PU.1-Regulated Long Noncoding RNA lnc-MC Controls Human Monocyte/Macrophage Differentiation through Interaction with MicroRNA 199a-5p

Chen, Mingtai; Lin, Haishuang; Shen, Chao; Ma, Yupo; Wang, Fang; Zhao, Hua-Lu; Yu, Jia; Zhang, Jun-Wu

doi:10.1128/mcb.00429-15

Cited by 98 publications

(73 citation statements)

References 52 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The differentiation of monocyte/macrophage is controlled by a complicated process including the coordinated expression of stage-specific transcription factors, various cytokines and ncRNAs 67,68) . Chen et al 69) found that PU.1-regulated two ncRNAs, lncRNA monocyte (lnc-MC) and miR-199a-5p, which have opposing roles during the differentiation of monocyte/ macrophage. Lnc-MC could promote monocyte/macrophage differentiation through function as a miR199a-5p sponge and decrease repression on ACVR1B (activin A receptor type 1B) expression, which was an important regulator of monocyte/macrophage differentiation.…”

Section: Long Noncoding Rnas and Monocyte/ Macrophagesmentioning

confidence: 99%

Long Noncoding RNAs in Atherosclerosis

Jian

Chen

et al. 2016

JAT

View full text Add to dashboard Cite

Section: Long Noncoding Rnas and Monocyte/ Macrophagesmentioning

confidence: 99%

Long Noncoding RNAs in Atherosclerosis

Jian

Chen

et al. 2016

JAT

View full text Add to dashboard Cite

“…Increasing literatures have confirmed that ceRNAs play key roles in many biological processes and immune responses [34,35], and miRNAs have been documented to be involved in the regulation of macrophage polarization [36,37] , similar to lncRNAs [14][15][16]. We were interested in identifying whether an mRNA-related ceRNA network existed during F. gigantica ESP-induced M2 cell polarization into M1 cells.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, knocking down TCONS_00019715 following the activation of THP-1 cells using IFN-γ and LPS diminished the expression of M1 markers and elevated the expression of M2 markers [16]. Another lncRNA, lnc-MC, acted as a ceRNA to miR-199a-5p and alleviated repression of activin A receptor type 1B (ACVR1B), a regulator of monocyte/macrophage polarization [14]. In our study, 1, 579 and 1, 265 lncRNAs and 789 and 993 mRNAs were upregulated and downregulated, respectively, in Fasciola ESPinduced M2 cells differentiated into IFN-γ + LPS-induced M1 cells.…”

Section: Discussionmentioning

confidence: 99%

“…We hypothesized that the identification of molecular targets that can regulate the M1 and M2 switching, may be important in discovering ways in which F.gigantica infections may be controled by genetic engineering. LncRNAs have been shown to play important roles in macrophage polarization and activation [14][15][16]; therefore, we explored whether lncRNAs extended this function to F. gigantica ESP-induced macrophage polarization. In our study, immunological reactions in which mice responded to ESP stimulation in vivo were simulated, and we detected the differentially expressed lncRNAs and mRNAs in F. gigantica ESP-induced M2 cells and compared these with IFN-γ + LPS-induced M1 cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Long Noncoding RNA Profiling from Fasciola Gigantica Excretory/Secretory Product-Induced M2 to M1 Macrophage Polarization

Luo

Zhang

Sheng

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Long noncoding RNAs (lncRNAs) are well known regulators of gene expression that play essential roles in macrophage activation and polarization. However, the role of lncRNA in Fasciola gigantica excretory/secretory products (ESP)-induced M2 polarization into M1 macrophages is unclear. Herein, we performed lncRNA profiling of lncRNAs and mRNAs during the ESP-induced macrophage polarization process. Methods: F. gigantica ESP was used to induce peritoneal cavity M2 macrophages in BALB/c mice (5-6 weeks old) in vivo, and these cells were subsequently isolated and stimulated with IFN-γ + LPS to induce M1 cells in vitro. LncRNA and mRNA profiling was performed via microarray at the end of both polarization stages. Results: In total, 2,844 lncRNAs (1,579 upregulated and 1,265 downregulated) and 1,782 mRNAs (789 upregulated and 993 downregulated) were differentially expressed in M2 macrophages compared to M1 macrophages, and six lncRNAs were identified during polarization. We selected 34 differentially expressed lncRNAs and mRNAs to validate the results of microarray analysis using quantitative real-time PCR (qPCR). Pathway and Gene Ontology (GO) analyses demonstrated that these altered transcripts were involved in multiple biological processes, particularly peptidase activity and carbohydrate metabolism. Furthermore, coding and non-coding gene (CNC) and mRNA-related ceRNA network analyses were conducted to predict lncRNA expression trends and the potential target genes of these lncRNAs and mRNAs. Moreover, we determined that four lncRNAs and four mRNAs might participate in F. gigantica ESP-induced M2 polarization into M1 macrophages. Conclusions: This study illustrates the basic profiling of lncRNAs and mRNAs during F. gigantica ESP-induced M2 polarization into M1 macrophages and deepens our understanding of the mechanism underlying this process.

show abstract

“…Monocyte/macrophage differentiation is controlled by a complex process and requires cooperative expression of the stage-specific transcription factors, various cytokines, and ncRNAs. Chen et al (43) reported two ncRNAs: lncRNA monocyte (lnc-MC) and miR-199a-5p, which are regulated by PU.1 with opposite roles during monocyte/macrophage differentiation. Lnc-MC acts as a sponge for miR-199a-5p and promotes monocyte/macrophage differentiation, and it can reduce the suppression on the expression of activin receptor type 1B (ACVR1B), which is an important monocyte/macrophage differentiation regulator.…”

Section: Importance Of Lncrnas In the Cells Involved In The Formationmentioning

confidence: 99%

The Roles of the Long Non-Coding RNA Transcripts in Cardiovascular Diseases

Bayoğlu¹,

Cengiz²

2017

Bezmialem Science

View full text Add to dashboard Cite

For many years, proteins have been known be the major regulators in biological processes such as transcription and translation. However, in recent years, with the advent of high-throughput sequencing technology, a high-resolution map of the human transcriptome was made and a large number of non-protein coding RNA genes were discovered. Non-protein coding RNA genes (ncRNAs) are classified according to their size. Long non-coding RNAs (lncRNAs) are a group of ncRNAs that are >200 nucleotides long. LncRNAs are involved in many biological processes. Expression levels of and genetic variations in lncRNAs contribute to in vivo and in vitro pathophysiological processes and have been associated with many diseases. In recent years, numerous lncRNAs, which are associated with cardiovascular disease (CVD), have been identified. One of the major causes of CVD is atherosclerosis. The molecular processes involved in the formation and progression of atherosclerosis in vascular smooth muscle cells, endothelial cells, and monocytes/macrophages play an important role in the development of CVD. In the future, a better understanding of the biological functions of lncRNAs with their ever-increasing importance in the formation and development of atherosclerosis will shed light on the development of novel therapeutic approaches for CVD.

show abstract

PU.1-Regulated Long Noncoding RNA lnc-MC Controls Human Monocyte/Macrophage Differentiation through Interaction with MicroRNA 199a-5p

Cited by 98 publications

References 52 publications

Long Noncoding RNAs in Atherosclerosis

Long Noncoding RNAs in Atherosclerosis

Long Noncoding RNA Profiling from Fasciola Gigantica Excretory/Secretory Product-Induced M2 to M1 Macrophage Polarization

The Roles of the Long Non-Coding RNA Transcripts in Cardiovascular Diseases

Contact Info

Product

Resources

About