Reliable and valid measurements of anxiety, chronic worry, depressive, and obsessive-compulsive symptoms is essential when seeking to draw cross-cultural and gender comparisons. Accordingly, the current study determined the psychometric properties of five widely used symptom measures in the United States (U.S.) and Singapore (SG) and across gender: Beck Anxiety Inventory (BAI), Beck Depression Inventory-II (BDI-II), Obsessive Beliefs Questionnaire (OBQ), Obsessive-Compulsive Inventory-Revised (OCI-R), and the Penn State Worry Questionnaire-Abbreviated (PSWQ-A). Participants comprised college students (U.S.: n = 292; SG: n = 144). Strict measurement invariance (equal factor loadings, item thresholds, residual variances) was observed for all measures across cultures and genders, except for the BAI, which met partial Level 1 strict cross-gender invariance. Overall, findings suggest a strong degree of cross-cultural and cross-gender construct compatibility, but also point to some notable exceptions, thereby providing ample material for theory-building and future research on transdiagnostic symptom and cognitive vulnerability measures across diverse groups.