Puerarin induces mouse mesenteric vasodilation and ameliorates hypertension involving endothelial TRPV4 channels

Zhou, Tingting; Wang, Zhiwei; Guo, Mengting; Zhang, Ka; Geng, Li; Mao, Aiqin; Yang, Yujiao; Yu, Fan

doi:10.1039/d0fo02356f

Cited by 27 publications

(5 citation statements)

References 57 publications

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“…Acute 40 min puerarin treatment was found to reduce EDCs in a concentration-dependent manner and failed to inhibit the production of PGs in intact endothelial arteries, suggesting that puerarin is able to inhibit EDCs induced via Ach independently of the inhibition of TP receptor-or COX2-derived PGs. Zhou et al [94] pretreated first-order mouse mesenteric resistance arteries with intact or incomplete endothelium using 1 µM phenylephrine (Phe) to constrict them and then exposed them to cumulative doses of puerarin (0.01, 0.1, 1, 10, 20, 50, 100, and 200 µM), and they found that mesenteric arteries with intact endothelium in mice treated with puerarin relaxed and that the degree of relaxation was correlated with the dose of puerarin. Subsequently, to investigate the role of transient receptor potential vanilloid 4 (TRPV4) channels in the modulation of vasorelaxation by puerarin, mesenteric arteries of TRPV4 −/− mice were selected.…”

Section: Regulates Vasodilationmentioning

confidence: 99%

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Gao,

Wang,

Huang

et al. 2024

Separations

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Kudzu root (Puerariae lobatae Radix) is the tuberous root of Pueraria lobata, family Leguminosae. Kudzu root contains a variety of beneficial active ingredients such as puerarin, daidzin, daidzein, genistenin, 3′-hydroxy puerarin, β-sitosterol, stigmasterol, arachidic acid, and so on. Modern medical research shows that active ingredients in kudzu root are widely used clinically as raw materials for the treatment of hyperglycemia, non-alcoholic fatty liver disease, myocardial infarction, alcohol addiction, oxidative stress, inflammatory response, and retinal blockage due to their various pharmacological effects such as improving cardiovascular circulation, lowering blood lipids, lowering blood pressure, lowering blood sugar, being antipyretic, being estrogen-like, and relieving alcohol. China has rich resources of kudzu root, and active ingredients are usually extracted before it is made into a preparation, so whether the extraction and separation process is reasonable will directly affect the ease of preparation and the efficacy of the treatment. This paper reviews the process methods for the extraction and separation of active ingredients in kudzu root and its common pharmacological activities. The aim is to provide some references for readers to compare the advantages and disadvantages of various extraction and separation methods as well as understand the active ingredients and pharmacological activities of kudzu root.

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Section: Regulates Vasodilationmentioning

confidence: 99%

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Gao,

Wang,

Huang

et al. 2024

Separations

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“…TSN binds to VDAC1. Molecular docking is widely used to assay potential bioactivity of compounds (36,37). computational modeling was performed to investigate the direct interaction between TSN and VdAc1.…”

Section: Tsn Attenuates Erastin-induced Ferroptosis and Inhibits Apop...mentioning

confidence: 99%

Tanshinone IIA confers protection against myocardial ischemia/reperfusion injury by inhibiting ferroptosis and apoptosis via VDAC1

Hu,

Zou,

et al. 2023

Int J Mol Med

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Tanshinone IIA (TSN) extracted from danshen ( Salvia miltiorrhiza ) could protect cardiomyocytes against myocardial ischemia/reperfusion injury (IRI), however the underlying molecular mechanisms of action remain unclear. The aim of the present study was to identify the protective effects of TSN and its mechanisms of action through in vitro studies. An anoxia/reoxygenation (A/R) injury model was established using H9c2 cells to simulate myocardial IRI in vitro . Before A/R, H9c2 cardiomyoblasts were pretreated with 8 μ M TSN or 10 μ M ferrostatin-1 (Fer-1) or erastin. The cell counting kit 8 (CCK-8) and lactate dehydrogenase (LDH) assay kit were used to detect the cell viability and cytotoxicity. The levels of total iron, glutathione (GSH), glutathione disulfide (GSSG), malondialdehyde (MDA), ferrous iron, caspase-3 activity, and reactive oxygen species (ROS) were assessed using commercial kit. The levels of mitochondrial membrane potential (MMP), lipid ROS, cell apoptosis, and mitochondrial permeability transition pore (mPTP) opening were detected by flow cytometry. Transmission electron microscopy (TEM) was used to observed the mitochondrial damage. Protein levels were detected by western blot analysis. The interaction between TSN and voltage-dependent anion channel 1 (VDAC1) was evaluated by molecular docking simulation. The results showed that pretreatment with TSN and Fer-1 significantly decreased cell viability, glutathione peroxidase 4 (GPX4) protein and GSH expression and GSH/GSSG ratio and inhibited upregulation of LDH activity, prostaglandin endoperoxide synthase 2 and VDAC1 protein expression, ROS levels, mitochondrial injury and GSSG induced by A/R. TSN also effectively inhibited the damaging effects of erastin treatment. Additionally, TSN increased MMP and Bcl-2/Bax ratio, while decreasing levels of apoptotic cells, activating Caspase-3 and closing the mPTP. These effects were blocked by VDAC1 overexpression and the results of molecular docking simulation studies revealed a direct interaction between TSN and VDAC1. In conclusion, TSN pretreatment effectively attenuated H9c2 cardiomyocyte damage in an A/R injury model and VDAC1-mediated ferroptosis and apoptosis served a vital role in the protective effects of TSN.

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“…Transient receptor potential vanillin 4 (TRPV4) is a calcium permeability channel with diverse activation modes, which plays a crucial role in vascular endothelial function and vasodilation. As a TRPV4 agonist, puerarin induced endothelium-dependent vasodilation of mesenteric arteries in mice and reduced blood pressure in hypertensive mice induced by high salt, highlighting the beneficial role of puerarin in the treatment of endothelial-dysfunction-related cardiovascular diseases [ 96 ]. Puerarin also enhances vasodilation and insulin-stimulated Akt/eNOS pathways by inhibiting the NF-κB inflammatory pathway and decreasing plasma TNF-α levels [ 97 ].…”

Section: The Role Of Puerarin In Inflammatory Diseasesmentioning

confidence: 99%

Pharmacological Activity, Pharmacokinetics, and Clinical Research Progress of Puerarin

Wang

et al. 2022

Antioxidants

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As a kind of medicine and food homologous plant, kudzu root (Pueraria lobata (Willd.) Ohwi) is called an “official medicine” in Chinese folk medicine. Puerarin is the main active component extracted from kudzu root, and its structural formula is 8-β-D-grapes pyranose-4, 7-dihydroxy isoflavone, with a white needle crystal; it is slightly soluble in water, and its aqueous solution is colorless or light yellow. Puerarin is a natural antioxidant with high health value and has a series of biological activities such as antioxidation, anti-inflammation, anti-tumor effects, immunity improvement, and cardio-cerebrovascular and nerve cell protection. In particular, for the past few years, it has also been extensively used in clinical study. This review focuses on the antioxidant activity of puerarin, the therapy of diverse types of inflammatory diseases, various new drug delivery systems of puerarin, the “structure-activity relationship” of puerarin and its derivatives, and pharmacokinetic and clinical studies, which can provide a new perspective for the puerarin-related drug research and development, clinical application, and further development and utilization.

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Puerarin induces mouse mesenteric vasodilation and ameliorates hypertension involving endothelial TRPV4 channels

Abstract: Puerarin derived from Pueraria induces mesenteric endothelium-dependent vasodilation involving the TRPV4-IKCa/SKCa pathway, exhibiting antihypertensive activity against high-salt-induced hypertension.

Cited by 27 publications

References 57 publications

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Tanshinone IIA confers protection against myocardial ischemia/reperfusion injury by inhibiting ferroptosis and apoptosis via VDAC1

Pharmacological Activity, Pharmacokinetics, and Clinical Research Progress of Puerarin

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