Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus

Bennett, Andrew J.; Goldberg, Tony L.

doi:10.3390/v12070740

Cited by 9 publications

(12 citation statements)

References 47 publications

(81 reference statements)

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“…It is also worthy to note that two African PRV strains do not appear as a different group from the Asian PRV clades. As has been PLOS NEGLECTED TROPICAL DISEASES previously suggested, it is possible that PRV in Africa did not co-diverge with African fruit bats from their ancestral Asian lineages in the Miocene, but rather spread between African and Asian bats more recently [19]. Although our results support this suggestion, further epidemiological studies in Africa and Asia are required to understand the origin of PRV.…”

Section: Plos Neglected Tropical Diseasessupporting

confidence: 75%

“…To identify PRV strains genetically related to Nachunsulwe-57, BLAST analyses were performed using the sequences of each viral segment (Table 3). Nine of 10 segments (L1, L2, M1-M3, and S1-S4) of Nachunsulwe-57 were closely related to those of Kasama strain, which was detected in an Angolan soft-furred fruit bat (Lissonycteris angolensis) in Uganda [19]. The…”

Section: Genetic Characterization Of Nachunsulwe-57mentioning

confidence: 89%

“…PRV in a fruit bat in Uganda [19], there is essentially no information about the prevalence of PRV infection in Africa. This is the first report of the PRV surveillance and isolation in fruit bats in Africa.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

“…PRVs were also isolated from monkey feces collected in Thailand, raising the hypothesis that other mammals, apart from bats, may also play a role in PRV transmission to humans [18]. Recently, a partial PRV genome was detected in an Angolan soft-furred fruit bat (Lissonycteris angolensis) in Uganda via a metagenomic approach, suggesting the new geographic distribution of PRV outside of Asia and Australia [19]. However, the prevalence and pathogenicity of PRVs in Africa are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia

Harima¹,

Sasaki

Orba

et al. 2021

PLoS Negl Trop Dis

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Background Pteropine orthoreovirus (PRV) is an emerging bat-borne zoonotic virus that causes severe respiratory illness in humans. Although PRVs have been identified in fruit bats and humans in Australia and Asia, little is known about the prevalence of PRV infection in Africa. Therefore, this study performed an PRV surveillance in fruit bats in Zambia. Methods Egyptian fruit bats (Rousettus aegyptiacus, n = 47) and straw-colored fruit bats (Eidolon helvum, n = 33) captured in Zambia in 2017–2018 were screened for PRV infection using RT-PCR and serum neutralization tests. The complete genome sequence of an isolated PRV strain was determined by next generation sequencing and subjected to BLAST and phylogenetic analyses. Replication capacity and pathogenicity of the strain were investigated using Vero E6 cell cultures and BALB/c mice, respectively. Results An PRV strain, tentatively named Nachunsulwe-57, was isolated from one Egyptian fruit bat. Serological assays demonstrated that 98% of sera (69/70) collected from Egyptian fruit bats (n = 37) and straw-colored fruit bats (n = 33) had neutralizing antibodies against PRV. Genetic analyses revealed that all 10 genome segments of Nachunsulwe-57 were closely related to a bat-derived Kasama strain found in Uganda. Nachunsulwe-57 showed less efficiency in viral growth and lower pathogenicity in mice than another PRV strain, Miyazaki-Bali/2007, isolated from a patient. Conclusions A high proportion of Egyptian fruit bats and straw-colored fruit bats were found to be seropositive to PRV in Zambia. Importantly, a new PRV strain (Nachunsulwe-57) was isolated from an Egyptian fruit bat in Zambia, which had relatively weak pathogenicity in mice. Taken together, our findings provide new epidemiological insights about PRV infection in bats and indicate the first isolation of an PRV strain that may have low pathogenicity to humans.

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Section: Plos Neglected Tropical Diseasessupporting

confidence: 75%

Section: Genetic Characterization Of Nachunsulwe-57mentioning

confidence: 89%

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia

Harima¹,

Sasaki

Orba

et al. 2021

PLoS Negl Trop Dis

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“…In Uganda, we trapped bats in Kamwenge District at Ngogo research station within Kibale National Park (0 • 29 7.97 N, 30 • 23 21.91 E at 1550 m elevation; semideciduous montane forest) in July 2017, as previously described [14,[17][18][19] (Figure 1). Briefly, we set mist nets at a large hollow tree roost and captured bats as they exited at dusk.…”

Section: Study Sites and Samplingmentioning

confidence: 99%

Bat Flies of the Family Streblidae (Diptera: Hippoboscoidea) Host Relatives of Medically and Agriculturally Important “Bat-Associated” Viruses

Ramírez‐Martínez

Bennett

Dunn

et al. 2021

Viruses

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Bat flies (Hippoboscoidea: Nycteribiidae and Streblidae) are obligate hematophagous ectoparasites of bats. We collected streblid bat flies from the New World (México) and the Old World (Uganda), and used metagenomics to identify their viruses. In México, we found méjal virus (Rhabdoviridae; Vesiculovirus), Amate virus (Reoviridae: Orbivirus), and two unclassified viruses of invertebrates. Méjal virus is related to emerging zoonotic encephalitis viruses and to the agriculturally important vesicular stomatitis viruses (VSV). Amate virus and its sister taxon from a bat are most closely related to mosquito- and tick-borne orbiviruses, suggesting a previously unrecognized orbivirus transmission cycle involving bats and bat flies. In Uganda, we found mamucuso virus (Peribunyaviridae: Orthobunyavirus) and two unclassified viruses (a rhabdovirus and an invertebrate virus). Mamucuso virus is related to encephalitic viruses of mammals and to viruses from nycteribiid bat flies and louse flies, suggesting a previously unrecognized orthobunyavirus transmission cycle involving hippoboscoid insects. Bat fly virus transmission may be neither strictly vector-borne nor strictly vertical, with opportunistic feeding by bat flies occasionally leading to zoonotic transmission. Many “bat-associated” viruses, which are ecologically and epidemiologically associated with bats but rarely or never found in bats themselves, may actually be viruses of bat flies or other bat ectoparasites.

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Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia

Tee

Chan

Loh

et al. 2023

Journal of Medical Virology

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Pteropine orthoreovirus (PRV), an emerging bat‐borne virus, has been linked to cases of acute respiratory infections (ARI) in humans. The prevalence, epidemiology and genomic diversity of PRV among ARI of unknown origin were studied. Among 632 urban outpatients tested negative for all known respiratory viruses, 2.2% were PRV‐positive. Patients mainly presented with moderate to severe forms of cough, sore throat and muscle ache, but rarely with fever. Phylogenetic analysis revealed that over 90% of patients infected with the Melaka virus (MelV)‐like PRV, while one patient infected with the Pulau virus previously found only in fruit bats. Human oral keratinocytes and nasopharyngeal epithelial cells were susceptible to clinical isolates of PRV, including the newly isolated MelV‐like 12MYKLU1034. Whole genome sequence of 12MYKLU1034 using Nanopore technique revealed a novel reassortant strain. Evolutionary analysis of the global PRV strains suggests the continuous evolution of PRV through genetic reassortment among PRV strains circulating in human, bats and non‐human primate hosts, creating a spectrum of reassortant lineages with complex evolutionary characteristics. In summary, the role of PRV as a common etiologic agent of ARI is evident. Continuous monitoring of PRV prevalence, pathogenicity and diversity among human and animal hosts is important to trace the emergence of novel reassortants.

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Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus

Cited by 9 publications

References 47 publications

Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia

Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia

Bat Flies of the Family Streblidae (Diptera: Hippoboscoidea) Host Relatives of Medically and Agriculturally Important “Bat-Associated” Viruses

Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia

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