Ptf1a triggers GABAergic neuronal cell fates in the retina

Dullin, Jean-Philippe; Locker, Morgane; Robach, Mélodie; Henningfeld, Kristine A.; Parain, Karine; Afelik, Solomon; Pieler, Tomas; Perron, Muriel

doi:10.1186/1471-213x-7-110

Cited by 62 publications

(70 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1B). The requirement for Ptf1a in the generation of many of these cell types has been demonstrated in mice and humans mutant for the gene (Dullin et al, 2007;Fujitani et al, 2006;Glasgow et al, 2005;Hoshino et al, 2005;Kawaguchi et al, 2002;Nakhai et al, 2007;Pascual et al, 2007;Sellick et al, 2004;Yamada et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Neurog2 is a direct downstream target of the Ptf1a-Rbpj transcription complex in dorsal spinal cord

et al. 2009

View full text Add to dashboard Cite

1, †PTF1-J is a trimeric transcription factor complex essential for generating the correct balance of GABAergic and glutamatergic interneurons in multiple regions of the nervous system, including the dorsal horn of the spinal cord and the cerebellum. Although the components of PTF1-J have been identified as the basic helix-loop-helix (bHLH) factor Ptf1a, its heterodimeric E-protein partner, and Rbpj, no neural targets are known for this transcription factor complex. Here we identify the neuronal differentiation gene Neurog2 (Ngn2, Math4A, neurogenin 2) as a direct target of PTF1-J. A Neurog2 dorsal neural tube enhancer localized 3Ј of the Neurog2 coding sequence was identified that requires a PTF1-J binding site for dorsal activity in mouse and chick neural tube. Gain and loss of Ptf1a function in vivo demonstrate its role in Neurog2 enhancer activity. Furthermore, chromatin immunoprecipitation from neural tube tissue demonstrates that Ptf1a is bound to the Neurog2 enhancer. Thus, Neurog2 expression is directly regulated by the PTF1-J complex, identifying Neurog2 as the first neural target of Ptf1a and revealing a bHLH transcription factor cascade functioning in the specification of GABAergic neurons in the dorsal spinal cord and cerebellum.

show abstract

Section: Introductionmentioning

confidence: 99%

Neurog2 is a direct downstream target of the Ptf1a-Rbpj transcription complex in dorsal spinal cord

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Atoh7 is a bHLH transcription factor required for the genesis of RGCs (Kanekar et al, 1997;Brown et al, 2001;Kay et al, 2001;Wang et al, 2001;Poggi et al, 2005). Ptf1a, which is also a bHLH transcription factor, is necessary and sufficient for the generation of retinal inhibitory neurons, the ACs and HCs (Fujitani et al, 2006;Dullin et al, 2007;Nakhai et al, 2007;Jusuf et al, 2011). Crx is a homeodomain transcription factor with multiple functions during retinal development, which is expressed in BCs and PRs in zebrafish (Liu et al, 2001;Shen and Raymond, 2004).…”

Section: The Transgenesmentioning

confidence: 99%

Spectrum of Fates: a new approach to the study of the developing zebrafish retina

Almeida¹,

Boije²,

Chow³

et al. 2014

Development

View full text Add to dashboard Cite

The ability to image cells live and in situ as they proliferate and differentiate has proved to be an invaluable asset to biologists investigating developmental processes. Here, we describe a Spectrum of Fates approach that allows the identification of all the major neuronal subtypes in the zebrafish retina simultaneously. Spectrum of Fates is based on the combinatorial expression of differently coloured fluorescent proteins driven by the promoters of transcription factors that are expressed in overlapping subsets of retinal neurons. Here, we show how a Spectrum of Fates approach can be used to assess various aspects of neural development, such as developmental waves of differentiation, neuropil development, lineage tracing and hierarchies of fates in the developing zebrafish retina.

show abstract

“…Similar to other sites, in the cerebellum, projection neurons are generated first, and local interneurons follow. In addition, transcription factors relevant for the specification of cerebellar GABAergic types, such as Ptf1-a or Pax-2, also participate to the same processes in other CNS regions [7,8,32,[57][58][59][60]. Nevertheless, a common theme in the genesis of interneurons along the neuraxis is that different phenotypes are generated from distinct subsets of fate-committed progenitors.…”

Section: Concluding Remarks: a Peculiar Neurogenic Strategymentioning

confidence: 99%

Development of Cerebellar GABAergic Interneurons: Origin and Shaping of the “Minibrain” Local Connections

2008

View full text Add to dashboard Cite

The cerebellar circuits comprise a limited number of neuronal phenotypes embedded in a defined cytoarchitecture and generated according to specific spatio-temporal patterns. The local GABAergic network is composed of several interneuron phenotypes that play essential roles in information processing by modulating the activity of cerebellar cortical inputs and outputs. A major issue in the study of cerebellar development is to understand the mechanisms that underlie the generation of different interneuron classes and regulate their placement in the cerebellar architecture and integration in the cortico-nuclear network. Recent findings indicate that the variety of cerebellar interneurons derives from a single population of multipotent progenitors whose fate choices are determined by instructive environmental information. Such a strategy, which is unique for the cerebellum along the neuraxis, allows great flexibility in the control of the quality and quantity of GABAergic interneurons that are produced, thus facilitating the adaptive shaping of the cerebellar network to specific functional demands.

show abstract

Ptf1a triggers GABAergic neuronal cell fates in the retina

Cited by 62 publications

References 64 publications

Neurog2 is a direct downstream target of the Ptf1a-Rbpj transcription complex in dorsal spinal cord

Neurog2 is a direct downstream target of the Ptf1a-Rbpj transcription complex in dorsal spinal cord

Spectrum of Fates: a new approach to the study of the developing zebrafish retina

Development of Cerebellar GABAergic Interneurons: Origin and Shaping of the “Minibrain” Local Connections

Contact Info

Product

Resources

About