Ptpn20 deletion in H-Tx rats enhances phosphorylation of the NKCC1 cotransporter in the choroid plexus: an evidence of genetic risk for hydrocephalus in an experimental study

Miyajima, Masakazu; Nakajima, Madoka; Ogino, Ikuko; Kawamura, Kaito; Akiba, Chihiro; Kamohara, Chihiro; Sakamoto, Koichiro; Karagiozov, Kostadin; Nakamura, Eri; Tada, Norio; Arai, Hajime; Kondo, Akihide

doi:10.1186/s12987-022-00341-z

Cited by 6 publications

(5 citation statements)

References 78 publications

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“…This result is in consistent with the idea that PTPN20 participates in the regulation of cell skeleton structure and movement (34). In hydrocephalic H-Tx rat, knocking out the Ptpn20 gene increases the level of phosphorylated NKCC1, which is a Na-K-Cl cotransporter (37). This may cause overproduction of cerebrospinal fluid and lead to the development of hydrocephalus.…”

Section: Discussionmentioning

confidence: 99%

Identification of PTPN20 as an innate immunity-related gene in gastric cancer with Helicobacter pylori infection

Liu

Wang

et al. 2023

Front. Immunol.

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BackgroundGastric cancer (GC) is among the deadliest diseases with countless incidences and deaths each year. Helicobacter pylori (Hp) is the primary type of microbe that colonizes the stomach. In recent years, increasing evidence has demonstrated that Hp infection is one of the main risk factors for GC. Elucidating the molecular mechanism of how Hp leads to GC will not only benefit the treatment of GC, but also boost the development of therapeutics for other gastric disorders caused by Hp infection. In this study, we aimed to identify innate immunity-related genes in GC and investigate their potentials as prognostic markers and therapeutic targets for Hp-related GC.MethodsFirstly, we analyzed the differentially expressed innate immunity-related genes in GC samples from the TCGA database. Then prognostic correlation analysis was carried out to explore the prognostic value of these candidate genes. By combing transcriptome data, somatic mutation data, and clinical data, co-expression analysis, functional enrichment analysis, tumor mutational burden analysis, and immune infiltration analysis were performed to reveal the pathological relevance of the candidate gene. Finally, ceRNA network was constructed to identify the genes and pathways for the regulation of the candidate gene.ResultsWe revealed that protein tyrosine phosphatase non-receptor type 20 (PTPN20) is a significant prognostic marker in Hp-related GC. Thus, PTPN20 levels have the potential to efficiently predict the survival of Hp-related GC patients. In addition, PTPN20 is associated with immune cell infiltration and tumor mutation burden in these GC patients. Moreover, we have also identified PTPN20-related genes, PTPN20 protein-protein interactions, and the PTPN20 ceRNA network.ConclusionOur data suggest that PTPN20 may have critical functions in Hp-related GC. Targeting PTPN20 may be a promising way to treat Hp-related GC.

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Section: Discussionmentioning

confidence: 99%

Identification of PTPN20 as an innate immunity-related gene in gastric cancer with Helicobacter pylori infection

Liu

Wang

et al. 2023

Front. Immunol.

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“…Non-receptor protein tyrosine phosphatases (PTPNs), involved in peptide-tyrosine dephosphorylation, regulate protein tyrosine phosphatase activity. Deletion of the ptpn20 gene in the PTPNs family leads to enhanced phosphorylation of NKCC1 cotransporters, thereby increasing CSF secretion and resulting in hydrocephalus [ 92 , 93 ].…”

Section: Mechanism Classification Of Chmentioning

confidence: 99%

A Review of Cerebrospinal Fluid Circulation and the Pathogenesis of Congenital Hydrocephalus

Zhang,

Hu,

Wang

2024

Neurochem Res

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The brain’s ventricles are filled with a colorless fluid known as cerebrospinal fluid (CSF). When there is an excessive accumulation of CSF in the ventricles, it can result in high intracranial pressure, ventricular enlargement, and compression of the surrounding brain tissue, leading to potential damage. This condition is referred to as hydrocephalus. Hydrocephalus is classified into two categories: congenital and acquired. Congenital hydrocephalus (CH) poses significant challenges for affected children and their families, particularly in resource-poor countries. Recognizing the psychological and economic impacts is crucial for developing interventions and support systems that can help alleviate the distress and burden faced by these families. As our understanding of CSF production and circulation improves, we are gaining clearer insights into the causes of CH. In this article, we will summarize the current knowledge regarding CSF circulation pathways and the underlying causes of CH. The main causes of CH include abnormalities in the FoxJ1 pathway of ventricular cilia, dysfunctions in the choroid plexus transporter Na+-K+-2Cl- contransporter isoform 1, developmental abnormalities in the cerebral cortex, and structural abnormalities within the brain. Understanding the causes of CH is indeed crucial for advancing research and developing effective treatment strategies. In this review, we will summarize the findings from existing studies on the causes of CH and propose potential research directions to further our understanding of this condition.

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“…These cells release cytokines, such as IL-6, IL-12, IL-8, and TNF, that stimulate type 1 T-helper cells [131]. PTPN20 deletion in the choroid plexus epithelium was shown to cause an overproduction of cerebrospinal fluid, contributing to the formation of hydrocephalus [132]. PTPN20 was also listed among the 10 proteins with the highest mean autoantibody response in COVID-19 patients [133].…”

Section: Genes/proteins Identified By 1-l Transcription Of C-(aa)n-n ...mentioning

confidence: 99%

1-L Transcription of SARS-CoV-2 Spike Protein S1 Subunit

Nahalka

2024

IJMS

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The COVID-19 pandemic prompted rapid research on SARS-CoV-2 pathogenicity. Consequently, new data can be used to advance the molecular understanding of SARS-CoV-2 infection. The present bioinformatics study discusses the “spikeopathy” at the molecular level and focuses on the possible post-transcriptional regulation of the SARS-CoV-2 spike protein S1 subunit in the host cell/tissue. A theoretical protein–RNA recognition code was used to check the compatibility of the SARS-CoV-2 spike protein S1 subunit with mRNAs in the human transcriptome (1-L transcription). The principle for this method is elucidated on the defined RNA binding protein GEMIN5 (gem nuclear organelle-associated protein 5) and RNU2-1 (U2 spliceosomal RNA). Using the method described here, it was shown that 45% of the genes/proteins identified by 1-L transcription of the SARS-CoV-2 spike protein S1 subunit are directly linked to COVID-19, 39% are indirectly linked to COVID-19, and 16% cannot currently be associated with COVID-19. The identified genes/proteins are associated with stroke, diabetes, and cardiac injury.

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Ptpn20 deletion in H-Tx rats enhances phosphorylation of the NKCC1 cotransporter in the choroid plexus: an evidence of genetic risk for hydrocephalus in an experimental study

Cited by 6 publications

References 78 publications

Identification of PTPN20 as an innate immunity-related gene in gastric cancer with Helicobacter pylori infection

Identification of PTPN20 as an innate immunity-related gene in gastric cancer with Helicobacter pylori infection

A Review of Cerebrospinal Fluid Circulation and the Pathogenesis of Congenital Hydrocephalus

1-L Transcription of SARS-CoV-2 Spike Protein S1 Subunit

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