Pteropine Orthoreovirus, PRV7S (Sikamat Virus) Demonstrates Oncolysis in Nasopharyngeal Carcinoma Cell Lines

Loh, Alson; Shaibie, Nur Adania; Chan, Po Qhuan; Leong, Pooi Pooi; Segeran, Sharrada; Tan, Boon Keat; Wong, Siew Tung; Koh, Rhun Yian; Chye, Soi Moi; Voon, Kenny

doi:10.31083/j.fbl2704138

Cited by 4 publications

(4 citation statements)

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“…Real‐time PCR was performed using SensiFAST SYBR kit (Bioline). Modified primers sequences of PRVSig1F2 5′‐TGCTGATTGGAAYGCTGACT‐3′ and PRVSig1R2 5′‐CKGAAAAGGYTTGAKACGCC‐3′ were used for quantification of PRV by targeting the Sigma 1/A gene 8,19 . The qPCR protocol started with 95°C for 3 min followed by 40 cycles of 95°C for 10 s then 57°C for 30 s and the last step was 72°C for 50 s, with data collection and real‐time analysis by iQ5 real‐time PCR detection system (Bio‐Rad Laboratories).…”

Section: Methodsmentioning

confidence: 99%

“…Cells were observed for cytopathic effect (CPE) for 5 days postinfection and the titers were expressed as TCID 50 /ml. Multiplicity of infection (MOI) was calculated based on the TCID 50 as previously described 19 . For virus kinetic study, OKF‐6, NP69 and AC16 cells were infected with 12MYKLU1034, PRV3M or PRV4K, respectively, at a MOI of 0.1.…”

Section: Methodsmentioning

confidence: 99%

“…Multiplicity of infection (MOI) was calculated based on the TCID 50 as previously described. 19 For virus kinetic study, OKF-6, NP69 and AC16 cells were infected with 12MYK-LU1034, PRV3M or PRV4K, respectively, at a MOI of 0.1. After adsorption for 1 h, the inocula were removed, and the cells were incubated with the appropriate complete media at 37°C and 5% CO 2 , except for AC16 cells which were cultured in DMEM/F12 with 2% FBS with 1% penicillin/streptomycin.…”

Section: Virus Titer and Virus Kinetics Studymentioning

confidence: 99%

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Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia

Tee

Chan

Loh

et al. 2023

Journal of Medical Virology

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Pteropine orthoreovirus (PRV), an emerging bat‐borne virus, has been linked to cases of acute respiratory infections (ARI) in humans. The prevalence, epidemiology and genomic diversity of PRV among ARI of unknown origin were studied. Among 632 urban outpatients tested negative for all known respiratory viruses, 2.2% were PRV‐positive. Patients mainly presented with moderate to severe forms of cough, sore throat and muscle ache, but rarely with fever. Phylogenetic analysis revealed that over 90% of patients infected with the Melaka virus (MelV)‐like PRV, while one patient infected with the Pulau virus previously found only in fruit bats. Human oral keratinocytes and nasopharyngeal epithelial cells were susceptible to clinical isolates of PRV, including the newly isolated MelV‐like 12MYKLU1034. Whole genome sequence of 12MYKLU1034 using Nanopore technique revealed a novel reassortant strain. Evolutionary analysis of the global PRV strains suggests the continuous evolution of PRV through genetic reassortment among PRV strains circulating in human, bats and non‐human primate hosts, creating a spectrum of reassortant lineages with complex evolutionary characteristics. In summary, the role of PRV as a common etiologic agent of ARI is evident. Continuous monitoring of PRV prevalence, pathogenicity and diversity among human and animal hosts is important to trace the emergence of novel reassortants.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Virus Titer and Virus Kinetics Studymentioning

confidence: 99%

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Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia

Tee

Chan

Loh

et al. 2023

Journal of Medical Virology

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“…Oncolytic viruses have been genetically engineered to express cytokines, costimulatory molecules, immune checkpoint inhibitors, or bispecific T-cell engager (BiTE) molecules to boost anti-tumour immune response and suppress tumour tolerance. For instance, an oncolytic adenovirus MG1 encoding HPV oncogenes E6 and E7 demonstrated tumour-specific responses in preclinical studies and is currently undergoing a phase I clinical trial involving patients with HPV-associated malignancies ( Wang et al, 2011 ; Loh et al, 2022 ; Tan et al, 2022 ). Thus, oncolytic viruses represent attractive targets for the development of therapeutic tools against EBV-associated NPC.…”

Section: Ongoing Clinical and Preclinical Studies On Therapeutic Targ...mentioning

confidence: 99%

The role of Epstein–Barr virus in nasopharyngeal carcinoma

Siak

Leong³

et al. 2023

Front. Microbiol.

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Nasopharyngeal carcinoma (NPC) is a metastasis-prone malignancy closely associated with the Epstein–Barr virus (EBV). Despite ubiquitous infection of EBV worldwide, NPC incidences displayed predominance in certain ethnic groups and endemic regions. The majority of NPC patients are diagnosed with advanced-stage disease, as a result of anatomical isolation and non-specific clinical manifestation. Over the decades, researchers have gained insights into the molecular mechanisms underlying NPC pathogenesis as a result of the interplay of EBV infection with several environmental and genetic factors. EBV-associated biomarkers were also used for mass population screening for the early detection of NPC. EBV and its encoded products also serve as potential targets for the development of therapeutic strategies and tumour-specific drug delivery. This review will discuss the pathogenic role of EBV in NPC and efforts in exploiting the potential of EBV-associated molecules as biomarkers and therapeutic targets. The current knowledge on the role of EBV and its associated products in NPC tumorigenesis, development and progression will offer a new outlook and potential intervention strategy against this EBV-associated malignancy.

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Human cytokeratin 1 plays a role in the interaction of Pteropine orthoreovirus with Hek293 cells but not HeLa cells

Siew,

Tan,

Iswara

et al. 2024

Microbes and Infection

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Pteropine Orthoreovirus, PRV7S (Sikamat Virus) Demonstrates Oncolysis in Nasopharyngeal Carcinoma Cell Lines

Cited by 4 publications

References 0 publications

Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia

Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia

The role of Epstein–Barr virus in nasopharyngeal carcinoma

Human cytokeratin 1 plays a role in the interaction of Pteropine orthoreovirus with Hek293 cells but not HeLa cells

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