Psychostomatology: The psychosomatic status and approaches for the management of patients with inflammatory oral mucosal diseases

Abiko, Yoshihiro; Paudel, Durga; Matsuoka, Hirofumi; Yamazaki, Yutaka; Koga, Chihiro; Kitagawa, Yoshimasa; Toyofuku, Akira

doi:10.1016/j.ajoms.2021.08.007

Cited by 2 publications

(3 citation statements)

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“…Reduced alpha diversity has been observed in patients with RAS and oral lichen planus [ 46 ]. Our findings may be consistent with the concept that psychological stress is an etiology of RAS and oral lichen planus [ 2 ]. A reduction in the alpha diversity of the oral microbiota was observed in patients with oral mucositis after radiotherapy [ 47 ] thus indicating that reduced alpha diversity due to psychological stress might be considered as a risk factor for oral mucositis.…”

Section: Discussionsupporting

confidence: 93%

“…The involvement of multiple factors makes it difficult to understand the underlying pathogenesis of these diseases. Psychological stress might be one of the important factors [ 2 , 3 ]; however, its role in the etiology of these diseases remains unclear. Alterations in the oral environment, including the saliva and the oral microbiota, caused by psychological stress could affect the etiologies of these diseases.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic and microbiota analyses of the oral cavity during psychological stress

et al. 2022

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Psychological stress is associated with various oral diseases such as aphthous stomatitis, oral lichen planus, taste disturbances and glossodynia. However, the underlying mechanism is still unknown. The aim of this study was to determine the effect of psychological stress on salivary proteins and the oral microbiota in a rat model of chronic restraint stress. Six-week-old Sprague Dawley rats were subjected to restraint stress for four hours daily for 1 month. The behavior, weights of the adrenal glands, and serum corticosterone levels were evaluated as stress markers. Proteomic analysis of the saliva was performed using two-dimensional gel electrophoresis followed by mass spectrometry and Western blotting. Analysis of the oral microbiota was performed via 16S rRNA next-generation sequencing. The low mean body weights, lower number of entries and time spent in the open arm of elevated plus maze, high adrenal gland/body weight ratios, and high serum corticosterone levels confirmed the high levels of stress in the stress group of rats compared to the controls. Thirty-three protein spots were found to be significantly altered between the two groups. After silver staining, seven visible spots were subjected for mass spectrometry, and the expression levels of the two most significantly altered proteins, BPI fold containing family A member 2 and von Ebner’s gland protein, were confirmed by Western blotting. 16S rRNA sequencing analysis revealed a significant reduction in alpha diversity in the stress group compared to the controls. The abundances of oral bacteria, such as Facklamia and Corynebacterium, were significantly altered between the two groups. Additionally, analysis with PICRUSt2 software predicted 37 different functional pathways to be altered between the groups. In conclusion, the present study identified altered salivary proteins and oral microbiota due to psychological stress. These findings might aid in understanding the pathogenesis of stress-related oral diseases.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Proteomic and microbiota analyses of the oral cavity during psychological stress

et al. 2022

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“…Psychological stress has been implicated in the pathogenesis of various oral diseases such as xerostomia, burning mouth syndrome, and aphthous stomatitis 1–3 . Changes in the local environment of the oral cavity often accompany these diseases.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the submandibular gland under psychological stress condition

Paudel,

Uehara,

Giri

et al. 2024

J Oral Pathology Medicine

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BackgroundPsychological stress is associated with changes in salivary flow and composition. However, studies to show the effect of psychological stress on the transcriptome of the salivary gland are limited. This study aims to perform a transcriptomic analysis of the submandibular gland under psychological stress using a chronic restraint stress model of rats.MethodsSprague‐Dawley rats were divided into stress groups and control groups. Psychological stress was induced in the stress group rats by enclosing them in a plastic tube for 4 h daily over 6 weeks. RNA sequencing was performed on RNA extracted from the submandibular gland. The differentially expressed genes were identified, and the genes of interest were further validated using qRT‐PCR, immunofluorescence, and western blot.ResultsA comparison between control and stress groups showed 45 differentially expressed genes. The top five altered genes in RNA sequencing data showed similar gene expression in qRT‐PCR validation. The most downregulated gene in the stress group, FosB, was a gene of interest and was further validated for its protein‐level expression using immunofluorescence and western blot. The genesets for gene ontology cellular component, molecular function, and KEGG showed that pathways related to ribosome biosynthesis and function were downregulated in the stress group compared to the control.ConclusionPsychological stress showed transcriptomic alteration in the submandibular gland. The findings may be important in understanding stress‐related oral diseases.

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Psychostomatology: The psychosomatic status and approaches for the management of patients with inflammatory oral mucosal diseases

Cited by 2 publications

References 90 publications

Proteomic and microbiota analyses of the oral cavity during psychological stress

Proteomic and microbiota analyses of the oral cavity during psychological stress

Transcriptomic analysis of the submandibular gland under psychological stress condition

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