Publisher Correction: Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts

Ларина, И. М.; Percy, Andrew J.; Yang, Juncong; Borchers, Christoph H.; Nosovsky, A. M.; Grigoriev, A. I.; Nikolaev, Evgeny N.

doi:10.1038/s41598-019-43121-w

Cited by 2 publications

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“…A recent study showed an increase in osteocyte senescence after irradiation [ 17 ]. Although PPIA has been identified as a very stable and GAPDH as fairly stable reference genes during in vitro senescence in mesenchymal stem cells [ 18 ], it is noteworthy that GAPDH expression decreases during in vivo aging in fibroblasts [ 19 ]. As in vivo molecular mechanisms of irradiation-induced osteocyte senescence have not been fully elucidated yet, we strongly suggest interpreting possible differences between group C and C irrad carefully.…”

Section: Discussionmentioning

confidence: 99%

Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats

Rottensteiner-Brandl

Bertram

Lingens

et al. 2021

Cells

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Healing of large bone defects remains a challenge in reconstructive surgery, especially with impaired healing potential due to severe trauma, infection or irradiation. In vivo studies are often performed in healthy animals, which might not accurately reflect the situation in clinical cases. In the present study, we successfully combined a critical-sized femoral defect model with an ionizing radiation protocol in rats. To support bone healing, tissue-engineered constructs were transferred into the defect after ectopic preossification and prevascularization. The combination of SiHA, MSCs and BMP-2 resulted in the significant ectopic formation of bone tissue, which can easily be transferred by means of our custom-made titanium chamber. Implanted osteogenic MSCs survived in vivo for a total of 18 weeks. The use of SiHA alone did not lead to bone formation after ectopic implantation. Analysis of gene expression showed early osteoblast differentiation and a hypoxic and inflammatory environment in implanted constructs. Irradiation led to impaired bone healing, decreased vascularization and lower short-term survival of implanted cells. We conclude that our model is highly valuable for the investigation of bone healing and tissue engineering in pre-damaged tissue and that healing of bone defects can be substantially supported by combining SiHA, MSCs and BMP-2.

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Section: Discussionmentioning

confidence: 99%

Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats

Rottensteiner-Brandl

Bertram

Lingens

et al. 2021

Cells

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“…In most cases, these proteins do not return to pre-flight baselines by day 7 after spaceflight. They are involved in the blood clotting system, ECM remodeling, and immune processes [ 104 ].…”

Section: Proteomic Profile Of Human Matrisome-associated Proteins Under Real and Simulated Microgravitymentioning

confidence: 99%

“…Latridis et al suggest that these reactions may be triggered by extracellular signaling pathways of mechanotransduction [ 105 ]. Part of the group of functionally ECM-associated proteins detected in the samples at day 1 after landing in a reduced concentration, were found to return to baseline after 7 days, but another part of proteins retained significantly reduced concentrations [ 103 , 104 ]. The ANDCell program made it possible to identify biological processes involving ECM proteins that are modified by spaceflight factors ( Table 2 ).…”

Section: Proteomic Profile Of Human Matrisome-associated Proteins Under Real and Simulated Microgravitymentioning

confidence: 99%

Microgravity Effects on the Matrisome

Буравкова

Ларина

Андреева

et al. 2021

Cells

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Gravity is fundamental factor determining all processes of development and vital activity on Earth. During evolution, a complex mechanism of response to gravity alterations was formed in multicellular organisms. It includes the “gravisensors” in extracellular and intracellular spaces. Inside the cells, the cytoskeleton molecules are the principal gravity-sensitive structures, and outside the cells these are extracellular matrix (ECM) components. The cooperation between the intracellular and extracellular compartments is implemented through specialized protein structures, integrins. The gravity-sensitive complex is a kind of molecular hub that coordinates the functions of various tissues and organs in the gravitational environment. The functioning of this system is of particular importance under extremal conditions, such as spaceflight microgravity. This review covers the current understanding of ECM and associated molecules as the matrisome, the features of the above components in connective tissues, and the role of the latter in the cell and tissue responses to the gravity alterations. Special attention is paid to contemporary methodological approaches to the matrisome composition analysis under real space flights and ground-based simulation of its effects on Earth.

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Publisher Correction: Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts

Abstract: A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

Cited by 2 publications

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Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats

Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats

Microgravity Effects on the Matrisome

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