Puerariae Lobatae radix flavonoids and puerarin alleviate alcoholic liver injury in zebrafish by regulating alcohol and lipid metabolism

Liu, Yu-Shi; Yuan, Minghao; Zhang, Cunyan; Li, Hongmei; Liu, Juanru; Wei, Ailing; Ye, Qiang; Zeng, Bin; Li, Mei-feng; Guo, Yiping; Guo, Li

doi:10.1016/j.biopha.2020.111121

Cited by 51 publications

(35 citation statements)

References 43 publications

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“…However, to assess fatty acid mobilization and its transportation, the lipophilic fluorophore BODIPY ® can be used [ 36 ]. Zebrafish are useful model organisms for in vivo testing, dedicated to preclinical tests; indeed, this approach allows the screening of large libraries of natural compounds including flavonoids for the treatment of obesity [ 37 , 38 ]. Drug screening is directly conducted on zebrafish larvae since they have the signal transduction pathways regulating lipid metabolism [ 39 ].…”

Section: Obesity: Model Of Choicementioning

confidence: 99%

“…The above-mentioned factor shortage leads to the downregulation of late adipogenic factors, negatively regulating the m-TOR signaling pathway, involved in lipid accumulation during adipogenesis and decreased p-p38 MAPK and pERK levels in adipocytes [ 95 , 96 ]. Puerariae Lobatae radix flavonoids and puerarin successfully limited lipid accumulation in the abdomens of zebrafish larvae in a dose dependent manner [ 38 ].…”

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

“…Thus, quercetin inhibited TG accumulation by >40%, while curcumin, an antiadipogenic phytochemical, inhibited TG accumulation by ~25% [ 91 ]. Baicalein, kaempferol, and eriocitrin, flavonoids that have lately been reported, significantly suppressed the increase in TG in DIO-zebrafish [ 38 , 93 , 95 ].…”

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

“…Many flavonoids, previously studied for anti-obesity effects, have the ability to reduce cholesterol levels. Puerariae Lobatae radix flavonoids and puerarin significantly downregulated the elevated mRNA levels of the 3-hydroxy-3-methylglutaryl coenzyme A reductase b (HMGCRB), the key enzyme involved in lipid metabolism and cholesterol biosynthesis, in zebrafish larvae [ 38 ]. On the other hand, baicalein decreased the expression levels of SREBP1 and fatty acid synthase (fasn) and regulated the synthesis and desaturation of fatty acids, proteins and a master gene regulating cholesterol synthesis.…”

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

“…In addition, quercetin has the ability to stimulate the amount of IL-10, a known inhibitory factor of cytokine synthesis and anti-inflammatory cytokine [ 91 ]. In studies conducted with Puerariae Lobatae radix flavonoids, puerarin, and Citrus sinensis flavonoids, they were observed to possess important anti-inflammatory activity, assured by the downregulation of inflammatory cytokine-related genes, such as IL-1β (interleukin-1β), IL-6 and TNFα (tumor necrosis factor-α) in a zebrafish model [ 38 , 100 ]. The flavonoid-rich extract of Withania somnifera leaf, essentially with kaempferol and agigenein, revealed the significant inhibition of TNFα in adult zebrafish [ 101 ].…”

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

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Zebrafish and Flavonoids: Adjuvants against Obesity

et al. 2021

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Obesity is a pathological condition, defined as an excessive accumulation of fat, primarily caused by an energy imbalance. The storage of excess energy in the form of triglycerides within the adipocyte leads to lipotoxicity and promotes the phenotypic switch in the M1/M2 macrophage. These changes induce the development of a chronic state of low-grade inflammation, subsequently generating obesity-related complications, commonly known as metabolic syndromes. Over the past decade, obesity has been studied in many animal models. However, due to its competitive aspects and unique characteristics, the use of zebrafish has begun to gain traction in experimental obesity research. To counteract obesity and its related comorbidities, several natural substances have been studied. One of those natural substances reported to have substantial biological effects on obesity are flavonoids. This review summarizes the results of studies that examined the effects of flavonoids on obesity and related diseases and the emergence of zebrafish as a model of diet-induced obesity.

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Section: Obesity: Model Of Choicementioning

confidence: 99%

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

Section: Flavonoids Obesity and Metabolic Syndromementioning

confidence: 99%

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Zebrafish and Flavonoids: Adjuvants against Obesity

et al. 2021

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Clavukoelloids A—M, Thirteen Rare N‐Containing Nardosinane Type Sesquiterpenoids from the Soft Coral Clavularia koellikeri

Han

Wang

et al. 2023

Chin. J. Chem.

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Comprehensive SummaryThirteen new nitrogen‐containing nardosinane‐type sesquiterpenoids, clavukoelloids A—M (1—13) with unique acrylamide fragments vs. the reported general nardosinane‐type sesquiterpenoids containing lactone, were isolated from the soft coral Clavularia koellikeri collected from the South China Sea. The structures and absolute configurations of new compounds were unambiguously determined by analysis of spectroscopic data, CP3, DP4+, ECD spectra, and X‐ray diffraction. Compound 6 showed anti‐inflammatory activities and hepatoprotective activities in transgenic fluorescent zebrafish.

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Chemical profiling and quality evaluation of Liuweizhiji Gegen‐Sangshen oral liquid by UPLC‐Q‐TOF‐MS and HPLC‐diode array detector fingerprinting

Wei,

Jiang,

et al. 2024

Phytochemical Analysis

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IntroductionLiuweizhiji Gegen‐Sangshen (LGS) oral liquid is a Chinese patent medicine that is widely used for the prevention and treatment of alcoholic liver disease in clinical practice. However, the chemical complexity of LGS has not yet been investigated.ObjectiveThe aim of this study was to rapidly identify chemical constituents of LGS and establish a quality control method based on fingerprint and quantitative analysis.MethodologyA comprehensive strategy was used by combining qualitative analysis by ultra‐performance liquid chromatography tandem quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS) and fingerprint analysis by high‐performance liquid chromatography with diode array detection (HPLC‐DAD).ResultsA total of 162 chemical components in LGS, including 91 flavonoids, 31 organic acids, and 20 phenolic compounds, were identified or preliminarily characterized in both positive and negative ion modes based on the UPLC‐Q‐TOF‐MS results. Of these, 37 were confirmed with the reference standards. In fingerprint analysis, 23 peaks were chosen as common peaks and used to evaluate the similarity of different batches of LGS. Subsequently, a rapid quantification method was optimized and validated for the simultaneous determination of multiple chemical markers in LGS. The validated quantitative method was successfully used to analyze different batches of LGS samples.ConclusionThe proposed comprehensive strategy combining HPLC‐DAD fingerprinting and multi‐component quantification demonstrated satisfactory results with high efficiency, accuracy, and reliability. This can be used as a reference for the overall quality consistency evaluation of Chinese patent medicines.

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Puerariae Lobatae radix flavonoids and puerarin alleviate alcoholic liver injury in zebrafish by regulating alcohol and lipid metabolism

Cited by 51 publications

References 43 publications

Zebrafish and Flavonoids: Adjuvants against Obesity

Zebrafish and Flavonoids: Adjuvants against Obesity

Clavukoelloids A—M, Thirteen Rare N‐Containing Nardosinane Type Sesquiterpenoids from the Soft Coral Clavularia koellikeri

Chemical profiling and quality evaluation of Liuweizhiji Gegen‐Sangshen oral liquid by UPLC‐Q‐TOF‐MS and HPLC‐diode array detector fingerprinting

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