Objective-Vulvodynia is a chronic pain syndrome of unknown etiology with scant data on frequency. This study assessed the prevalence of vulvodynia symptoms in a sample of U.S. women and compared health characteristics of symptomatic and asymptomatic women.Study Design-A phone survey contacted 2,127 U.S. households to identify 100 symptomatic women, who were matched on age and time-zone to 325 asymptomatic controls. Odds ratios and logistic regression were used to model associations between pain, medical conditions, and healthcare utilization variables.Results-Current vulvar pain of at least 6 months duration was reported by 3.8% of respondents, with a 9.9% lifetime prevalence. Forty-five percent of women with pain reported an adverse effect on their sexual life and 27% an adverse effect on their life style. Cases more frequently reported repeated urinary tract infections (OR, 6.15; 95% CI,) and yeast infections (OR, 4.24; 95% CI,). Associations existed with chronic fatigue syndrome (OR, 2.78; 95% CI, 1.33-6.19), fibromyalgia (OR, 2.15; 95% CI, 1.06-4.36), depression (OR, 2.99; 95% CI,, and irritable bowel syndrome (OR, 1.86; 95% CI,).Conclusions-Lifetime chronic vulvar pain was less prevalent in this national sample of women than previous data suggest and was correlated with several co-morbid chronic medical conditions and substantial reduction in self-reported quality of life.