PSVII-6 Effects of 25-hydroxycholecalciferol supplementation on gene expression of feedlot cattle

Abstract

The objective was to evaluate the effects of 25-hydroxycholecalciferol (HyD®, DSM Produtos Nutricionais Brasil S.A.) supplementation in expression of genes related to anabolism and catabolism of feedlot cattle. A total of 120 Nellore bulls (IBW = 370±20 kg) were distributed in 24 fully roofed-pens with concrete floor (5 animals/pen) in a randomized complete block design (8 pens/treatment). The treatments were T1 (control): no supplementation of HyD®; T2: HyD® supplementation at 1 mg/animal/day; T3: HyD® supplementation at 3 mg/animal/day. Basal diet was formulated to meet requirements of finishing bulls, considering an ADG of 1.6 kg/day (Level 2 Nutrition System, Fox et al., 2004). Adaptation to diets followed a step-up scheme for 14 days, with concentrate inclusion being gradually increased from 76 to 91% DM. Finishing diets were offered from 15th to 100th day of feedlot period and animals fed twice a day (0800 and 1500), with bunks managed for a maximum of 5% orts. After slaughter, muscle samples were collected for quantitative evaluation of gene expression using RT-qPCR method, considering the following genes: SOD1 (antioxidant marker), IGF1, IGF2 and MTOR (anabolism), FOXO1, MURF1, Atrogin-1 and MSTN (catabolism). Statistical analysis was performed using PROC MIXED of SAS® and means compared by Tukey test at 5% probability. No significant differences among treatments were observed for SOD1, FOXO1, MURF1 and Atrogin-1 expression (P >0.05). However, tendencies (0.05 > P ≤0.10) could be observed for IGF1, IGF2, MTOR and MSTN expression in animals receiving HyD®, regardless of dosage. These results indicate a positive effect of HyD® on muscular anabolism and protein synthesis on feedlot finishing cattle. Moreover, the greater expression on MSTN suggest a higher protein turnover and muscular growth regulation. In conclusion, HyD® supplementation increased expression of genes correlated to muscular growth and protein synthesis, being a viable technology for beef cattle finished in feedlot systems.

Influences of a Supplemental Blend of Essential Oils Plus 25-Hydroxy-Vit-D3 and Zilpaterol Hydrochloride (β2 Agonist) on Growth Performance and Carcass Measures of Feedlot Lambs Finished under Conditions of High Ambient Temperature

“…Supra-supplementation with vitamin D3 resulted in increased carcass weight and dressing percentage [11,12]. This may be attributable to increased expression of genes affecting muscle growth and protein synthesis [58,59]. However, in the present study there were no appreciable effects of D3 in combination with EO on carcass measures.…”

Influence of a Supplemental Blend of Essential Oils Plus 25-hydroxy-vitamin-D3 on Feedlot Cattle Performance during the Early-growing Phase under Conditions of High-ambient Temperature

“…Compared with MON, EO+HyD increased (8.3%, P<0.01) ADG. This enhancement in ADG may reflect, in part, a potentiating effect of HyD on net protein retention and hence, lean tissue growth (Carvalho and Perdigao, 2019;Martins et al, 2020). Toseti et al (2020) observed that in Nellore bulls supplemented with EO (CRINA Ruminants®) plus exogenous á-amylase, ADG was 8.5% greater than observed in bulls fed MON.…”

Comparing Blend of Essential Oils Plus 25-Hydroxy-Vit-D3 Versus Monensin Plus Virginiamycin Combination in Finishing Feedlot Cattle: Growth Performance, Dietary Energetics, and Carcass Traits

“…Additionally, they are observed to enhance immune response and reduce cellular oxidative stress [11,47,48]. Vitamin D3 at high levels of supplementation (i.e., ≥1 mg/animal/day) is active in immune and endocrine functions [49] and in mechanisms that promote protein accretion [17]. All of these enhancements associated with supplemental MON, VM, EO, and HyD help to facilitate feedlot cattle energetic efficiency.…”

“…In contrast, daily supplementation at higher levels (~1 mg/animal) increased carcass dressing percentage (+0.54 percentage points) [16] and carcass weight (+1.49%) [15] in Nellore cattle during a finishing period of at least 90 days. These effects can be partially explained by the potentiating effects of higher-level vitamin D3 supplementation on protein accretion [17]. The basis for the increase in LM area in cattle fed EO + HyD is not clear.…”

“…In recent reports, 25-hydroxyvitamin-D3 (HyD) supplemented at 0.10 mg/kg of diet tended to increase average daily gain (ADG) [14], carcass weight [15], and dressing percentage [16]. Researchers attributed these results to the effects of HyD on protein accretion [17]. Due to the chemical nature and the mechanism of action of EO and HyD, their combination could be complementary.…”