Puerarin induces platinum-resistant epithelial ovarian cancer cell apoptosis by targeting SIRT1

Duan, Jianxiu; Yin, Mingyuan; Shao, Yuan; Zheng, Jiao; Nie, Sheng

doi:10.1177/03000605211040762

Cited by 7 publications

(6 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SIRT1 is related to tumor cell proliferation, migration, invasion and angiogenesis [42,43]. A study found that after puerarin treatment of ovarian cancer cells, the expression of SIRT1 decreased and inhibited Wnt/β-catenin signaling pathway, thereby increasing the apoptosis of platinum-resistant ovarian cancer cells [44]. At present, many SIRT1 inhibitors have been reported, and whether puerarin can be used as a SIRT1 inhibitor related to the inhibition of NPC cells remains to be studied.…”

Section: Discussionmentioning

confidence: 99%

“…Puerarin can inhibit the proliferation and promote apoptosis of bladder cancer T24 cells, and can inactivate NF-κB signaling pathway [15]. Puerarin can also act on liver cancer [16], ovarian cancer [17], cervical cancer [18], esophageal cancer [19], and gastric cancer [20] through different mechanisms. However, there are few studies on puerarin in NPC.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect Of Puerarin on The Growth of Nasopharyngeal Carcinoma Cells and Its Impact on Angiogenesis

Li¹,

Zhao²,

Yang³

2022

JCRCT

View full text Add to dashboard Cite

Objective Puerarin is a form of isoflavones obtained from Pueraria lobata. It stimulates hepatic metabolic function and lowers serum ALT, AST, and total-bilirubin level. The purpose of this study was to examine the effect of puerarin on nasopharyngeal carcinoma (NPC) CNE1 cells and preliminarily explore its possible mechanism. Materials and Methods CCK8 method was used to detect the proliferation activity of puerarin on NPC CNE1 cells and IC50 was calculated. CNE1 cells were treated with 0 μmol/L puerarin (containing equal volume of DMSO solution) as control group and 1000 μmol/L puerarin (IC50 concentration) as experimental group. Colony formation assay, Scratch-wound test and Transwell invasion assay were used to detect the clone formation ability, migration and invasion ability of puerarin on CNE1 cells. Then, RNA Sequencing was used to detect the changes of differentially expressed genes (DEGs) and signaling pathways after puerarin was applied to CNE1 cells. Results The inhibitory effect of puerarin on the proliferation activity of CNE1 cells was enhanced with the increase of concentration, and IC50 was calculated as 1000 μmol/L. Compared with the control group, the treatment of CNE1 cells with 1000 μmol/L puerarin could inhibit the clone formation, migration and invasion of CNE1 cells (P<0.05). A total of 379 DEGs were found by RNA sequencing, including 295 down-regulated genes and 84 up-regulated genes (padj<0.05). The significant differences in biological functions of differentially expressed genes were mainly distributed in “negative regulation of growth”, “angiogenesis”, “regulation of peptidase activity”, “positive regulation of vasculature development”, “digestion”, “positive regulation of angiogenesis”, “negative regulation of peptidase activity”, “extracellular matrix” and “Golgi lumen” (padj<0.05). Conclusion Puerarin could inhibit the proliferation, migration and invasion of NPC CNE1 cells, and its mechanism might be related to the inhibition of angiogenesis and cell growth.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect Of Puerarin on The Growth of Nasopharyngeal Carcinoma Cells and Its Impact on Angiogenesis

Li¹,

Zhao²,

Yang³

2022

JCRCT

View full text Add to dashboard Cite

show abstract

“…Later, Duan et al found that gerberoside effectively inhibited ovarian cancer cell growth in vitro and in vivo by increasing apoptosis. More importantly, it was found that gerberoside decreased SIRT1 expression, thereby attenuating the nuclear accumulation of β-catenin and subsequently inhibiting Wnt/β-catenin signaling, thus sensitizing cisplatin-resistant ovarian cancer cells to chemotherapy [ 64 ]. Miyamoto et al found higher expression of SIRT1 in endometrioid, mucinous, and clear cell carcinomas compared to normal ovarian tissues and follicles, suggesting that high expression of SIRT1 is associated with cisplatin resistance in ovarian cancer cells and is one of the main reasons for poor prognosis in ovarian cancer patients [ 65 ].…”

Section: Effect Of Sirt1 On Premature Ovarian Failure and Ovarian Cancermentioning

confidence: 99%

Regulation of SIRT1 in Ovarian Function: PCOS Treatment

et al. 2023

CIMB

View full text Add to dashboard Cite

The sirtuin family, a group of NAD+-dependent class 3 histone deacetylases (HDACs), was extensively studied initially as a group of longevity genes that are activated in caloric restriction and act in concert with nicotinamide adenine dinucleotides to extend the lifespan. Subsequent studies have found that sirtuins are involved in various physiological processes, including cell proliferation, apoptosis, cell cycle progression, and insulin signaling, and they have been extensively studied as cancer genes. In recent years, it has been found that caloric restriction increases ovarian reserves, suggesting that sirtuins may play a regulatory role in reproductive capacity, and interest in the sirtuin family has continued to increase. The purpose of this paper is to summarize the existing studies and analyze the role and mechanism of SIRT1, a member of the sirtuin family, in regulating ovarian function. Research and review on the positive regulation of SIRT1 in ovarian function and its therapeutic effect on PCOS syndrome.

show abstract

“…Puerarin, as a natural flavonoid compound isolated from the root of Pueraria lobata, has been shown to have many bioactive functions (anti-inflammatory, antioxidant and antiviral [ 10 ]). Especially, previous studies showed that puerarin has a significant effect on the treatment of inflammatory diseases, such as cancer [ 11 ], diabetes [ 12 ], Alzheimer’s disease [ 13 ] and cardiovascular disease [ 14 ], which evoked the interest of researchers. At present, there are many studies on the anti-inflammatory activity of puerarin.…”

Section: Introductionmentioning

confidence: 99%

Puerarin Induces Molecular Details of Ferroptosis-Associated Anti-Inflammatory on RAW264.7 Macrophages

et al. 2022

View full text Add to dashboard Cite

Puerarin is a natural flavonoid with significant anti-inflammatory effects. Recent studies have suggested that ferroptosis may involve puerarin countering inflammation. However, the mechanism of ferroptosis mediated by the anti-inflammatory process of puerarin has not been widely explored. Herein, puerarin at a concentration of 40 μM showed an anti-inflammatory effect on lipopolysaccharide (LPS)-induced macrophages RAW264.7. The analysis of network pharmacology indicated that 51 common targets were enriched in 136 pathways, and most of the pathways were associated with ferroptosis. Subsequently, the analysis of metabolomics obtained 61 differential metabolites that were enriched in 30 metabolic pathways. Furthermore, integrated network pharmacology and metabolomics revealed that puerarin exerted an excellent effect on anti-inflammatory in RAW264.7 via regulating ferroptosis-related arachidonic acid metabolism, tryptophan metabolism, and glutathione metabolism pathways, and metabolites such as 20-hydroxyeicosatetraenoic acid (20-HETE), serotonin, kynurenine, oxidized glutathione (GSSG), gamma-glutamylcysteine and cysteinylglycine were involved. In addition, the possible active binding sites of the potential targeted proteins such as acyl-CoA synthetase long-chain family member 4 (ACSL4), prostaglandin-endoperoxide synthase 2 (PTGS2), arachidonate 15-lipoxygenase (ALOX15) and glutathione peroxidase 4 (GPX4) with puerarin were further revealed by molecular docking. Thus, we suggested that ferroptosis mediated the anti-inflammatory effects of puerarin in macrophages RAW264.7 induced by LPS.

show abstract

Puerarin induces platinum-resistant epithelial ovarian cancer cell apoptosis by targeting SIRT1

Cited by 7 publications

References 38 publications

Effect Of Puerarin on The Growth of Nasopharyngeal Carcinoma Cells and Its Impact on Angiogenesis

Effect Of Puerarin on The Growth of Nasopharyngeal Carcinoma Cells and Its Impact on Angiogenesis

Regulation of SIRT1 in Ovarian Function: PCOS Treatment

Puerarin Induces Molecular Details of Ferroptosis-Associated Anti-Inflammatory on RAW264.7 Macrophages

Contact Info

Product

Resources

About