Pt(II) complex of Schiff base derived from L-phenylalanine and furfuraldehyde in the presence of 8-hydroxyquinoline: Structural analysis, composition of complex and biological activity

Abstract

In the present study, we synthesized a Schiff base (L 1) derived from L-phenylalanine with furfuraldehyde and its Pt(II) complex ([Pt(L 1)(L 2)]: [Pt(N-(furfuralidene)phenylalanine)(8hydroxyquinoline)]) in the presence of 8-hydroxyquinoline (L 2). The chemical structures of the synthesized Schiff base and Pt(II) complex were characterized using ESI-MS, UV-Visible, FT-IR, 1 H NMR, 13 C NMR, and powder-XRD spectroscopy. The surface morphologies and elemental composition were determined by SEM and EDX. Thermal analysis was performed with TG-DTA. Mole ratio of Meyer and Job methods were used for the composition of complexes. According to these methods, the ratio [Pt 2+ ] : [L 1 + L 2 ] was found to be 1 : 1. In addition, the antimicrobial activities of L 1 and the complex showed that the compounds have significant antibacterial and antifungal properties. They also show important cytotoxic effects against the growth of mouse embryo fibroblasts (MEF) and human prostate adenocarcinoma (Du145) cancer cells. L 1 , L 2 , and the Pt(II) complex also displayed effective antioxidant activity.

Synthesis of the Bacteriostatic Poly(l-Lactide) by Using Zinc (II)[(acac)(L)H2O] (L = Aminoacid-Based Chelate Ligands) as an Effective ROP Initiator

“…Furthermore, the shift in signals and splitting of protons of the aryl groups were observed, especially in the spectrum of Zn[(acac)(Lphe)] (Figure4a, in the range of 8.7-7.5 ppm). A similar phenomenon was already observed in the spectra of the Pt(II) complexes of the Schiff base derived from L-phenylalanine and furfuraldehyde[31]. The signals assigned to water protons were much more intensive than those of water absorbed in DMSO d6; this most probably indicated that the synthesized complexes also contained a water molecule, similar to that for Zn[(acac)2 H2O].…”

Synthesis and Biological Activity of a New Pt(II) Complex Involving 4-bromo-2,6-bis-hydroxymethyl-phenol and Nicotinamide

“…The antimicrobial activities of the BBHMP and platinum complex were tested against gram(-) Escherichia coli (ATCC® 25922™), Salmonella typhimurium (ATCC® 14028™), Listeria monocytogenes (ATCC® 19115™) gram (+) Staphylococcus aureus (ATCC® 25923™), Bacillus cereus (ATCC® 11778™), and fungal Candida albicans (ATCC® 1023™). The micro broth dilution method and Clinical and Laboratory Standards Institute (CLSI) [21][22][23][24][25] procedures were used to nd the minimal inhibition concentration values (MIC). To measure the area for zone of inhibition, Ampicillin for bacterial strains and Amphotericin B for fungal strains were chosen as antibiotic (positive control).…”

“…The cytotoxicity was determined by MTT assay [25][26][27][28]. The MTT method is a widely used cell viability test for cytotoxicity and can be used with all cancer cell types because it metabolizes all living cells.…”

“…cDNA synthesis was realized with the thermal cycler Applied Biosystems® Veriti® (Thermo Scienti c, USA) at the three steps which were Step 1: 25 °C, 10 min; Step 2: 37 °C, 120 min; Step 3: 85 °C, 5 min, respectively. The synthesized cDNA was kept at -20 °C for later analysis steps [25,28,[30][31][32][33]. Expression levels of the mitochondrial apoptosis genes in both cancer cells of vehicle treated control and IC 50 doses treated with BBHMP or Pt(II) complex for 24 hours were determined with quantitative real-time-polymerase chain reaction (qRT-PCR) by using the PowerSYBR® Select Master Mix (Life Technologies, USA) by an ABI 7500 Real-Time PCR system (Thermo Scienti c, USA).…”

“…Antioxidants are free radical scavengers and the DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay is a simple and rapid method widely used to determine the free radical scavenging capacity of different antioxidants [25,[34][35][36]. The radical in DPPH gives a purple color with maximum absorption at 517 nm.…”