Psychrotrophic bacteria

Brasca, Milena; Decimo, Marilù; Morandi, Stefano; Machado, Solimar Gonçalves; Baglinière, François; Vanetti, Maria Cristina Dantas

doi:10.1002/9781119115007.ch3

Cited by 7 publications

(4 citation statements)

References 156 publications

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“…This latter mechanism improves their adaptability and spreading, making their eradication very challenging and enhancing their drug resistance [47]. Because of these characteristics, the occurrence of some psychrotrophic pseudomonads (i.e., P. fluorescens , P. fragi , P. lundensis , P. gessardii , and P. taetrolens ) in refrigerated fresh dairy products, as well as in potable water used as governing liquid, has been gaining increasing interest [20,48,49].…”

Section: Pseudomonas Spp Genus and Species Occurrence In Dairy Prmentioning

confidence: 99%

“…MLST has proved to be a powerful tool for characterizing bacteria based on allelic variations of housekeeping genes. Interestingly, some of these genes (i.e., caseinolytic metallo-protease gene aprX [61]) and targeted MLST phylogenetic schemes (based on an housekeeping sequences uploaded to the Pseudomonas MLST databases and ) have been very effective at discriminating and tracing psychrotrophic pseudomonads involved in proteolysis of spoilage cheeses [62,63] and fresh cheese discoloration [64]; in particular, the blue discoloration of different fresh cheeses (such as high moisture mozzarella cheese, Latin style low acid fresh cheese, and ricotta cheese), recognized as a worldwide problem, was attributed to a “blue branch” of the P. fluorescens phylogenetic tree [39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65].…”

Section: Pseudomonas Spp Genus and Species Occurrence In Dairy Prmentioning

confidence: 99%

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Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Quintieri

Fanelli

Caputo

2019

Foods

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Microbial multidrug resistance (MDR) is a growing threat to public health mostly because it makes the fight against microorganisms that cause lethal infections ever less effective. Thus, the surveillance on MDR microorganisms has recently been strengthened, taking into account the control of antibiotic abuse as well as the mechanisms underlying the transfer of antibiotic genes (ARGs) among microbiota naturally occurring in the environment. Indeed, ARGs are not only confined to pathogenic bacteria, whose diffusion in the clinical field has aroused serious concerns, but are widespread in saprophytic bacterial communities such as those dominating the food industry. In particular, fresh dairy products can be considered a reservoir of Pseudomonas spp. resistome, potentially transmittable to consumers. Milk and fresh dairy cheeses products represent one of a few “hubs” where commensal or opportunistic pseudomonads frequently cohabit together with food microbiota and hazard pathogens even across their manufacturing processes. Pseudomonas spp., widely studied for food spoilage effects, are instead underestimated for their possible impact on human health. Recent evidences have highlighted that non-pathogenic pseudomonads strains (P. fluorescens, P. putida) are associated with some human diseases, but are still poorly considered in comparison to the pathogen P. aeruginosa. In addition, the presence of ARGs, that can be acquired and transmitted by horizontal genetic transfer, further increases their risk and the need to be deeper investigated. Therefore, this review, starting from the general aspects related to the physiological traits of these spoilage microorganisms from fresh dairy products, aims to shed light on the resistome of cheese-related pseudomonads and their genomic background, current methods and advances in the prediction tools for MDR detection based on genomic sequences, possible implications for human health, and the affordable strategies to counteract MDR spread.

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Section: Pseudomonas Spp Genus and Species Occurrence In Dairy Prmentioning

confidence: 99%

Section: Pseudomonas Spp Genus and Species Occurrence In Dairy Prmentioning

confidence: 99%

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Quintieri

Fanelli

Caputo

2019

Foods

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“…As for AprX, the primary structure of Ser1 shows the presence of the consensus motives HEXXHXUGUXH and GGXGXDXUX for the binding of Zn 2+ and Ca 2+ , respectively. Moreover, Ser1 proteases contain the ABC exporter motif (DXXX), indicating they are extracellular enzymes [ 26 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

Proteolytic Traits of Psychrotrophic Bacteria Potentially Causative of Sterilized Milk Instability: Genotypic, Phenotypic and Peptidomic Insight

Morandi

Pica

Masotti

et al. 2021

Foods

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The proteolytic traits of the psychrotrophic strains Pseudomonas poae LP5, Pseudomonas fluorescens LPF3, Chryseobacterium joostei LPR1, Pseudomonas fulva PS1, Citrobacter freundii PS37, Hafnia alvei PS46, and Serratia marcescens PS92 were initially investigated by phenotypic and genotypic approaches. Six strains elicited extracellular proteolytic activity, and five expressed the thermostable AprX or (likely) Ser1 enzymes. Then, the strains were inoculated (104 CFU/mL) in microfiltered pasteurized milk and kept at 4 °C for five days. All of the strains reached 108 CFU/mL at the end of storage and five produced thermostable extracellular proteolytic enzymes. The freshly inoculated samples and the corresponding samples at 108 CFU/mL were batch-sterilized (131 °C, 30 s) and kept at 45 °C up to 100 days. The former samples did not gel until the end of incubation, whereas the latter, containing P. poae, P. fluorescens, C. joostei, C. freundii, and S. marcescens, gelled within a few days of incubation. The thermostable proteolytic activity of strains affected the peptidomic profile, and specific proteolyzed zones of β-CN were recognized in the gelled samples. Overall, the results confirm some proteolytic traits of psychrotrophic Pseudomonas spp. strains and provide additional insights on the proteolytic activity of psychrotrophic bacteria potentially responsible for sterilized milk destabilization.

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“…As a consequence of these features, the existence of Pseudomonas spp. such as P. lundensis , P. fragi , P. fluorescens , P. gessardii , and P. taetrolens in hilled fresh food products has been developing great attention (Baruzzi et al 2012 ; Guidone et al 2016 ; Brasca et al 2018 ; Quintieri et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas species prevalence, protein analysis, and antibiotic resistance: an evolving public health challenge

et al. 2022

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Psychrotrophic Pseudomonas is one of the significant microbes that lead to putrefaction in chilled meat. One of the biggest problems in the detection of Pseudomonas is that several species are seemingly identical. Currently, antibiotic resistance is one of the most significant challenges facing the world's health and food security. Therefore, this study was designed to apply an accurate technique for eliminating the identification discrepancy of Pseudomonas species and to study their resistance against various antimicrobials. A total of 320 chicken meat specimens were cultivated, and the isolated bacteria’ were phenotypically recognized. Protein analysis was carried out for cultured isolates via Microflex LT. The resistance of Pseudomonas isolates was recorded through Vitek® 2 AST-GN83 cards. Overall, 69 samples were identified as Pseudomonas spp. and included 18 Pseudomonas lundensis (P. lundensis), 16 Pseudomonas fragi (P. fragi), 13 Pseudomonas oryzihabitans (P. oryzihabitans), 10 Pseudomonas stutzeri (P. stutzeri), 5 Pseudomonas fluorescens (P. fluorescens), 4 Pseudomonas putida (P. putida), and 3 Pseudomonas aeruginosa (P. aeruginosa) isolates. Microflex LT identified all Pseudomonas isolates (100%) correctly with a score value ≥ 2.00. PCA positively discriminated the identified isolates into various groups. The antimicrobial resistance levels against Pseudomonas isolates were 81.16% for nitrofurantoin, 71% for ampicillin and ampicillin/sulbactam, 65.22% for cefuroxime and ceftriaxone, 55% for aztreonam, and 49.28% for ciprofloxacin. The susceptibilities were 100% for cefotaxime, 98.55% for ceftazidime, 94.20% for each piperacillin/tazobactam and cefepime, 91.3% for cefazolin. In conclusion, chicken meat was found to be contaminated with different Pseudomonas spp., with high incidence rates of P. lundensis. Microflex LT is a potent tool for distinguishing Pseudomonads at the species level.

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Psychrotrophic bacteria

Cited by 7 publications

References 156 publications

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Proteolytic Traits of Psychrotrophic Bacteria Potentially Causative of Sterilized Milk Instability: Genotypic, Phenotypic and Peptidomic Insight

Pseudomonas species prevalence, protein analysis, and antibiotic resistance: an evolving public health challenge

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