pSRD191, a new member of RepL replicating plasmid family from Selenomonas ruminantium

Šprincová, Adriana; Javorský, P.; Pristaš, Peter

doi:10.1016/j.plasmid.2004.11.004

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…3 , Table 1 ). Replication proteins of the RepL family are frequently found in small cryptic or erythromycin resistance encoding RCR plasmids previously identified in Staphylococcus and Bacillus species [33] . The presence of more than one replication protein has previously been reported for other plasmids, such as the Streptococcus faecalis plasmid pAMα1 [34] and the Bacillus plasmid pTB19 [35] , [36] .…”

Section: Resultsmentioning

confidence: 99%

A Novel Erythromycin Resistance Plasmid from Bacillus Sp. Strain HS24, Isolated from the Marine Sponge Haliclona Simulans

et al. 2014

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A better understanding of the origin and natural reservoirs of resistance determinants is fundamental to efficiently tackle antibiotic resistance. This paper reports the identification of a novel 5.8 kb erythromycin resistance plasmid, from Bacillus sp. HS24 isolated from the marine sponge Haliclona simulans. pBHS24B has a mosaic structure and carries the erythromycin resistance gene erm(T). This is the first report of an erythromycin resistance plasmid from a sponge associated bacteria and of the Erm(T) determinant in the genus Bacillus.

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Section: Resultsmentioning

confidence: 99%

A Novel Erythromycin Resistance Plasmid from Bacillus Sp. Strain HS24, Isolated from the Marine Sponge Haliclona Simulans

et al. 2014

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“…Plasmid replication proteins are listed with the plasmids that encode them. The RepL consensus sequence is from Sprincova et al (2005) . The scale bar indicates the distance for 0.5 amino acid substitutions per site.…”

Section: Resultsmentioning

confidence: 99%

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature

Groom

Chung

Olson

et al. 2016

Metabolic Engineering Communications

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Clostridium thermocellum is a leading candidate for the consolidated bioprocessing of lignocellulosic biomass for the production of fuels and chemicals. A limitation to the engineering of this strain is the availability of stable replicating plasmid vectors for homologous and heterologous expression of genes that provide improved and/or novel pathways for fuel production. Current vectors relay on replicons from mesophilic bacteria and are not stable at the optimum growth temperature of C. thermocellum. To develop more thermostable genetic tools for C. thermocellum, we constructed vectors based on the hyperthermophilic Caldicellulosiruptor bescii replicon pBAS2. Autonomously replicating shuttle vectors based on pBAS2 reproducibly transformed C. thermocellum at 60 °C and were maintained in multiple copy. Promoters, selectable markers and plasmid replication proteins from C. bescii were functional in C. thermocellum. Phylogenetic analyses of the proteins contained on pBAS2 revealed that the replication initiation protein RepL is unique among thermophiles. These results suggest that pBAS2 may be a broadly useful replicon for other thermophilic Firmicutes.

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“…An abundant plasmid population was detected in S. ruminantium strain 19D, consisting of six plasmids ranging in size from 1.4 to more than 20 kb. The two smallest plasmids, pSRD191 (GenBank AY572460) and pSRD192 (DQ186900), were completely sequenced and characterized in our laboratory (Sprincova et al, 2005;Ivan et al, 2006). Presence of single-stranded plasmid intermediates, the hallmark of RC replication, was demonstrated by DNA-DNA hybridization (Pristas et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Variability of putativerepgene cassettes inSelenomonas ruminantiumplasmids

Fecskeová

Ivan

Javorský

et al. 2012

FEMS Microbiol Lett

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Characteristic feature of the most of Selenomonas ruminantium cryptic plasmids is the presence of short, conserved sequences encompassing the gene for replication protein creating a potential rep gene cassette. PCR-based experiment was designed to analyse the genetic organization of putative plasmid rep modules and to assess S. ruminantium plasmid biodiversity. Analysed PCR amplicons contained single open reading frames encoding for putative replication proteins. While most of the derived protein sequences were often found to be conserved among putative plasmid molecules, at noncoding regions, genetic variability was observed to various extents. Complete nucleotide sequence of a plasmid was determined that contained probably a new rep gene only distantly related to known selenomonas Rep proteins but at noncoding regions shared high homology with already known plasmids. Our results document considerable structural instability and sequence variability of analysed rep gene cassettes and suggest a modular structure of S. ruminantium plasmids potentially accessible for rep gene module exchanges.

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pSRD191, a new member of RepL replicating plasmid family from Selenomonas ruminantium

Cited by 9 publications

References 46 publications

A Novel Erythromycin Resistance Plasmid from Bacillus Sp. Strain HS24, Isolated from the Marine Sponge Haliclona Simulans

A Novel Erythromycin Resistance Plasmid from Bacillus Sp. Strain HS24, Isolated from the Marine Sponge Haliclona Simulans

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature

Variability of putativerepgene cassettes inSelenomonas ruminantiumplasmids

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