Publisher Correction: Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells

Caires-Júnior, Luiz C.; Goulart, Ernesto; Melo, Uirá Souto; Araújo, Bruno Henrique Silva; Alvizi, Lucas; Soares‐Schanoski, Alessandra; Oliveira, Danyllo; Kobayashi, Gerson Shigeru; Griesi-Oliveira, Karina; Musso, Camila Manso; Amaral, Murilo Sena; DaSilva, Luis; Astray, Renato Mancini; Suárez-Patiño, Sandra Fernanda; Ventini, Daniella C.; Silva, Sérgio Gomes da; Yamamoto, Guilherme Lopes; Ezquina, Suzana; Naslavsky, Michel Satya; Telles-Silva, Kayque A.; Weinmann, Karina; Linden, Vanessa van der; Linden, Hélio van der; Oliveira, João Ricardo Mendes de; Arrais, Nivia Maria Rodrigues; Melo, A.O.; Figueiredo, Thalita; Santos, Silvana; Meira, Joanna Goes Castro; Passos, Saulo Duarte; Almeida, Roque Pacheco de; Bispo, Ana Jovina Barreto; Cavalheiro, Ésper A.; Kalil, Jorge; Cunha‐Neto, Edécio; Nakaya, Helder I.; Andreata-Santos, Robert; Ferreira, Luís Carlos de Souza; Verjovski-Almeida, Sérgio; Ho, Paulo Lee; Passos‐Bueno, Maria Rita; Zatz, Mayana

doi:10.1038/s41467-018-03497-1

Cited by 7 publications

(5 citation statements)

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“…Congenital ZIKV infection was recognized in 2016 as a teratogenic event, that is, capable of affecting the development of foetuses and embryos by causing congenital anomalies. However, it has been reported over the years that, despite the severe consequences of the congenital infection, some exposed individuals did not develop any alteration (Caires-Júnior et al, 2018 ; Pomar et al, 2019 ; Vhp et al, 2020 ). This suggests that genetic risk factors may act on this differential susceptibility for the development of CZS.…”

Section: Resultsmentioning

confidence: 99%

“…Studies with animal models have shown, for example, that inefficient binding of the ZIKV NS5 protein to mouse Stat2 renders the virus unable to antagonize the mouse IFN signalling, which ensures the species’ resistance to the infection and adverse outcomes (Grant et al, 2016 ; Gorman et al, 2018 ). On the other hand, genetic susceptibility to CZS in humans has been investigated through the presence of pathogenic variants in genes related to CZS phenotypes, or related to neurodevelopment and immune response pathways in affected individuals (Caires-Júnior et al, 2018 ; Aguiar et al, 2020 ; Borda et al, 2021 ; Gomes et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…In order to understand how congenital infection impairs neurodevelopment, some researchers have investigated the molecular changes induced by ZIKV in neural cells (Rolfe et al, 2016 ; Caires-Júnior et al, 2018 ). In their studies, infection in neural cells has been shown to disrupt the expression of several microRNAs (miRNAs) (Azouz et al, 2019 ; Dang et al, 2019 ), which are post-transcriptional regulators, acting through degradation and/or translational repression of the messenger RNAs (mRNAs) of their target genes (Catalanotto et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Contribution of miR-124 rs531564 polymorphism to the occurrence of congenital Zika syndrome

et al. 2022

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Zika virus (ZIKV) cause Congenital Zika Syndrome (CZS) in individuals exposed during pregnancy. Studies have shown that ZIKV infection positively regulates the miR-124 expression in neural cells, which leads to a decrease of TFRC, a gene targeted of this miRNA. Both miR-124 and TFRC exhibit a pivotal role in nervous system development. Therefore, in this study we aimed to investigate whether genetic variants that affect the expression of these genes could act together with ZIKV to increase the risk of individuals developing CZS. TFRC rs406271 and MIR-124-1 rs531564 polymorphisms were genotyped, using TaqMan® Genotyping Assays, in a sample of children who were exposed to ZIKV during pregnancy, of whom 40 were born with CZS and 48 without congenital anomalies. We identified that individuals with CZS presented a higher frequency of CG genotype of rs531564 polymorphism in MIR-124-1 (p=0.048), which is associated with increased expression of miR-124. Since ZIKV also upregulates the expression of this miRNA, the presence of CG genotype in individuals exposed to the virus could lead to a scenario of overexpression of miR-124 in the brain. Since teratogenesis is a multifactorial event, this genetic finding could partly explain why such individuals are more susceptible to CZS, considering both the downregulation of important neurodevelopment genes, as well as deregulation of the neurogenesis process. Thus, we provide preliminary evidence about a possible genetic risk factor to CZS and highlight the importance of analyzing functional polymorphisms related to epigenetic modulators of neurodevelopment genes in the context of ZIKV teratogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Contribution of miR-124 rs531564 polymorphism to the occurrence of congenital Zika syndrome

et al. 2022

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“…Nonetheless, the conserved sequences shared by DENV and ZIKV NS1 result in high cross-reactivity of antibodies raised in infected patients. A demonstration that an antigen based on C-terminal NS1 epitopes could confer higher specificity to antibody detection represented an important step toward the development of improved serological tests capable of differentiating ZIKV and DENV infections [ 14 , 24 ]. The development of a recombinant chimeric DENV NS1 antigen preserved the high specific serological detection based on the use of a single antigen and, therefore, avoided the demand to generate four antigens of each DENV serotype.…”

Section: Discussionmentioning

confidence: 99%

Multi-epitope Antigen for Specific Serological Detection of Dengue Viruses

Pereira,

Andreata-Santos,

de Castro-Amarante

et al. 2023

Viruses

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Dengue is an infectious disease of global health concern that continues to require surveillance. Serological testing has been used to investigate dengue-infected patients, but specificity is affected by the co-circulation of ZIKA virus (ZIKV), which shares extensive antigen similarities. The goal of this study was the development of a specific dengue virus (DENV) IgG ELISA based on a multi-epitope NS1-based antigen for antibody detection. The multi-epitope protein (T-ΔNS1), derived from a fragment of the NS1-protein of the four DENV serotypes, was expressed in Escherichia coli and purified via affinity chromatography. The antigenicity and specificity were evaluated with sera of mice infected with DENV-1–4 or ZIKV or after immunization with the recombinant ΔNS1 proteins. The performance of the T-ΔNS1-based IgG ELISA was also determined with human serum samples. The results demonstrate that the DENV T-ΔNS1 was specifically recognized by the serum IgG of dengue-infected mice or humans but showed no or reduced reactivity with ZIKV-infected subjects. Based on the available set of clinical samples, the ELISA based on the DENV T-ΔNS1 achieved 77.42% sensitivity and 88.57% specificity. The results indicate that the T-ΔNS1 antigen is a promising candidate for the development of specific serological analysis.

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“…is ≤0.217. This kit was chosen because of its high specificity for the detection of anti-Zika antibodies, even in dengue endemic areas[18][19][20][21][22][23][24][25]. Table1shows sensitivity, specificity, positive…”

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confidence: 99%

Seroprevalence of Zika in Brazil stratified by age and geographic distribution

Botosso,

Precioso,

Wilder-Smith

et al. 2023

Epidemiol. Infect.

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Congenital Zika is a devastating consequence of maternal Zika virus infections. Estimates of agedependent seroprevalence profiles are central to our understanding of the force of Zika virus infections. We set out to calculate the age-dependent seroprevalence of Zika virus infections in Brazil. We analyzed serum samples stratified by age and geographic location, collected from 2016 to 2019, from about 16,000 volunteers enrolled in a Phase 3 dengue vaccine trial led by the Institute Butantan in Brazil. Our results show that Zika seroprevalence has a remarkable agedependent and geographical distribution, with an average age of the first infection varying from region to region, ranging from 4.97 (3.03-5.41) to 7.24 (6.98-7.90) years. The calculated basic reproduction number, R 0 , varied from region to region, ranging from 1.18 (1.04-1.41) to 2.33 (1.54-3.85). Such data are paramount to determine the optimal age to vaccinate against Zika, if and when such a vaccine becomes available.

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Publisher Correction: Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells

Cited by 7 publications

References 0 publications

Contribution of miR-124 rs531564 polymorphism to the occurrence of congenital Zika syndrome

Contribution of miR-124 rs531564 polymorphism to the occurrence of congenital Zika syndrome

Multi-epitope Antigen for Specific Serological Detection of Dengue Viruses

Seroprevalence of Zika in Brazil stratified by age and geographic distribution

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