pT3.2I, the Smallest Plasmid of Thiobacillus T3.2

Aparicio, Tomás; Lorenzo, Paloma; Perera, Julián

doi:10.1006/plas.2000.1461

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“…PTF-FC2 was related to a broad host range plasmid RSF1010 [8] and pTAV1 was related to some plasmids from Agrobacterium and Rhizobium species [9]. Recently described pTiK12 [10] and pT3.21 [11] were related to ColE2 and ColE3 [12], respectively.…”

Section: Introductionmentioning

confidence: 99%

A broad host range plasmid vector that does not encode replication proteins

Kalyaeva

2002

FEMS Microbiology Letters

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The 640-bp minimal replication region derived from a plasmid DNA preparation from an Acidothiobacillus ferrooxidans strain capable of autonomous replication in a range of Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Acinetobacter calcoaceticus and Alcaligenes faecalis) was identified. This DNA fragment (named TFK replicon) does not encode Rep proteins and appears to be unrelated to other known replicons. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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