pSlugS158 immunohistochemistry is a novel promising mitotic marker for FFPE samples: a pilot study

Woo, Chang Gok; Son, Seung-Myoung; Lim, Young Hyun; Lee, Dakeun; Park, Jung-Jin; Kim, Eung‐Gook; Shin, Eun‐Young; Lee, Ok-Jun

doi:10.1007/s00428-021-03201-7

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“…[11][12][13][14][15] In a previous study, we observed that the expression of pSlug S158 is regulated during the cell cycle, and pSlug S158 gets accumulated during mitosis. 7 Expression of pSlug S158 in IHC suggested that it is a useful mitotic marker.…”

Section: Discussionmentioning

confidence: 99%

“…5,6 In a previous study, we showed that PAK4 mediated site-specific phosphorylation of Slug at Ser-158 (pSlug S158 ) is upregulated during the M phase. 7 In addition, immunohistochemistry (IHC) for pSlug S158 detected mitotic figures in normal tissues and neoplasms. Herein, we aimed to evaluate if pSlug S158 for staining for mitotic counts could be used to grade meningioma.…”

Section: Introductionmentioning

confidence: 99%

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pSlug^S158 Immunohistochemistry is Useful in Grading Meningioma

et al. 2022

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Snail family transcriptional repressor 2 (SNAI2, Slug) is a transcription factor that belong to the Slug/Snail superfamily. Site specific phosphorylation of slug (pSlugS158) is detected during the M phase, and thus, this phosphorylated protein is considered a novel marker for detecting mitotic figures. Herein, we investigated whether the detection of mitosis using pSlugS158 expression can be used in the histological grading of meningioma. We performed immunohistochemistry for pSlugS158 and PHH3 in tissue samples of 61 patients with meningioma and examined the association between mitotic counts using pSlugS158 and recurrence-free survival (RFS). The nuclear expression of pSlugS158 was observed in the cell with mitotic figures. Tumor grading based on pSlugS158 was significantly associated with the RFS ( p < .001). It can be concluded that pSlugS158 is a useful and practical marker to detect mitosis and seems to be reliable for the counting of mitoses in histological grading of meningioma.

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