Public Health Program for Decreasing Risk for Ebola Virus Disease Resurgence from Survivors of the 2013–2016 Outbreak, Guinea

Keïta, Mory; Keïta, Sakoba; Diallo, Boubacar; Camara, Momo; Mesfin, Samuel; Nébié, Koumpingnin; Magassouba, N’Faly; Coulibaly, Seydou; Barry, Boubacar; Baldé, Mamadou Oury; Pallawo, Raymond; Sow, Sadou; Diallo, Amadou; Formenty, Pierre; Djingarey, Mamoudou Harouna; Fall, Ibrahima Socé; Subissi, Lorenzo

doi:10.3201/eid2602.191235

Cited by 6 publications

(8 citation statements)

References 22 publications

(21 reference statements)

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“…The continuous threat of Ebola Virus Disease is manifest in a new outbreak in Guinea, beginning January 18, 2021 [ 8 ]. Virus sequences posted to a pre-print sequence site March 12, 2021 indicate the infections are a resurgence of the West African outbreak of 2013–2016 [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Rapid identification of the Filovirus in the Democratic Republic of the Congo [ 16 ] as EBOV by Next Generation Sequencing (NGS) supported the use of a rVSV-ZEBOV-GP vaccine which was important in controlling that outbreak. The need for rapid, accessible sequence data is indicated by the identification of the variant in the most recent outbreak in Guinea as a resurgence of the earlier outbreak thought to have ended in 2016 [ 9 , 10 ]. Together, these studies demonstrate the value of sequencing methods that can be located close to the outbreak to quickly generate real-time sequencing data without the need for a large bioinformatics infrastructure.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tracking ebolavirus genomic drift with a resequencing microarray

Tiper

Kourout

Lanning³

et al. 2022

PLoS ONE

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Filoviruses are emerging pathogens that cause acute fever with high fatality rate and present a global public health threat. During the 2013–2016 Ebola virus outbreak, genome sequencing allowed the study of virus evolution, mutations affecting pathogenicity and infectivity, and tracing the viral spread. In 2018, early sequence identification of the Ebolavirus as EBOV in the Democratic Republic of the Congo supported the use of an Ebola virus vaccine. However, field-deployable sequencing methods are needed to enable a rapid public health response. Resequencing microarrays (RMA) are a targeted method to obtain genomic sequence on clinical specimens rapidly, and sensitively, overcoming the need for extensive bioinformatic analysis. This study presents the design and initial evaluation of an ebolavirus resequencing microarray (Ebolavirus-RMA) system for sequencing the major genomic regions of four Ebolaviruses that cause disease in humans. The design of the Ebolavirus-RMA system is described and evaluated by sequencing repository samples of three Ebolaviruses and two EBOV variants. The ability of the system to identify genetic drift in a replicating virus was achieved by sequencing the ebolavirus glycoprotein gene in a recombinant virus cultured under pressure from a neutralizing antibody. Comparison of the Ebolavirus-RMA results to the Genbank database sequence file with the accession number given for the source RNA and Ebolavirus-RMA results compared to Next Generation Sequence results of the same RNA samples showed up to 99% agreement.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tracking ebolavirus genomic drift with a resequencing microarray

Tiper

Kourout

Lanning³

et al. 2022

PLoS ONE

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“…As this cluster was detected within 42 days of the last EVD case, all activities were under continuation: alerts, alert investigation, etc., and the index case was detected within a reasonable time. Additionally, following up EVD survivors and sensitizing and vaccinating their close contacts are important strategies to prevent future outbreaks [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Investigation of and Strategies to Control the Final Cluster of the 2018–2020 Ebola Virus Disease Outbreak in the Eastern Democratic Republic of Congo

Keïta

Polonsky

Finci

et al. 2022

Open Forum Infectious Diseases

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Background On April 10, 2020, while the independent committee of the International Health Regulation was meeting to decide whether the 10th Ebola outbreak in the Demogratic Republic of Congo still constituted a Public Health Emergency of International Concern, a new confirmed case was reported in the city of Beni, the last epicenter of the epidemic. This study aimed to understand the source of this cluster and learn from the implemented control strategies for improved response in the future. Methods We conducted a combined epidemiological and genomic investigation to understand the origins and dynamics of transmission within this cluster and describe the strategy that successfully controlled the outbreak. Results Eight cases were identified as belonging to this final cluster. A total of 1028 contacts were identified. Whole-genome sequencing revealed that all cases belonged to the same cluster, the closest sequence to which was identified as a case from the Beni area with symptom onset in July 2019 and a difference of just 31 nucleotides. Outbreak control measures included community confinement of high-risk contacts. Conclusions This study illustrates the high risk of additional flare-ups in the period leading to the end-of-outbreak declaration and the importance of maintaining enhanced surveillance and confinement activities to rapidly control Ebola outbreaks.

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“…Three studies reported outcomes of the same prospective cohort (PostEbogui) study of EBOV disease survivors. 26,63,64 We considered the breast milk findings from this cohort to correspond to the same samples in all articles and thus, we only describe the findings reported by Keita and collaborators. 63 A PRISMA flow diagram of the systematic review search data is presented in Figure 1.…”

Section: Study Designsmentioning

confidence: 99%

“…We found a total of 13 records for data extraction and descriptive analysis. Among these, four studies were case reports, [57][58][59][60] three records reported on outbreak surveillance efforts, 16,61,62 three records reported on a prospective observational study of the same cohort of EBOV disease survivors, 26,63,64 one study was a methodology report of a cohort, 65 and two records were conference abstracts. 66,67 For the methodology report and conference abstracts, [65][66][67] no details of the population of interest or collected breast milk samples were found.…”

Section: Study Designsmentioning

confidence: 99%

Presence of Ebola virus in breast milk and risk of mother‐to‐child transmission: synthesis of evidence

Medina‐Rivera

Centeno‐Tablante

Finkelstein

et al. 2020

Annals of the New York Academy of Sciences

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To help inform global guidelines on infant feeding, this systematic review synthesizes evidence related to the presence of the Ebola virus (EBOV) in breast milk and its potential risk of viral transmission to the infant when breastfeeding. We relied on a comprehensive search strategy to identify studies including women with suspected, probable, or confirmed EBOV infection, intending to breastfeed or give breast milk to an infant. Our search identified 10,454 records, and after deduplication and screening, we assessed 148 full texts. We included eight studies reporting on 10 breastfeeding mothers and their children (one mother with twins), who provided breast milk samples for assessment. EBOV was detected via RT-PCR or viral culture in seven out of ten breast milk samples. Four out of the five-breastfed infants with EBOV-positive breast milk were found positive for EBOV infection, and all of these EBOV-positive infants died. Since previous reports have detected EBOV in tears, saliva, sweat, and contaminated surfaces, with the current evidence, it is not possible to conclude with certainty that breast milk was the main route of EBOV transmission.

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Public Health Program for Decreasing Risk for Ebola Virus Disease Resurgence from Survivors of the 2013–2016 Outbreak, Guinea

Cited by 6 publications

References 22 publications

Tracking ebolavirus genomic drift with a resequencing microarray

Tracking ebolavirus genomic drift with a resequencing microarray

Investigation of and Strategies to Control the Final Cluster of the 2018–2020 Ebola Virus Disease Outbreak in the Eastern Democratic Republic of Congo

Presence of Ebola virus in breast milk and risk of mother‐to‐child transmission: synthesis of evidence

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