Publisher Correction: RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3′UTR required for intermediate vancomycin resistance

Mediati, Daniel G.; Wong, Julia L.; Gao, Wei; McKellar, Stuart; Pang, Chi Nam Ignatius; Wu, Sylvania; Wu, Winton; Sy, Brandon; Monk, Ian R.; Biazik, Joanna; Wilkins, Marc R.; Howden, Benjamin P; Stinear, Timothy P.; Granneman, Sander; Tree, Jai J.

doi:10.1038/s41467-022-33167-2

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“…By examining RNA-RNA interactions, Mediati and collaborators identified a regulatory region within the 3′UTR of vigR mRNA. This vigR 3′UTR enhanced isaA expression, which is a cell wall lytic transglycosylase ( 61 ). Using CRISPRi, vigR mRNA expression was reduced, resulting in 1000-fold susceptibility to sub-inhibitory vancomycin concentrations.…”

Section: Gram-positive Bacteriamentioning

confidence: 99%

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Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Gager,

Flores-Mireles

2024

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The spread of multi-drug-resistant (MDR) pathogens has rapidly outpaced the development of effective treatments. Diverse resistance mechanisms further limit the effectiveness of our best treatments, including multi-drug regimens and last line-of-defense antimicrobials. Biofilm formation is a powerful component of microbial pathogenesis, providing a scaffold for efficient colonization and shielding against anti-microbials, which further complicates drug resistance studies. Early genetic knockout tools didn’t allow the study of essential genes, but clustered regularly interspaced palindromic repeat inference (CRISPRi) technologies have overcome this challenge via genetic silencing. These tools rapidly evolved to meet new demands and exploit native CRISPR systems. Modern tools range from the creation of massive CRISPRi libraries to tunable modulation of gene expression with CRISPR activation (CRISPRa). This review discusses the rapid expansion of CRISPRi/a-based technologies, their use in investigating MDR and biofilm formation, and how this drives further development of a potent tool to comprehensively examine multi-drug resistance.

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Section: Gram-positive Bacteriamentioning

confidence: 99%

“…Knockdown of isaA revealed reduction in cell wall thickness, which partially re-sensitized S. aureus to vancomycin. This effect was less successful CRISPRi inhibition of vigR 3′UTR, which may encourage further CRISPRi use in non-coding genetic regions ( 61 ).…”

Section: Gram-positive Bacteriamentioning

confidence: 99%

Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Gager,

Flores-Mireles

2024

mSphere

View full text Add to dashboard Cite

show abstract

Publisher Correction: RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3′UTR required for intermediate vancomycin resistance

Cited by 1 publication

References 0 publications

Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

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