Publisher Correction: Human heart-forming organoids recapitulate early heart and foregut development

Drakhlis, Lika; Biswanath, Santoshi; Farr, Clara-Milena; Lupanow, Victoria; Teske, Jana; Ritzenhoff, Katharina; Franke, Annika; Manstein, Felix; Bolesani, Emiliano; Kempf, Henning; Liebscher, Simone; Schenke‐Layland, Katja; Hegermann, Jan; Nolte, Lena; Meyer, Heiko; Thiemann, Stefan; Wahl‐Schott, Christian; Zweigerdt, Robert

doi:10.1038/s41587-021-00960-1

Cited by 13 publications

(1 citation statement)

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“…As for the cell source, Induced Pluripotent Stem Cells (iPSCs) are a powerful resource due to their similarity to Embryonic Stem Cells and their ability to differentiate towards various cell lines and tissue types. Over the past 17 years, iPSCs have been extensively studied for medical purposes, including disease modelling, drug screening, and regenerative medicine [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Several studies, some of which have advanced to clinical trials, have shown that iPSCs have the potential to generate functional tissues [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation on Electrospun and Solvent-Casted PCL-PLGA Blends Scaffolds Embedded with Induced Pluripotent Stem Cells for Tissue Engineering

Rosalia,

Giacomini,

Tottoli

et al. 2023

Pharmaceutics

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The design, production, and characterisation of tissue-engineered scaffolds made of polylactic-co-glycolic acid (PLGA), polycaprolactone (PCL) and their blends obtained through electrospinning (ES) or solvent casting/particulate leaching (SC) manufacturing techniques are presented here. The polymer blend composition was chosen to always obtain a prevalence of one of the two polymers, in order to investigate the contribution of the less concentrated polymer on the scaffolds’ properties. Physical–chemical characterization of ES scaffolds demonstrated that tailoring of fibre diameter and Young modulus (YM) was possible by controlling PCL concentration in PLGA-based blends, increasing the fibre diameter from 0.6 to 1.0 µm and reducing the YM from about 22 to 9 MPa. SC scaffolds showed a “bubble-like” topography, caused by the porogen spherical particles, which is responsible for decreasing the contact angles from about 110° in ES scaffolds to about 74° in SC specimens. Nevertheless, due to phase separation within the blend, solvent-casted samples displayed less reproducible properties. Furthermore, ES samples were characterised by 10-fold higher water uptake than SC scaffolds. The scaffolds suitability as iPSCs culturing support was evaluated using XTT assay, and pluripotency and integrin gene expression were investigated using RT-PCR and RT-qPCR. Thanks to their higher wettability and appropriate YM, SC scaffolds seemed to be superior in ensuring high cell viability over 5 days, whereas the ability to maintain iPSCs pluripotency status was found to be similar for ES and SC scaffolds.

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