PTHrP regulation and calcium balance in sea bream (<i>Sparus auratus</i>L.) under calcium constraint

Abbink, W.; Bevelander, Gideon S.; Hang, Xiaoming; Lu, Wei; Guerreiro, Pedro M.; Spanings, Tom; Canário, Adelino V.M.; Flik, G.

doi:10.1242/jeb.02399

Cited by 25 publications

(17 citation statements)

References 36 publications

(30 reference statements)

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“…This widespread distribution of the pthrp gene and protein reflects the wide range of functions that are reported for PTHrP in fish and suggests endocrine, paracrine, autocrine and intracrine functions; at this moment, no reports on intracrine effects in fish tissues are available. The (high) cir culating PTHrP-levels in the range of other protein endoc rines (nmol l_1) would be in line with a classical endocrine function for (pituitary) PTHrP (Danks et al, 1993) as is its presence in pituitary gland cells Abbink et al, 2006).…”

Section: Pthrp In Teleostsmentioning

confidence: 57%

“…PTHrP was quantified in plasma by radioimmunoassay using heterologous antisera raised against human peptide (Danks et al, 1993;Devlin et al, 1996) and later with homologous antisera (Rotllant et al, 2003;Abbink et al, 2004Abbink et al, , 2006. As holds for elasmobranch species, PTHrP levels in teleostean plasma are consistently and significantly higher than these values for humans (basal circulating lev els of around 0.5-2.5 pmol l_1 in humans and 0.1 0.6 nmol l_1 in fish).…”

Section: Pthrp In Teleostsmentioning

confidence: 99%

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Parathyroid hormone-related protein in teleost fish

Abbink

Flik

2007

General and Comparative Endocrinology

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A brief description is given of the discovery of PTHrP and the roles of the peptide in mammalian physiology. Next, the occurrence of PTHrP in the earliest vertebrates, sharks, skates and fishes, is reviewed and the calciotropic functions of PTHrP are addressed more spe cifically in fishes. Parathyroid hormone-related protein (PTHrP) is a hypercalcemic hormone in teleostean fishes, but also has para-and autocrine functions. After the isolation and identification of fish PTHrP and PTHrP receptors and the subsequent development of recom binant protein and a real-time quantitative PCR, a calciotropic role of PTHrP in fish physiology could be assessed. PTHrP influences calcium physiology via regulation of calcium mobilisation from internal sources (bone and scales) and via calcium uptake from the envi ronment (water and diet). Continuous variations in the need for calcium and in the availability of environmental calcium require fast calciotropes to guarantee calcium balance, in which PTHrP is pivotal for the fish. PTHrP is essential in fish bone physiology, e.g. in min eralisation and calcium reabsorption from the scales. Moreover, PTHrP plays a role in vitellogenesis, cortisol production, regulation of renal Mrp2 activity and melatonin synthesis. The plethora of functions of PTHrP in fish concern endocrine, paracrine and autocrine (and possibly intracrine) functions; calciotropic actions of PTHrP at the organismal and cellular level are prominent in fish. The strong con servation of the pthrp gene in the vertebrate lineage and the N-terminal similarity of the coded proteins relates to the important role of PTHrP in calcium physiology that is of paramount importance to all physiological processes. Recent and ongoing studies will contribute to our rapidly expanding knowledge of the original physiological functions of PTHrP in teleost fish.

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Section: Pthrp In Teleostsmentioning

confidence: 57%

Section: Pthrp In Teleostsmentioning

confidence: 99%

Parathyroid hormone-related protein in teleost fish

Abbink

Flik

2007

General and Comparative Endocrinology

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“…PTHrP behaves in fish as a hypercalcemic hormone and appears key in fish calcium physiology (Ingleton et al, 2002;Trivett et al, 2001). Recently, we established a strict relationship between PTHrP levels in plasma and plasma Ca2+ in juvenile sea bream (Abbink et al, 2006). PTHrP is involved in both the regulation of calcium uptake from the environment (Guerreiro et al, 2001) and regulates calcium resorption from scales (Rotllant et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Abbink

Kulczykowska

Kalamarz

et al. 2008

General and Comparative Endocrinology

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Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5& and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Inter actions/relations between melatonin and calcium balance and the hypercalcemic endocrines PTHrP and calcitriol were assessed. Restrict ing calcium availability in both water and diet had no effect on plasma melatonin, but when calcium was low in the water or absent from food, increased and decreased plasma melatonin was observed, respectively. Fish on a vitamin D-deficient diet (D-fish) showed decreased plasma calcitriol levels and remained normocalcemic. Decreased brain melatonin was found at all sampling times (10-22 weeks) in the D -fish compared to the controls. A positive correlation between plasma Ca2+ and plasma melatonin was found (R2 = 0.19; N = 41; P < 0.01) and brain melatonin was negatively correlated with plasma PTHrP (R2 = 0.78; N = 4; P < 0.05). The posi tive correlation between plasma levels of melatonin and Ca2+ provides evidence that melatonin synthesis is influenced by plasma Ca2+. The decreased melatonin production in the D -fish points to direct or indirect involvement of calcitriol in melatonin synthesis by the pineal organ in teleosts. The hypercalcemic factors PTHrP and calcitriol appeared to be negatively correlated with melatonin and this substantiates an involvement of melatonin in modulating the endocrine response to cope with hypocalcemia. It further points to the importance of Ca2+ in melatonin physiology.

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“…Pthrp's role in bony fish has been studied, (11,32,33) and it may be a hypercalcemic factor, particularly in the gills. (34) The role of Pthrp in elephant shark remains to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii)

Liu¹,

Ibrahim²,

Tay³

et al. 2010

Journal of Bone and Mineral Research

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The development of bone was a major step in the evolution of vertebrates. A bony skeleton provided structural support and a calcium reservoir essential for the movement from an aquatic to a terrestrial environment. Cartilaginous fishes are the oldest living group of jawed vertebrates. In this study we have identified three members of the parathyroid hormone (Pth) gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii). The three genes include two Pth genes, designated as Pth1 and Pth2, and a Pthrp gene. Phylogenetic analysis suggested that elephant shark Pth2 is an ancient gene whose orthologue is lost in bony vertebrates. The Pth1 and Pth2 genes have the same structure as the Pth gene in bony vertebrates, whereas the structure of the Pthrp gene is more complex in tetrapods compared with elephant shark. The three elephant shark genes showed distinct patterns of expression, with Pth2 being expressed only in the brain and spleen. This contrasts with localization of the corresponding proteins, which showed considerable overlap in their distribution. There were conserved sites of localization for Pthrp between elephant shark and mammals, including tissues such as kidney, skin, skeletal and cardiac muscle, pancreas, and cartilage. The elephant shark Pth1(1-34) and Pthrp(1-34) peptides were able to stimulate cAMP accumulation in mammalian UMR106.01 cells. However, Pth2(1-34) peptide did not show such PTH-like biologic activity. The presence of Pth and Pthrp genes in the elephant shark indicates that these genes played fundamental roles before their recruitment to bone development in bony jawed vertebrates. ß

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PTHrP regulation and calcium balance in sea bream (Sparus auratusL.) under calcium constraint

Cited by 25 publications

References 36 publications

Parathyroid hormone-related protein in teleost fish

Parathyroid hormone-related protein in teleost fish

Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Parathyroid hormone gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii)

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