of proliferation of cancer cells. [11][12][13][14] We determined the effect of puerarin on proliferation of RB cells by cell colony formation assay. A significant reduction in the number of relative clone formation was observed after 24 h following puerarin treatment at 5 (P < 0.05), 10 (P < 0.01), and 15 (P < 0.01) µg/µL compared to control ( Figure 2B).…”

Section: Puerarin Inhibited the Proliferation Of Retinoblastoma Cellsmentioning

confidence: 99%

Retracted: Effects and mechanism of puerarin on the human retinoblastoma cells

Yang

Fan

2018

J of Cellular Biochemistry

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Puerarin is an isoflavonoid that is extracted from Kudzu root and is considered to have an anti-tumor effect. In the present study, the effects of puerarin on human retinoblastoma (RB) cells and the related pathways was determined. The retinoblastoma RB cell lines were used in this study. Cell viability and colony formation capacity were measured by MTT and colony formation assays. Cell cycle was determined by flow cytometry. Cell migration and invasion were examined by Transwell assay. The expression of cell cycle, EMT, and MAPK/ERK signal pathway-related proteins were detected by western blot following puerarin treatment. The results revealed that cell viability and proliferation of RB cells treated with puerarin were significantly lower in RB cells compared to the control group. Puerarin significantly decreased the proportion of cells during S phase which was accompanied with increase in cells at G0/1 and G2 phases. Moreover, puerarin suppressed cell migration, invasion and up-regulated E-Cadherin expression as well as down-regulated Vimentin and α-SMA expression. Furthermore, puerarin treatment suppressed the expression of p-MEK and p-ERK in RB cells. Our findings suggest that puerarin contributes to in the treatment of RB and other malignant tumors.

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“…The results showed that high concentrations of puerarin can inhibit the growth of breast cancer, and induce apoptosis of HT-29 colon cancer cells. Although the anti-tumor effect of puerarin in HCC has been verified (11), the molecular mechanism of puerarin in HCC is still unclear (12).…”

mentioning

confidence: 99%

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition

Zhou

Xue

Wang

et al. 2020

Braz J Med Biol Res

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Erratum for: "Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition" [Braz J Med Biol Res (2020) 53(4): e8882] Yuan Zhou 0 0 0 0 -0 0 0 0 -0 0 0 0 -0 0 0 0The authors would like to correct the Corresponding authors' names and emails and the Acknowledgments section of the article "Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition". The Editors highlight that the information presented in the original version was provided by the authors at submission and revision. In addition, the authors had the opportunity to correct the information during proofreading, before approving for publication.Correspondence: Haozhen Ren: orenhaozhen1984@163.com4 | Jinglin Wangocw20120817@163.com4 *These authors contributed equally to this study.Braz J Med Biol Res | AbstractHepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Inhibition of EMT has become a new therapeutic strategy for tumors. Recently, puerarin, a well-characterized component of traditional Chinese medicine, has been isolated from Pueraria radix and exerts positive effects on many diseases, particularly cancers. In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of puerarin on HCC cells. We further analyzed the relationship between puerarin and miR-21/PTEN/EMT markers in HCC cell lines. Our results showed that HCC cell proliferation, migration, invasion, tumor formation, and metastasis were reduced by puerarin in vitro and in vivo. Additionally, puerarin inhibited the EMT process of HCC by affecting the expression of Slug and Snail. Moreover, oncogenic miR-21 was inhibited by puerarin, coupled with an increase in the tumor suppressor gene PTEN. Increasing miR-21 expression or decreasing PTEN expression reversed the inhibition effects of puerarin in HCC. These data confirmed that puerarin affects HCC through the miR-21/PTEN/EMT regulatory axis. Overall, puerarin may represent a chemopreventive and/or chemotherapeutic agent for HCC treatment.

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Puerarin inhibits growth and induces apoptosis in SMMC-7721 hepatocellular carcinoma cells

Cited by 24 publications

References 29 publications

Puerarin induces cell apoptosis in human chondrosarcoma cell line SW1353 via inhibition of the PI3K/Akt signaling pathway

Puerarin induces cell apoptosis in human chondrosarcoma cell line SW1353 via inhibition of the PI3K/Akt signaling pathway

Retracted: Effects and mechanism of puerarin on the human retinoblastoma cells

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition

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