Psychrobacter sanguinis sp. nov., recovered from four clinical specimens over a 4-year period

Abstract

An analysis of 16S rRNA gene sequences from archived clinical reference specimens identified a novel species of the genus Psychrobacter, of which four strains have been independently isolated from human blood. On the basis of 16S rRNA gene sequence similarity, the closest relatives with validly published names were Psychrobacter arenosus R7 T (98.7 %), P. pulmonis CECT 5989 T (97.7 %), P. faecalis Iso-46 T (97.6 %) and P. lutiphocae IMMIB L-1110 T (97.2 %). Maximumlikelihood phylogenetic analysis of 16S rRNA gene sequences showed that the isolates belonged to the genus Psychrobacter and were members of a cluster associated with Psychrobacter sp. PRwf-1, isolated from a silk snapper fish. DNA-DNA relatedness and partial 23S rRNA gene sequences also supported the finding that the isolates belonged to a species distinct from its closest phylogenetic neighbours. The predominant cellular fatty acids were C 18 : 1 v9c, C 16 : 0 , summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH), summed feature 5 (C 18 : 2 v6,9c and/or anteiso-C 18 : 0 ) and C 18 : 0 . Biochemical and morphological analysis further supported the assignment of the four isolates to a novel species. The name Psychrobacter sanguinis sp. nov. is proposed. The type strain is 13983 T (5DSM 23635 T 5CCUG 59771 T ).

Psychrobacter sanguinis: an Unusual Bacterium for Nosocomial Meningitis

“…Whereas Psychrobacter spp. usually grow on marine agar, which contains 3.5% NaCl, our P. sanguinis isolate failed to grow on this specific medium, as described by Wirth et al (7). Moreover, our isolate was able to grow between the temperatures of 4°C and 37°C.…”

First Case ofPsychrobacter sanguinisBacteremia in a Korean Patient

“…We report a first case in which P. sanguinis was isolated from human blood in Korea. In the six previously reported cases, we didn't detailed review for first reported case and the other three cases due to a lack of information in the literature [11] and two cases with meningitis were described as a probable infection [13] or a definite infection [12]. These cases were confirmed as a single organism with P. sanguinis infection by using molecular methods, and were treated with antibiotics.…”

“…This represented the first report of P. sanguinis in humans, and the type strain was isolated from the blood of an 84-year-old male in 2012 [11]. In 2014, P. sanguinis was isolated from the CSF of a 64-year-old female patient with post-neurosurgical meningitis, and this strain was identified only by 16S rRNA analysis [12].…”

“…In 2012, P. sanguinis was first described as a novel species based on four clinical specimens, and the type strain was isolated from the blood of an 84-year-old male in New York [11].…”

“…Since the initial description of this microorganism, six cases of P. sanguinis infection have been reported in humans, specifically blood specimens and CSF [11][12][13].…”