Publisher Correction: Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

Fischer, Carola; Milting, Hendrik; Fein, Evelyn; Reiser, Elisabeth; Lu, Kun; Seidel, Thomas; Schinner, Camilla; Schwarzmayr, Thomas; Schramm, René; Tomasi, Roland; Husse, Britta; Cao-Ehlker, Xiaochun; Pohl, Ulrich; Dendorfer, Andreas

doi:10.1038/s41467-019-08510-9

Cited by 4 publications

(9 citation statements)

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“…During that same period, experiments with optical mapping of myocardial slices were performed (Kang et al, 2016). The most important breakthrough in LMS culture was achieved in 2019 when two groups developed novel technologies that prevented significant and functional changes in LMS associated with chronic in-vitro culture, now allowing for LMS cultivation of several weeks to months (Fischer et al, 2019;Watson et al, 2019). Since then the number of articles published on experiments utilizing myocardial slices increased considerably (Pitoulis et al, 2020b).…”

Section: Brief History Of Myocardial Slices In Electrophysiological R...mentioning

confidence: 99%

“…However, lack of mechanical loading of these slices still resulted in rapid decline of contractile force and altered cardiac expression invitro. In 2019, two groups presented novel biomimetic cultivation chambers that provide uniaxial mechanical loading and continuous electrical stimulation to LMS, improving cardiac phenotype and preserving contractile force of LMS (Fischer et al, 2019;Watson et al, 2019). Watson et al (2019) developed custom-made stretchers for LMS which were placed in culture chambers with continuous flow and oxygenation of the culture medium.…”

Section: Development Of Biomimetic Cultivation Chambersmentioning

confidence: 99%

“…This resulted in highly functional LMS for 5 days. Fischer et al (2019) choose a different approach with linear auxotonic loading of slices. Here, LMS were also mounted in a custom-made stretcher, but one end of the stretcher was able to move during contraction, resulting in elastic contractions.…”

Section: Development Of Biomimetic Cultivation Chambersmentioning

confidence: 99%

“…The technique to produce LMS has been described in detail (Brandenburger et al, 2011;Watson et al, 2017;Fischer et al, 2019;Watson et al, 2019;Watson et al, 2020;Hamers et al, 2022). In this review, we will focus on the technique to produce LMS from human (residual) tissue, which we believe best mimics human (electro-)physiology because of cellular and structural differences between humans and animals.…”

Section: Lms Techniquementioning

confidence: 99%

“…Recent technological advances enabled the transition of LMS towards a more representative model of the human myocardium (Bursac et al, 2003;Camelliti et al, 2011). A major step forward in the myocardial slicing technique was achieved by the introduction of special electromechanical cultivation chambers (Fischer et al, 2019;Watson et al, 2019), enabling long-term cultivation of LMS cultures. The aim of this review is to present the potential of a biomimetic interface using LMS in electrophysiological studies outlining the advantages, disadvantages and future perspectives of the interface.…”

Section: Introductionmentioning

confidence: 99%

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Living myocardial slices: Advancing arrhythmia research

Amesz

Zhang²,

Everts³

et al. 2023

Front. Physiol.

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Living myocardial slices (LMS) are ultrathin (150–400 µm) sections of intact myocardium that can be used as a comprehensive model for cardiac arrhythmia research. The recent introduction of biomimetic electromechanical cultivation chambers enables long-term cultivation and easy control of living myocardial slices culture conditions. The aim of this review is to present the potential of this biomimetic interface using living myocardial slices in electrophysiological studies outlining advantages, disadvantages and future perspectives of the model. Furthermore, different electrophysiological techniques and their application on living myocardial slices will be discussed. The developments of living myocardial slices in electrophysiology research will hopefully lead to future breakthroughs in the understanding of cardiac arrhythmia mechanisms and the development of novel therapeutic options.

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Section: Brief History Of Myocardial Slices In Electrophysiological R...mentioning

confidence: 99%

Section: Development Of Biomimetic Cultivation Chambersmentioning

confidence: 99%

Section: Development Of Biomimetic Cultivation Chambersmentioning

confidence: 99%

Section: Lms Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Living myocardial slices: Advancing arrhythmia research

Amesz

Zhang²,

Everts³

et al. 2023

Front. Physiol.

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Myocardial slices come to age: an intermediate complexity in vitro cardiac model for translational research

Pitoulis

Watson

Perbellini

et al. 2019

Cardiovascular Research

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Although past decades have witnessed significant reductions in mortality of heart failure together with advances in our understanding of its cellular, molecular, and whole-heart features, a lot of basic cardiac research still fails to translate into clinical practice. In this review we examine myocardial slices, a novel model in the translational arena. Myocardial slices are living ultra-thin sections of heart tissue. Slices maintain the myocardium’s native function (contractility, electrophysiology) and structure (multicellularity, extracellular matrix) and can be prepared from animal and human tissue. The discussion begins with the history and current advances in the model, the different interlaboratory methods of preparation and their potential impact on results. We then contextualize slices’ advantages and limitations by comparing it with other cardiac models. Recently, sophisticated methods have enabled slices to be cultured chronically in vitro while preserving the functional and structural phenotype. This is more timely now than ever where chronic physiologically relevant in vitro platforms for assessment of therapeutic strategies are urgently needed. We interrogate the technological developments that have permitted this, their limitations, and future directions. Finally, we look into the general obstacles faced by the translational field, and how implementation of research systems utilizing slices could help in resolving these.

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Inhibition of miR-21: cardioprotective effects in human failing myocardium ex vivo

Abbas,

Haas,

Xiao

et al. 2024

European Heart Journal

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Publisher Correction: Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

Abstract: The original version of this Article incorrectly acknowledged Elisabeth Reiser and Rene Schramm as a corresponding author. This has now been corrected in both the PDF and HTML versions of the Article.

Cited by 4 publications

References 0 publications

Living myocardial slices: Advancing arrhythmia research

Living myocardial slices: Advancing arrhythmia research

Myocardial slices come to age: an intermediate complexity in vitro cardiac model for translational research

Inhibition of miR-21: cardioprotective effects in human failing myocardium ex vivo

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