PSX-B-10 Characterization and Comparison of the Bovine Nasal Microbiome to Exposure of Persistently Infected Bovine Viral Diarrhea Virus

Abstract

Bovine viral diarrhea virus (BVDV) is a critical disease negatively impacting many facets of the cattle production system. Cattle infected with the persistent (PI) form of BVDV are the primary reservoirs and source of transmission and may be implicated with an increased risk of bovine respiratory disease (BRD) to exposed calves in feedlots. To date, little to no data exists regarding characterization of the effects PI-BVDV has on the bovine nasal microbiome, an important feature for bovine health. To investigate these effects, Angus-crossbred beef calves were selected and randomly assigned to either continuous exposure (E, n = 3) or non-exposure (NE, n = 3) to an auction market sourced PI-BVDV positive (PI, n = 1) calf from weaning to 112 days post-weaning. Nasal swab samples were collected upon weaning, 56 days post-weaning, and 112 days post-weaning. Next generation sequencing was used to measure the effects of PI-BVDV on the nasal microbiome. Alpha diversity, including Shannon Index and Observed OTUs, and beta diversity, including Bray-Curtis and Jaccard distances, were not different (P > 0.05) between treatments. Next, community structure was assessed at the amplicon sequence variant (ASV) level and LEfSe was used to identify the bacterial biomarkers for each treatment. Results indicated that ASV5-Mycoplasma could be a biomarker for the PI and E treatments due to its high relative abundance in each when compared with the NE treatment. Overall, ASV5-Mycoplasma was identified as a potential biomarker for the prediction and diagnosis of persistently infected bovine viral diarrhea virus among both calves infected with and exposed to the virus and may be an additional biomarker for the increased risk of bovine respiratory disease in exposed feedlot cattle.